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Acta Horticulturae Sinica ›› 2021, Vol. 48 ›› Issue (2): 313-324.doi: 10.16420/j.issn.0513-353x.2020-0232

• Research Papers • Previous Articles     Next Articles

Cloning and Characterization of Key Synthase FAS Gene Involved in Terpenoids Pathway of Chrysanthemum morifolium

HU Hao1,2, YANG Ting3, GAO Liping3, Maarten A. Jongsma3, WANG Caiyun1,2,*()   

  1. 1Key Laboratory for Biology of Horticultural Plants,Ministry of Education,Huazhong Agricultural University,Wuhan 430070,China
    2Key Laboratory of Urban Agriculture in Central China,Ministry of Agriculture and Rural Affairs,P. R. China,Wuhan 430070,China
    3Wageningen Plant Research,Wageningen University and Research,P.O. Box 619,6700 AP,Wageningen,The Netherlands
  • Received:2020-09-22 Revised:2020-12-07 Online:2021-02-25 Published:2021-03-09
  • Contact: WANG Caiyun E-mail:wangcy@mail.hzau.edu.cn

Abstract:

The secondary metabolite composition and relative abundance in leaves and ovaries of the Chrysanthemum morifolium model cultivar‘1581’were analyzed by SPME extraction and GC-MS analysis. The terpenoids represented the dominant group of volatiles. Based on conserved sequences FDS-like genes identified in a transcriptome database derived from chrysanthemum,a farnesol synthase gene(CmFAS)was cloned with an 1 197 bp open reading frame(ORF),encoding 398 amino acids and putatively located in the plastids. Phylogenetic analysis showed that CmFAS belongs to the FDS gene family in Asteraceae and has higher sequence similarity with the monoterpene CDS genes of pyrethrum (Tanacetum cinerariifolium)and Artemisia. Multiple alignment of FDS proteins of several different plant species demonstrated that CmFAS had the five typical conserved regions of chain elongation prenyltransferases. The gene expression of CmFAS was the highest in young leaves and lower in ovaries and mature leaves. The expression level of CmFAS was lower than that of CmFDS1 and CmFDS2 in chrysanthemum. By enzyme assay,it showed that CmFAS not only catalyzed DMAPP and IPP to farnesol but also converted CPP to chrysanthemol. CmFAS is a bifunctional enzyme generating both monoterpene and sesquiterpene products. CmFAS is the first sesquiterpene synthase located in plastids of chrysanthemum.

Key words: Chrysanthemum, farnesol synthase, terpenoids, gene expression, prokaryotic expression

CLC Number: