Acta Horticulturae Sinica ›› 2022, Vol. 49 ›› Issue (4): 769-777.doi: 10.16420/j.issn.0513-353x.2021-0329

• Research Papers • Previous Articles     Next Articles

Comparison of Sterilization Methods for Kiwifruit Pollen and Its Effect on Fruit Quality

LI Li, FENG Dandan, PAN Hui, LI Wenyi, DENG Lei, WANG Zupeng, ZHONG Caihong*()   

  1. CAS Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture,CAS Engineering Laboratory for Kiwifruit Industrial Technology,Wuhan Botanical Garden,Chinese Academy of Sciences,Wuhan 430074,China
  • Received:2021-11-11 Revised:2022-03-15 Online:2022-04-25 Published:2022-04-24
  • Contact: ZHONG Caihong


Pollen has been proved to be one of the main pathways for long-distance transmission of kiwifruit bacterial canker(Pseudomonas syringae pv. actinidiae,Psa). Five physical sterilize methods including 60Co-γ irradiation,ozone,UV,microwave,water-bath heating(35,50,80,100 ℃),and eight chemical sterilize methods including ClO2,ethylicin,tetramycin,kasugamycin,cuprous oxide,amobam,zhongshengmycin and sliver nanoparticles were applied to analyze the effect to kiwifruit pollen. The index of Psa sterilization rate,pollen viability,fruit setting rate,single fruit weight and fruit quality were analyzed. The results showed that the sterilization rate and pollen viability of partial treatments are relatively low,such as UV,microwave,water-bath heating,ethylicin,tetramycin,kasugamycin and amobam solution. Other treatments caused the index including fruit setting rate,single fruit weight,size,fruit firmness,soluble solids,dry matter,vitamin C,soluble sugar and titratable acid content decreased significantly,such as 60Co-γ irradiation,ClO2,cuprous oxide,Zhongshengmycin and sliver nanoparticles. The optimum disinfection method is ozone with concentration of 642.85 mg · m-3 for 1 h,the Psa sterilization rate reached 99.52% while the decline rate of pollen viability was merely declined 2.92%. The fruit setting rate,fruit firmness,vitamin C,soluble sugar and titratable acid,single fruit weight,size,dry matter and soluble solids index decreased slightly without significant difference. Comprehensive analysis the cost,operability and environmental protection factors,ozone technology can be regarded as the most effective and safe method for kiwifruit pollen sterilization.

Key words: kiwifruit, bacterial canker, pollen, sterilization method, sterilization rate, viability, fruit quality

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