Abstract: Two apple test materials 1-1-24 and 1-2-34 generated from hybrids of‘Jiguan’&‘Fuji’ were chosen as experimental materials. 1-1-24 was resistant to ring rot disease while 1-2-34 was highly susceptible. Each needle was stabbed on both sides of main vein of the blade. Culture medium cakes covered with Botryosphaeria berengeriana f. sp. piricola and sterile distilled water were separately inoculated to different blades. Total protein of apple leaves before treatment and after 24 h of treatment were suitable for the analysis of 2-DE and identified by mass spectrometry. There were 27 proteins successfully identified. During the process of apple leaves response to the ring rot pathogen stress，multiple pathways were pulsed on to achieve to stress related，defense related，cellular metabolism，synthesis related，photosynthesis and unknown. Combined with real-time quantitative PCR analysis of Glucanendo-1,3-beta-glucosidase，AP15 and acidic endochitinase，we came to the conclusion that Glucanendo-1,3-beta-glucosidase and acidic endochitinase played an important role in resistance to B.berengeriana f. sp. piricola and the key proteins’ extremely significant difference in content without pathogen infection probably was one of the reasons for the resistance difference.

Key words: apple, Botryosphaeria berengeriana f. sp. Piricola, proteomic, key protein