Acta Horticulturae Sinica ›› 2021, Vol. 48 ›› Issue (12): 2481-2496.doi: 10.16420/j.issn.0513-353x.2020-0717

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Genome-wide Identification and Expression Analysis of TCP Family in Dimocarpus longan

JIANG Mengqi1, XUE Xiaodong1, SU Liyao1, CHEN Yan1, ZHANG Shuting1, LI Xiaofei1, WANG Peiyu2, ZHANG Zihao1, LAI Zhongxiong1, LIN Yuling1,*()   

  1. 1Institute of Horticultural Biotechnology,Fujian Agriculture and Forestry University,Fuzhou 350002,China
    2Sanming Academy of Agricultural Sciences,Sanming,Fujian 365000,China
  • Received:2021-03-11 Revised:2021-07-30 Published:2022-01-04
  • Contact: LIN Yuling


To study the potential function of longan TCP gene family,bioinformatics methods were used to perform the genome-wide identification of family in Dimocarpus longan TCP genes(DlTCP). The expression patterns of DlTCP in longan somatic embryo development and their responses to ABA and MeJA were detected by the real-time quantitative PCR(qRT-PCR). In this study,20 longan TCP gene family members were identified in the longan genome database. These DlTCP proteins contained 169- 540 amino acid residues,with molecular weights ranging from 39.6 kD to 57.2 kD,and theoretical isoelectric points ranging from 5.90-9.45. The prediction of subcellular localization showed that 18 members were located in the nucleus. DlTCP were distributed on 11 of the 15 chromosomes of longan. Synteny analysis revealed that 12 of DlTCP were involved in fragment replication events. Promoter cis-acting elements analysis showed that DlTCP family members contained hormone response cis-acting elements and regulate growth and development cis-acting elements. The longan TCP family members can be divided into two categories in phylogenetic evolution,and they all had typical TCP domains. QRT-PCR results showed that DlTCP2/4a/4b/13/17/18/19/23 were highly expressed in the embryogenic callus(EC)stage,DlTCP5b/8 were highly expressed in the incomplete compact pro-embrogenic cultures(IcpEC)stage,and only DlTCP20a was highly expressed in the globular embryos(GE)stage,indicating that the higly expression of DlTCP may contribute to the morphogenesis of early somatic embryos in longan. In addition,the family members also exhibited different expression patterns after exogenous hormones treatments. After exogenous ABA induction,the expression of four DlTCP members was significantly down regulated,and four were up-regulated;After MeJA induction,the expression of three members was significantly down-regulated and four up-regulated,indicating that DlTCP members can respond to ABA and MeJA.

Key words: Dimocarpus longan, TCP transcription factor, gene famliy identification, ABA, MeJA, qRT-PCR

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