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ACTA HORTICULTURAE SINICA ›› 2005, Vol. 32 ›› Issue (4): 604-608.

• 研究论文 • Previous Articles     Next Articles

The RAPD and SSR Analysis of ‘Cabernet Shelongzhu’and Other Wine Grape Cultivars

Yao Yuxin1;Zuo Fangmei1;Zhai Heng1;Liu Laixin2   

  1. (1College of Horticultural Science and Engineering, Shandong Agricultural University, Tai'an 271018, China; 2 Yantai COFCO W inery, Penglai 265608, China)
  • Received:2004-12-27 Revised:2005-05-23 Online:2005-08-25 Published:2005-08-25

Abstract: ‘Cabernet Shelongzhu’ is an important grape cultivar in China used for wine. However, its genetic origin is not clear yet. In this study, depending on the RAPD (Random Amp lification Polymorphism DNA) analysis with 36 primers, 14 wine grape cultivars were classified into four groups by UPGMA ( unweighted pair group method using arithmetic average) cluster analysis. Four cultivars of them, i. e. Cabernet Sauvignon, Cabernet Shelongzhu, Cabernet Franc and Merlot, belonged to the same group. The SSR ( Simple Sequence Repeat) analysis further indicated that they were four independent cultivars. Also, the results from SSR analysis showed that three phenotypes of‘Cabernet Shelongzhu’ sampled from different regions were genetically distinct with Euclidean distances ranging from 1.41 to 1.73, which were much smaller than those among distinct cultivars. Comparingwith other cultivars, the phenotype of Cabernet Shelongzhu from Beigou at Penglai was the closest to Cabernet Franc with the Euclidean distance of 2.45, and followed by Changli and
Yantai with the Euclidean distances of 2.56 and 2.64, respectively. The historical origin of the cultivar ‘Cabernet Shelongzhu’was investigated and discussed.

Key words: Grape, RAPD, SSR, Cluster analysis, Historical origin

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