Isolation of Fe Deficiency-induced Genes by Suppression Subtractive Hybridiza tion in Malus xiaojinensis

ACTA HORTICULTURAE SINICA ›› 2007, Vol. 34 ›› Issue (3): 555-560.

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Isolation of FeDeficiency-induced Genes by Suppression Subtractive Hybridiza tion in Malus xiaojinensis

ZHANG Yu-gang^1,2;XU Xue-feng¹;LI Tian-zhong¹;WANG Yi¹;KONG Jin¹;HAN Zhen-hai^1*

(¹ Institute for Horticultural Plants, China Agricultural University, Beijing 100094, China; ²Horticultural College, QingdaoAgricultural University, Qingdao, Shandong 266109,China)

Received:1900-01-01 Revised:1900-01-01 Online:2007-06-25 Published:2007-06-25

Abstract

Abstract: In order to isolate genes involved in Fe nutrition, supp ression subtractive hybridization ( SSH)
was performed using mixed cDNAs p repared from Malus xiaojinensis roots treated with Fe deficiency as tester
and treated with Fe sufficiency as drivers. A forward subtractive cDNA library was constructed, from which
1 152 recombinant colonieswere p icked and amp lified. Through differential screening of the subtractive cDNA
library, 105 exp ress sequence tags (ESTs) were identified as Fe deficiency stress induced. Of the 105 ESTs,
50 contain regionswith 60% - 90% identity to their homolog in GenBank, 32 are expected to be novel genes,
23 are repeated fragments to other genes. The ESTswith significant p rotein homologywere sorted into 9 func2
tional categories.

Key words: Apple, Malus xiaojinensis, Suppression subtractive hybridization ( SSH), cDNA library, Differential screen

ZHANG Yu-gang;XU Xue-feng;LI Tian-zhong;WANG Yi;KONG Jin;HAN Zhen-hai. Isolation of FeDeficiency-induced Genes by Suppression Subtractive Hybridiza tion in Malus xiaojinensis[J]. ACTA HORTICULTURAE SINICA, 2007, 34(3): 555-560.

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Isolation of FeDeficiency-induced Genes by Suppression Subtractive Hybridiza tion in Malus xiaojinensis

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