Acta Horticulturae Sinica ›› 2022, Vol. 49 ›› Issue (3): 581-589.doi: 10.16420/j.issn.0513-353x.2020-0815

• Research Papers • Previous Articles     Next Articles

High Temperature Treatment Generates Unreduced Pollen in Paeonia suffruticosa

CHENG Shiping*(), YAO Pengqiang, GENG Xining, LIU Chunyang, XIE Lihua   

  1. Henan Province Key Laboratory of Germplasm Innovation and Utilization of Eco-economic Woody Plant,School of Chemistry and Environmental Engineering,Pingdingshan University,Pingdingshan,Henan 467000,China
  • Received:2021-06-22 Revised:2022-01-24 Online:2022-03-25 Published:2022-03-25
  • Contact: CHENG Shiping


In this research,five-year Paeonia suffruticosa‘Fengdan’was chosen as an experimental material. On the basis of mastering meiosis process of pollen mother cells,“a non-in-vitro branch bud heat treatment device of trees”(ZL200610113448.X)was used to implement pollen chromosome doubling of P. suffruticosa under high-temperature processing,a suitable technique for high temperature-induced pollen chromosome doubling in P. suffruticosa was established. The findings indicated that meiosis process of pollen mother cells had correlation with the external form of flower buds and anther color changes. When flower buds remained between“small wind bell”stage to“big wind bell”stage,namely the diameter of flower buds was 13 mm,meiosis process of pollen mother cells reached diplonema—metaphaseⅠ. The treatment combination of 40 ℃ for 4 h produced the greatest amount of diploid pollen(up to 21.98% of the total yield).

Key words: Paeonia suffruticosa, triploid breeding, meiosis process, 2n pollen, high temperature

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