Evaluation of Application of SRAP on Analysis of Genetic Diversity in Cultivars of Allium fistulosum L.

ACTA HORTICULTURAE SINICA ›› 2007, Vol. 34 ›› Issue (4): 929-934.

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Evaluation of Application of SRAP on Analysis of Genetic Diversity in Cultivars of Allium fistulosum L.

LI Hui-zhi; YIN Yan-p ing^*; ZHANG Chun-qing;ZHANG Min;LI Jian-min

(Agronomy College, Shandong Agricultural University, State Key Laboratory of Crop Biology, Tai'an, Shandong 271018,China)

Received:1900-01-01 Revised:1900-01-01 Online:2007-08-25 Published:2007-08-25

Abstract

Abstract: In order to evaluate the app lication of SRAP in identification and genetic relationship analysis
on cultivars ofA llium f istulosum L. , 20 cultivarswere used in this experiment. We investigated the morphological characters and analyzed the genetic diversity of 20 cultivars with SRAP markers. 256 p rimer pairs were
tested, 161 (62.9% ) of which generated polymorphic bands. A total of 336 polymorphic bands were observed. The number of polymorphic bands detected by an individual p rimer pair ranged from one to six, with
an average number of 2.1. Genetic similarity (GS) coefficients among 20 cultivars based on the SRAP data
ranged from 0.464 to 0.938, with a mean GS coefficient of 0.703. A dendrogram was generated using cluster
analysis, which revealed phylogenetic relationship s among 20 cultivars. The result was in agreement with the
common classification based on morphologic traits, which suggested that SRAP marker could be reliably used
in cultivar identification and genetic diversity analysis of A. fistulosum L.

Key words: Allium fistulosum L., Cultivar, SRAP, Genetic diversity, Application

LI Hui-zhi;YIN Yan-p ing;ZHANG Chun-qing;ZHANG Min;LI Jian-min. Evaluation of Application of SRAP on Analysis of Genetic Diversity in Cultivars of Allium fistulosum L.[J]. ACTA HORTICULTURAE SINICA, 2007, 34(4): 929-934.

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Evaluation of Application of SRAP on Analysis of Genetic Diversity in Cultivars of Allium fistulosum L.

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