Cloning and Sequence Analysis of Thioredoxin-h-like THL2 Gene in Brassica napus L. and Brassica olereacea L．

ACTA HORTICULTURAE SINICA ›› 2004, Vol. 31 ›› Issue (3): 381-383.

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Cloning and Sequence Analysis of Thioredoxin-h-like THL2 Gene in Brassica napus L. and Brassica olereacea L．

Wang Maoguang¹ ;Wu Junyan¹ ;Wu Nengbiao² ;Zhu Liquan^1*;Wang Xiaojia^2*

(¹Laboratory ofBiochemistry，College ofAgronomy and Life Sciences，Southwest Agricultural University，Chongqing 400716，China； ²Chongqing Key Laboratory of Olericulture，College of Horticulture and Gardens，Southwest Agricultural University，Chongqing 400716．China)

Received:2003-07-15 Revised:2003-10-14 Online:2004-06-25 Published:2004-06-25

Abstract

Abstract: Thioredoxin-h-like protein DNA and c DNA were amplified from genomic DNA and stigma total RNA in Brassica napus L．and B．olereacea L. by PCR and RT-PCR method respectively．Sequence analysis first indicated THL2 gene contained two introns．which were diferent in size between B．napus L. and B．olereacea L. Homologous analysis demonstrated that there was 96％ identity of DNA from B.napus L. and B．olereacea L．．

Key words: Self-incompatibility, THL2 gene, Cloning, Sequence analysis

CLC Number:

s635

Wang Maoguang;Wu Junyan;Wu Nengbiao;Zhu Liquan;Wang Xiaojia. Cloning and Sequence Analysis of Thioredoxin-h-like THL2 Gene in Brassica napus L. and Brassica olereacea L．[J]. ACTA HORTICULTURAE SINICA, 2004, 31(3): 381-383.

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Cloning and Sequence Analysis of Thioredoxin-h-like THL2 Gene in Brassica napus L. and Brassica olereacea L．

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