Acta Horticulturae Sinica ›› 2022, Vol. 49 ›› Issue (5): 1111-1120.doi: 10.16420/j.issn.0513-353x.2021-0260

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Identification of S-genotypes of 63 Pummelo Germplasm Resources

WEI Zhuangmin1,4, WEI Sijia1, CHEN Peng2, HU Jianbing1, TANG Yuqing3, YE Junli1, LI Xianxin2, DENG Xiuxin1, CHAI Lijun1,**()   

  1. 1Key Laboratory of Horticultural Plant Biology(Ministry of Education),Huazhong Agricultural University,Wuhan 430070,China
    2Institute of Horticulture,Hunan Academy of Agricultural Sciences,Changsha 410125,China
    3Institute of Horticulture,Jiangxi Academy of Agricultural Sciences,Nanchang 330200,China
    4Guangxi Subtropical Crops Research Institute,Nanning 530001,China
  • Received:2022-01-19 Revised:2022-02-05 Online:2022-05-25 Published:2022-05-25
  • Contact: CHAI Lijun


Based on 63 pummelo germplasm resources,new S-RNases were identified by RNA-seq and sequence analysis. Twenty-one pairs of specific primers were designed to amplify and identify the S-genotypes of 63 pummelo germplasm resources. Results showed twelve new S-RNases were found,named S10-RNase-S21-RNase in sequence. Specific amplification showed that 59 out of 63 materials were identified with complete S-genotype,only one S-RNase gene was identified in four materials. Among them,S2-RNase was amplified in 22 materials with the highest frequency of 34.92%(22/63),while S4-RNase and S6-RNase were not amplified in the 63 materials. The results also showed that many varieties had the same S-genotype.

Key words: Citrus, S-genotypes, self-incompatibility, specific primer

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