Cloning and Expression Analysis of the SLF and S-RNase Genes in Almond

ACTA HORTICULTURAE SINICA ›› 2006, Vol. 33 ›› Issue (6): 1185-1192.

• 研究论文 • Previous Articles Next Articles

Cloning and Expression Analysis of the SLF and S-RNase Genes in Almond

Guo Zhenyu^{1, 2};Chang Fengqi¹;Xie Hua¹;Xu Yong¹;Ma Rongcai^{1, 2*}

(¹Beijing Agro-Biotechnology Research Center, Beijing 100089, China; ²Life Science College, Capital Normal University, Beijing 100037, China)

Received:2005-11-11 Revised:2006-07-26 Online:2006-12-25 Published:2006-12-25

Abstract

Abstract: Using RT-PCR and Rapid Amplification of cDNA Ends (RACE) techniques, cDNAs for one novel SLF (S Locus F-box) ( PdSLF1) gene and two novel alleles of S-RNase genes ( PdSm and PdSn) were cloned from almond ( Prunus dulcis Mill. ) cultivar‘Pioneer’, and their expression patterns were investigated in anthers, pistils, petals, sepals and leaves. PdSLF1 was 1 331 bp in length encoding a protein of 376 amino acids. The comp lete cDNA of PdSm was 826 bp encoding 228 amino acids and PdSn 878 bp encoding 227 amino acids. Compared the deduced p rotein sequenceswith those registered in GenBank, PdSLF1, PdSm and PdSn proteins shared high identities with other SLF (70.2% - 84.8% ) and S-RNase (59% - 83.9% ) proteins, respectively. Furthermore, the results of semi-quantitative RT2PCR analysis showed that PdSLF1 expressed exclusively in anthers, and two alleles of S-RNase, PdSm and PdSn, exclusively in pistils, which suggested that theymay participate in self-incompatibility in almond.

Key words: Almond, Self-incompatibility, Gene, Cloning, S-RNase

CLC Number:

S662.1

Guo Zhenyu;;Chang Fengqi;Xie Hua;Xu Yong;Ma Rongcai;. Cloning and Expression Analysis of the SLF and S-RNase Genes in Almond[J]. ACTA HORTICULTURAE SINICA, 2006, 33(6): 1185-1192.

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Cloning and Expression Analysis of the SLF and S-RNase Genes in Almond

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