Studies on the Regulation of Anthocyanin Accumulation by Apple Sucrose Transporter MdSUT2

doi:10.16420/j.issn.0513-353x.2018-0501

ACTA HORTICULTURAE SINICA ›› 2019, Vol. 46 ›› Issue (1): 1-10.doi: 10.16420/j.issn.0513-353x.2018-0501

• Research Papers • Next Articles

Studies on the Regulation of Anthocyanin Accumulation by Apple Sucrose Transporter MdSUT2

LU Jing，MA Qijun，LIU Xiao，KANG Hui，LIU Yajing，HAO Yujin，and YOU Chunxiang*

（State Key Laboratory of Crop Biology，MOA Key Laboratory of Horticultural Crop Biology（Huanghuai Region）and Germplasm Innovation，College of Horticulture Science and Engineering，Shandong Agricultural University，Tai’an，Shandong 271018，China）

Online:2019-01-25 Published:2019-01-25

Abstract

Abstract:

A gene named MdSUT2（MDP0000277235）was cloned from‘Gala’apple（Malus × domestica Borkh.）. Sequence analysis showed that this gene contained 1 704 bp nucleotides，and encoded a protein of 566 amino acids. Overexpression vector of MdSUT2 was constructed and transformed into Arabidopsis seedlings via Agrobacterium-mediated method. Semi RT-PCR analysis indicated that MdSUT2 was genetically transformed into Arabidopsis. In addition，the transient expression vectors were constructed and transformed into apple fruits. We found that overexpression of MdSUT2 in apple fruits significantly increased the anthocyanin content，whereas suppression of MdSUT2 decreased the anthocyanin content.

Key words: apple, MdSUT2, gene clone, functional identification, anthocyanin

CLC Number:

S 661.1

LU Jing，MA Qijun，LIU Xiao，KANG Hui，LIU Yajing，HAO Yujin，and YOU Chunxiang*. Studies on the Regulation of Anthocyanin Accumulation by Apple Sucrose Transporter MdSUT2[J]. ACTA HORTICULTURAE SINICA, 2019, 46(1): 1-10.

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Studies on the Regulation of Anthocyanin Accumulation by Apple Sucrose Transporter MdSUT2

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