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ACTA HORTICULTURAE SINICA ›› 2006, Vol. 33 ›› Issue (2): 244-248.

• 研究论文 • Previous Articles     Next Articles

Analysis of Apple Fruit Acid /Low-acid Trait by SSR Marker

Yao Yuxin1;Zhai Heng1*;Zhao Lingling1; Yi Kai2;Liu Zhi2;Song Ye1

  

  1. (1College of Horticultural Science and Engineering, Shandong Agricultural University, Tai'an 271018, China; 2Liaoning Fruit Research Institute, Xiongyue 115214, China)
  • Received:2005-05-09 Revised:2005-08-30 Online:2006-04-25 Published:2006-04-25

Abstract:

It is necessary for app le genomic research and breeding to find outmalic acid genetic characteristics. In this research, SSR marker linked to acid /low-acid trait in app le fruitwas identified from 140 SSR primer pairs, materialed by 91 F1 population hybrid from the intraspecific cross between apple cultivar‘Dongguang’ and ‘Fuji’. Of 140 SSR primer pairs, only p rimer SDY085 produced a polymorphic band linked to acid trait, and the linkage distance was 8.89 cM. Also, the titrated acid and malic acid in different developmental stages were determined. The SSR marker analysis, coupled with the change of the total acid and malic acid contents, revealed that acid/low-acid trait was governed by a major gene and acid trait was complete dominant.

Key words: Apple, Malus ×domestica Borkh., Acid/low-acid trait, SSR marker, Malic acid