Abstract: Laccase has been shown a multifunctional enzyme that plays many important roles. The whole genome-wide of identification of banana laccase family members was conducted and analyzed. Using bioinformatics approaches，22 laccase genes（MaLAC）were identified from Musa acuminata genome and 11 laccase genes（MbLAC）were also identified from Musa balbisiana genome. Most of MaLAC and MbLAC members contained three typical Cu-oxidase domains. Gene structure analysis showed that most of MaLACs and MbLACs were composed of five introns and six exons. Phylogenetic clustering analysis indicated that the MaLACs were divided into five groups and the MbLACs were divided into four groups. The promoter cis-acting elements showed that the MaLAC promoter sequence contained a large number of stress response elements，such as light response，hormone response and abiotic stress，etc. The results of qRT-PCR analysis showed that the expression of some members of MaLACs increased under low temperature stress. The expression of MaLAC3-2，MaLAC4，MaLAC4-5，and MaLAC17 increased significantly at low temperatures of 13 ℃，4 ℃ and 0 ℃. Moreover，18 MaLAC transcripts were found to be potential targets of miR397 or miR408.

Key words: banana, Laccase family, member identification, bioinformatics analysis, low temperature stress