Isozyme Patterns and RAPD Analysis of Apple Plantlets Repea tedly Subcultured in Vitro

ACTA HORTICULTURAE SINICA ›› 2006, Vol. 33 ›› Issue (1): 33-37.

• 研究论文 • Previous Articles Next Articles

Isozyme Patterns and RAPD Analysis of Apple Plantlets Repea tedly Subcultured in Vitro

Du Guoqiang¹;Shi Xiaoxin¹;Zhang Qingliang²;Ma Baokun¹

(¹College of Horticulture, Agricultural University of Hebei, Baoding 071001, China; ²Cotton Research Institute, Hebei Academyof Agriculture and Forestry Sciences, Shijiazhuang 050051, China)

Received:2005-05-12 Revised:2005-09-13 Online:2006-02-25 Published:2006-02-25

Abstract

Abstract: The genetic stability of apple plantlets repeatedly subcultured in vitro for three to ninety times
was examined by analysis of isozyme patterns of POX, EST and AMY, aswell as RAPD. The results showed
that there were clear difference in POX, EST, and AMY isozyme patterns among varieties, but there was no
detetable difference in the same varietieswith different times of subculturing. The POX isozyme was further analyzed by using IEF electrophoresis. It was easy to distinguish between genotypes ( i.e. ‘Golden Delicious’
vs. ‘Jonagold’) according to the pI of POX isozyme. However, no difference was found in the same varieties
with different times of subculturing. RAPD analysiswith twenty-five 10-mer arbitrary primers revealed identical bands for plantlets with different times of subculturing within avariety. Our results indicated that genetic
stability of apple plantlets wasmaintained even after repeatedly subcultured in vitro for up to ninety times.

Key words: Apple, Tissue culture seedling, Genetic variation, Isozyme patterns, RAPD

Du Guoqiang;Shi Xiaoxin;Zhang Qingliang;Ma Baokun. Isozyme Patterns and RAPD Analysis of Apple Plantlets Repea tedly Subcultured in Vitro[J]. ACTA HORTICULTURAE SINICA, 2006, 33(1): 33-37.

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Isozyme Patterns and RAPD Analysis of Apple Plantlets Repea tedly Subcultured in Vitro

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