The Effect of Camalexin on Chinese Cabbage Resistance to Clubroot

doi:10.16420/j.issn.0513-353x.2021-0591

Acta Horticulturae Sinica ›› 2022, Vol. 49 ›› Issue (8): 1689-1698.doi: 10.16420/j.issn.0513-353x.2021-0591

• Research Papers • Previous Articles Next Articles

The Effect of Camalexin on Chinese Cabbage Resistance to Clubroot

WANG Yu¹, ZHANG Xue¹, ZHANG Xueying¹, ZHANG Siyu¹, WEN Tingting¹, WANG Yingjun¹, GAN Caixia², PANG Wenxing¹^,^**()

1. Department of Horticulture,Shenyang Agricultural University,Shenyang 110866,China
2. Institute of Cash Crops,Hubei Academy of Agricultural Sciences,Wuhan 430064,China

Received:2022-02-25 Revised:2022-06-14 Online:2022-08-25 Published:2022-09-05
Contact: PANG Wenxing E-mail:pwxsyau@syau.edu.cn

Abstract

Abstract:

To investigate the resistant effects of camalexin to Plasmodiophora brassicae in Chinese cabbage,treatments with five amount of camalexin were carried out by P. brassicae inoculation on a Chinese cabbage susceptible inbred line‘BJN3-1’. Results indicated that the control showed the visually susceptible symptoms. But the treatments with different amount of camalexin after 21 days treatment can significantly inhibit the invasion of P. brassicae to reduce the disease index of clubroot. The inhibition of P. brassicae infection was enhanced with the increase of camalexin application. Furthermore,the genes involved in synthesis pathways of jasmonic acid,salicylic acid,ethylene and pathogenesis-related proteins were significantly up-regulated after 14-21 days under camalexin treatment. These indicated that the application of camalexin can induce the host responses to P. brassicae invasion,and improve resistance. However,the effects of all camalexin treatments on clubroot development were reduced after 28 days treatment which may be related to the microbial detoxification activities of P. brassicae.

Key words: Chinese cabbage, clubroot disease, Plasmodiophora brassicae, camalexin

CLC Number:

S634.1

WANG Yu, ZHANG Xue, ZHANG Xueying, ZHANG Siyu, WEN Tingting, WANG Yingjun, GAN Caixia, PANG Wenxing. The Effect of Camalexin on Chinese Cabbage Resistance to Clubroot[J]. Acta Horticulturae Sinica, 2022, 49(8): 1689-1698.

Figures/Tables 7

References 31

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用途 Usage	基因 Gene	引物序列（5′-3′） Primer sequence
内参基因 Internal genes	GAPDH	F：CAGGTTTGGAATTGTCGAGG	R：GAGCTGTGGAAGCACCTTTC
茉莉酸途径	PDF1.2	F：ACCATGTCCCACTTGGCTTCT	R：ATCACCCTTATCTTCGCTGCTC
Jasmonic acid synthesis	AOS	F：CCTAGTCGCAAGCGGTTGAT	R：AGGGATTGAGCAGATGGAGC
pathway	COI1	F：CCTCTTTGTGAGACTAAGCCTTCT	R：AACTATGTTGTCTGGGCCTTTC
	JAZ	F：GGCCACCTTCATAATGTCCTC	R：TGCTAACCCTTCCGATACCTC
水杨酸途径	NPR1	F：TTGGACCTCACTGATCCTGTTTT	R：ACGGTATTTCCCACGCTGCTC
Salicylic acid synthesis pathway	ESD5	F：CTTGGGAAGGTTACGGTTCG	R：CATCATCTACTGTGCTACCTCCAT
	WRKY70	F：ACCACAACCATAAATAGCCTCA	R：AACACTAAAGAAGTATCACCCAAGA
	TGA	F：TTGCGATGATTGCTGGAGGT	R：TATCGGGCATGTGGCGAACT
乙烯途径	CTR1	F：ACAGGAGATGTATTGGGAGGGA	R：GGACAGTGAAGGTTTGCGATTT
Ethylene synthesis pathway	ETR	F：CAACAGGATGCTGCTGACGAA	R：GCTGGACAGGGAGATGGGATT
	EIN2	F：CCACTGCTAGTGTTGTCCCTTA	R：TGTCTGCGATTCTTGATGAGTT
病程蛋白相关基因 Pathogenesis related proteins	PR1	F：CCCTCGAGAGCTCAAAACAG	R：TCTCGTTGACCCAAAGGTTC
	PR2	F：CTCGGAGTTCCACCCTTCACA	R：CAAACCTCTTCGACGCCAATC
	PR5	F：AATGAACCAGGATGGTCAGG	R：CGGTGACGGTGCATTTTAG
	PR9	F：AGCTGCTGGATGTAACCTGAAT	R：CCATGATATGGGCAATGACTGA

