Detection and Sequence Analysis of Hop stunt viroid in Apricot and Plum

ACTA HORTICULTURAE SINICA ›› 2006, Vol. 33 ›› Issue (6): 1193-1198.

• 研究论文 • Previous Articles Next Articles

Detection and Sequence Analysis of Hop stunt viroid in Apricot and Plum

Yang Yuanai^{1, 2};Li Shifang^2*;Cheng Zhuomin²;Wang Hongqing^1*

(¹Department of Fruit Science, College of Agronomy and Biotechnology, China Agricultural University, Beijing 100094, China;²State Key Laboratory of Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100094, China)

Received:2006-06-21 Revised:2006-08-21 Online:2006-12-25 Published:2006-12-25

Abstract

Abstract: Low molecular mass RNAs extracted from bark of one year old branch of 24 apricot samples, 37 plum samples were used for DIG-cDNA Dot-blot hybridization, RT-PCR, Return-PAGE and biological indexing. There were no clear symptoms when these samples were collected. The result showed that 3 of 24 apricot samples, 6 of 37 plum samples were infected with Hop stunt viroid (HSVd). a5, a17, p6, p16 selected from the 9 samples were used for cloning and sequencing analysis. HSVd sequence variants obtained were
compared with HSVd reported in GenBank and showed 99.0% - 99.7% identity. There was no clear relativity among sequence variants, region and host. These results suggest that sequence of HSVd is relatively conserved.

Key words: Hop stunt viroid, Detection, Apricot, Plum

CLC Number:

Yang Yuanai;;Li Shifang;Cheng Zhuomin;Wang Hongqing. Detection and Sequence Analysis of Hop stunt viroid in Apricot and Plum[J]. ACTA HORTICULTURAE SINICA, 2006, 33(6): 1193-1198.

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Detection and Sequence Analysis of Hop stunt viroid in Apricot and Plum

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