Analysis of Ploidy and Homozygous Genotype of Apple Plants Obtained byAnther Culture

ACTA HORTICULTURAE SINICA ›› 2007, Vol. 34 ›› Issue (2): 481-484.

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Analysis of Ploidy and Homozygous Genotype of Apple Plants Obtained byAnther Culture

ZHANG Li-yi¹, YANG Zhen-ying¹,CONG Pei-hua¹,ZHANG Kai-chun² , LIU Jian-li³,and XUE Guang-rong¹

(¹Institute of Pom ology, Chinese Academ y of Agricultural Sciences, Xingcheng, L iaoning 125100, China; ²Institute of Forestry and Pomology, Beijing Academy of Agriculture and Forestry Sciences, B eijing 100093, China; ³Departm ent of Life Sciences, the Second Northwest University forM inorities, Yinchuan 750021, China)

Received:2006-07-21 Revised:2006-12-29 Online:2007-04-25 Published:2007-04-25

Abstract

Abstract: In this paper, the ploidy and homozygous genotype of three apple plants obtained by anther culture were analyzed by using methods of the flow cytometric analysis and AS-PCR marker, in order to confirm their hap loid origin. The results have proved that they all have a true homozygous status by AS-PCR analysis although they are now dip loid, trip loid and tetrap loid respectively by Ploidy Analyser, which is possibly regarded as a result of spontaneous polyp loidisation from hap loid cell during tissue culture.

Key words: Apple, Anther culture, AS-PCR, Ploidy, Homozygous genotype

CLC Number:

S 661.1

ZHANG Li-yi;YANG Zhen-ying;CONG Pei-hua;ZHANG Kai-chun;LIU Jian-li;and XUE Guang-rong. Analysis of Ploidy and Homozygous Genotype of Apple Plants Obtained byAnther Culture[J]. ACTA HORTICULTURAE SINICA, 2007, 34(2): 481-484.

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Analysis of Ploidy and Homozygous Genotype of Apple Plants Obtained byAnther Culture

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