Cloning and Expression Characteristics of cDNA Fragment of GABAa Receptor Gene in Diamondback Moth ( Plutella xylostella )

ACTA HORTICULTURAE SINICA ›› 2006, Vol. 33 ›› Issue (2): 300-305.

• 研究论文 • Previous Articles Next Articles

Cloning and Expression Characteristics of cDNA Fragment of GABAa Receptor Gene in Diamondback Moth ( Plutella xylostella )

Zhou Xiaomao^{1, 2, 3};Wu Qingjun^1*;Hu Meiying²;Zhang Youjun¹;Zhu Guoren¹; Xu Baoyun¹

(¹Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China; ²Laboratory of Insect Toxicology, South China Agricultural University, Guangzhou 510642, China; ³Department of Pesticide Science, Hunan Agricultural University, Changsha 410128, China)

Received:2005-07-20 Revised:2005-10-09 Online:2006-04-25 Published:2006-04-25

Abstract

Abstract: One fragment of 248 bp cDNA of the GABAa recep tor gene from the fourth instar larvae of diamondback moth (DBM, Plutella xylostella) was amplified, cloned, sequenced and analyzed by using reverse transcription polymerase chain reaction (RT-PCR) method. Homologous analysis showed that it had 100% homologywith the published deduced amino acid sequences of Helioth is viresce. Based on the GABAa receptor gene clone, an optimal emiquantitative RT-PCR method was successfully constructed. Using 18S rRNA as internal control, the exp ression difference of GABAa recep tor gene in different tissues and different developmental stages was studied. Results showed that the gene was expressed the highest level in heads of DBM, next was in thorax and the lowest level in abdomen. The exp ression at different developmental stages was not significantly different.

Key words: Plutella xylostella, GABAa receptor gene, Semi-quantitative RTPCR, Gene expression

Zhou Xiaomao;;;Wu Qingjun;Hu Meiying;Zhang Youjun;Zhu Guoren;Xu Baoyun. Cloning and Expression Characteristics of cDNA Fragment of GABAa Receptor Gene in Diamondback Moth ( Plutella xylostella )[J]. ACTA HORTICULTURAE SINICA, 2006, 33(2): 300-305.

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Cloning and Expression Characteristics of cDNA Fragment of GABAa Receptor Gene in Diamondback Moth ( Plutella xylostella )

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