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ACTA HORTICULTURAE SINICA ›› 2008, Vol. 35 ›› Issue (10): 1447-1454.

• 蔬菜 • Previous Articles     Next Articles

Simple Sequence Repeat(SSR)as Anchor Markers in Constructing a Molecular Genetic Map of Chinese Cabbage (Brassica rapa L. ssp pekinensis)

YU Ren-bo1,2;YU Shuan-cang2;QI Jia-ni2,3;ZHANG Feng-lan2*;YU Yang-jun2;ZHAO Xiu-yun2;and ZHANG De-shuang2
  

  1. (1College of Life ScienceCapital Normal University, Beijng 100037, China; 2Vegetable Reaserch Center, Beijing Academy Agricultural and Frestry Science, Beijing 100097, China; 3Department of Horticulture, Shenyang Agriculture University, Shenyang, 100161, China)
  • Received:2008-06-18 Revised:2008-09-10 Online:2008-10-25 Published:2008-10-25
  • Contact: ZHANG Feng-lan

Abstract: In this paper, a DH population including 100 lines was used to construct a detailed genetic map to establish the identity of linkage groups corresponding to reference map of Brassica A genome. A set of SSR and STS markers provided anchors to previously published linkage maps for B. rapa and B. napus, and was used to designate linkage groups. Out of 230 SSR markers and 8 STS markers, 67 markers showed polymorphism between 2 parents and were analyzed in DH population. In addition, 448 previously mapped molecular markers,including 263 AFLP markers, 150 RAPD markers, 17 SSR markers, 3 SCAR markers, 14 isozyme markers and a morphological marker were integrated to a genetic map. This map contains 497 loci, and covered 1 086.7 cM with an average distance 2.19 cM. In this map, 36 markers previously published in reference map, including 34 SSR markers and 2 STS markers, were distributed in 10 linkage groups. Accordingly, these linkage groups were named following the A1~A10 of reference map, and were assigned to corresponding chromosomes.

Key words: Chinese cabbage, genetic map, SSR, anchor markers

CLC Number: