Detection and Sequence Analysis of Lily mottle virus in Narcissus pseudonarcissus from the Netherlands by RT-PCR Technique

ACTA HORTICULTURAE SINICA ›› 2008, Vol. 35 ›› Issue (12): 1843-1848.

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Detection and Sequence Analysis of Lily mottle virus in Narcissus pseudonarcissus from the Netherlands by RT-PCR Technique

LIU Bo¹,MING Jun^1*,LIU Chun¹,LUO Feng-xia²,WANG Chun-cheng³,SHAN Hong-chen³,WANG Xiao-wu¹,and MU Ding¹

(¹Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China; ²Colloge of Technology and Horticulture of Jinling, Nanjing 210038；³Beijing Municipal Bureau of Parks and Afforestation Division of Flower Industry, Beijing 100029，China)

Received:2008-07-08 Revised:2008-09-08 Online:2008-12-25 Published:2008-12-25
Contact: MING Jun

Abstract

Abstract:

Lily mottle virus（LMoV）was detected in Narcissus pseudonarcissus using one set of specific primer by reverse transcription-polymerase chain reaction (RT-PCR). A 553bp DNA fragment was amplified from the sample of ‘Pink-charm’ using primers L1 (5'-TGGGCACCTTGTGAATTAC-3') and L2 (5'- TGCTGTATGCCTCTCCGTGC-3'). The primer was designed according to the published sequence of the coat protein of LMoV in the GenBank. Nucleotide sequence of the fragment（GenBank Accession No. EU167936）showed more than 98% identity with other isolates（GenBank Accession Nos.:AJ748256；AJ564636；AB053256；AF531458；AJ564637；AJ748257）. Remarkably high Similarity in the nucleotide has been observed despite of their different origins. Besides, amino acid sequence of the amplified virus fragments（GenBank Accession No. ABW16938）is overlapped by the amino acid sequence of LMoV Coat protein（GenBank Accession No .NP-945145）.

Key words: Narcissus pseudonarcissus, Lily mottle virus, RT-PCR, Virus detection

CLC Number:

S 682

LIU Bo;MING Jun;LIU Chun;LUO Feng-xia;WANG Chun-cheng;SHAN Hong-chen;WANG Xiao-wu;and MU Ding . Detection and Sequence Analysis of Lily mottle virus in Narcissus pseudonarcissus from the Netherlands by RT-PCR Technique[J]. ACTA HORTICULTURAE SINICA, 2008, 35(12): 1843-1848.

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Detection and Sequence Analysis of Lily mottle virus in Narcissus pseudonarcissus from the Netherlands by RT-PCR Technique

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