Identification of Kolra Fragrant Pear Calyx-end Black Spot Pathogen and Its Sequence Analysis of ITS，GPD and EF-1α

doi:10.16420/j.issn.0513-353x.2015-0879

ACTA HORTICULTURAE SINICA ›› 2016, Vol. 43 ›› Issue (2): 329-336.doi: 10.16420/j.issn.0513-353x.2015-0879

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Identification of Kolra Fragrant Pear Calyx-end Black Spot Pathogen and Its Sequence Analysis of ITS，GPD and EF-1α

SONG Bo，ZHU Xiao-feng，XU Bing-qiang，Abudu Keyimu ? Kader，and YANG Sen*

Institute of Plant Protection，Xinjiang Academy of Agricultural Sciences，National Local Joint Engineering Research Center of Special Forestry and Fruit Industry；Key Laboratory of Integrated Pest Management on Crops in Northwestern Oasis Ministry of Agriculture，Urumqi 830091，China

Online:2016-02-25 Published:2016-02-25

Abstract

Abstract: A study was conducted to definitude the disease pathogen and its taxonomic status. The pathogen was isolated from disease tissue samples of Kolra Fragrant pear calyx-end black spot with conventional tissue isolation. The pathogenicity test was conducted and confirmed by Koch’s postulates. The pathogen was identified based on morphology and molecular identification. Determination of pathogenic field confirmed that the Alternaria sp. as the pathogen，and meanwhile，the general primer of fungal conserved genes（ITS，GPD and EF-1α）was taken advantage of for PCR amplification. After that，it was cloned and sequenced，and got the GenBank accession number：KU145269，KU145271，KU145273. Confirmed Alternaria alternata as the causative agent of Kolra Fragrant pear calyx-end black spot disease using morphological analysis，pathogenicity test and phylogenetic analysis of conserved genes.

Key words: pear, black spot, identification, Alternaria alternata, conserved genes

CLC Number:

S 661.2

SONG Bo，ZHU Xiao-feng，XU Bing-qiang，Abudu Keyimu ? Kader，and YANG Sen*. Identification of Kolra Fragrant Pear Calyx-end Black Spot Pathogen and Its Sequence Analysis of ITS，GPD and EF-1α[J]. ACTA HORTICULTURAE SINICA, 2016, 43(2): 329-336.

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Identification of Kolra Fragrant Pear Calyx-end Black Spot Pathogen and Its Sequence Analysis of ITS，GPD and EF-1α

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