用途 Usage	基因 Gene	引物序列（5′-3′） Primer sequence
内参基因 Internal genes	GAPDH	F：CAGGTTTGGAATTGTCGAGG	R：GAGCTGTGGAAGCACCTTTC
茉莉酸途径	PDF1.2	F：ACCATGTCCCACTTGGCTTCT	R：ATCACCCTTATCTTCGCTGCTC
Jasmonic acid synthesis	AOS	F：CCTAGTCGCAAGCGGTTGAT	R：AGGGATTGAGCAGATGGAGC
pathway	COI1	F：CCTCTTTGTGAGACTAAGCCTTCT	R：AACTATGTTGTCTGGGCCTTTC
	JAZ	F：GGCCACCTTCATAATGTCCTC	R：TGCTAACCCTTCCGATACCTC
水杨酸途径	NPR1	F：TTGGACCTCACTGATCCTGTTTT	R：ACGGTATTTCCCACGCTGCTC
Salicylic acid synthesis pathway	ESD5	F：CTTGGGAAGGTTACGGTTCG	R：CATCATCTACTGTGCTACCTCCAT
	WRKY70	F：ACCACAACCATAAATAGCCTCA	R：AACACTAAAGAAGTATCACCCAAGA
	TGA	F：TTGCGATGATTGCTGGAGGT	R：TATCGGGCATGTGGCGAACT
乙烯途径	CTR1	F：ACAGGAGATGTATTGGGAGGGA	R：GGACAGTGAAGGTTTGCGATTT
Ethylene synthesis pathway	ETR	F：CAACAGGATGCTGCTGACGAA	R：GCTGGACAGGGAGATGGGATT
	EIN2	F：CCACTGCTAGTGTTGTCCCTTA	R：TGTCTGCGATTCTTGATGAGTT
病程蛋白相关基因 Pathogenesis related proteins	PR1	F：CCCTCGAGAGCTCAAAACAG	R：TCTCGTTGACCCAAAGGTTC
	PR2	F：CTCGGAGTTCCACCCTTCACA	R：CAAACCTCTTCGACGCCAATC
	PR5	F：AATGAACCAGGATGGTCAGG	R：CGGTGACGGTGCATTTTAG
	PR9	F：AGCTGCTGGATGTAACCTGAAT	R：CCATGATATGGGCAATGACTGA

Camalexin/（ng · mL^-1）	病情指数 Disease index
Camalexin/（ng · mL^-1）	7 d	14 d	21 d	28 d
0	0	0	74.33 ± 4.00 a	97.00 ± 1.00 a
250	0	0	74.67 ± 2.50 a	95.67 ± 1.35 a
500	0	0	65.68 ± 2.90 b	95.33 ± 1.68 a
1 000	0	0	63.22 ± 2.55 b	96.58 ± 2.58 a
2 000	0	0	44.50 ± 1.76 bc	93.05 ± 2.31 a
5 000	0	0	38.12 ± 2.21 bc	81.25 ± 3.50 b

Camalexin/（ng · mL^-1）	病情指数 Disease index
Camalexin/（ng · mL^-1）	7 d	14 d	21 d	28 d
0	0	0	74.33 ± 4.00 a	97.00 ± 1.00 a
250	0	0	74.67 ± 2.50 a	95.67 ± 1.35 a
500	0	0	65.68 ± 2.90 b	95.33 ± 1.68 a
1 000	0	0	63.22 ± 2.55 b	96.58 ± 2.58 a
2 000	0	0	44.50 ± 1.76 bc	93.05 ± 2.31 a
5 000	0	0	38.12 ± 2.21 bc	81.25 ± 3.50 b

The Effect of Camalexin on Chinese Cabbage Resistance to Clubroot

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