ISSR Analysis of Cultiva ted Auricularia auricula

ACTA HORTICULTURAE SINICA ›› 2007, Vol. 34 ›› Issue (4): 935-940.

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ISSR Analysis of Cultiva ted Auricularia auricula

LI Hui-ping;HUANG Chen-yang;CHEN Qiang;ZHANG Jin-xia^*

( Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081China)

Received:1900-01-01 Revised:1900-01-01 Online:2007-08-25 Published:2007-08-25

Abstract

Abstract: Genetic diversity was assessed in 21 cultivated strains of A. auricula by ISSR ( inter simple
sequence repeats). Ten primerswere screened out from twenty ISSR primers in this study. One hundred and
eighty five loci were produced from the primers, of which 181 loci (97.84% ) were polymorphic in the organism. The size of these loci ranged from 200 bp to 3 000 bp. High genetic diversity of A. auricula in China was
detected: the observed number of alleles (Na) averaged 1.9784 and the effective number of alleles (Ne) was
1.2396, for every locus; Neips genetic diversity ( h) was estimated to be 0.2732, while Shannonps information index ( I) was 0.4278, on average1 The Neips ( 1972) genetic distance among strains ranged from 0.36 to
0.55, but the gene flow (Nm ) was 2.7528. Analysis showed that there were high genetic diversity and large
spawns exchange among p rovinces for A. auricula in China.

Key words: ISSR, Jelly fungus, Genetic diversity, Genetic marker

LI Hui-ping;HUANG Chen-yang;CHEN Qiang;ZHANG Jin-xia. ISSR Analysis of Cultiva ted Auricularia auricula[J]. ACTA HORTICULTURAE SINICA, 2007, 34(4): 935-940.

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ISSR Analysis of Cultiva ted Auricularia auricula

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