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ACTA HORTICULTURAE SINICA ›› 2015, Vol. 42 ›› Issue (10): 1869-1878.doi: 10.16420/j.issn.0513-353x.2015-0126

• Fruit Trees •     Next Articles

Differential Expression Analysis in Fruit Softening and Ethylene Biosynthetic Pathways in Peaches of Different Flesh Textures

YANG Yong1,2,MA Rui-juan1,ZHANG Bin-bin1,SONG Zhi-zhong1,ZHANG Chun-hua1,Guo Shao-lei1,and YU Ming-liang1,2,*   

  1. 1Institute of Horticulture,Jiangsu Academy of Agricultural Sciences,Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement,Nanjing 210014,China;2College of Horticulture,Nanjing Agricultural University,Nanjing 210095,China
  • Online:2015-10-25 Published:2015-10-25

Abstract:

To study the expression difference of genes involved in fruit softening and ethylene biosynthetic pathways under different temperature storage,different peach cultivars of‘Xiacui’and‘Yumyeong’with stonyhard,‘Yinhualu’with soft-melting,‘Hujing Milu’with hard-melting,and‘Babygold 6’with non-melting in 8 tenth maturity were collected as test materials. The results showed that only a little ethylene production were detected after harvest with fruits of‘Yumyeong’and‘Xiacui’,under either room temperature(25 ℃)or low temperature(4 ℃). The fruit firmness of stonyhard cultivars retain at high level under room temperature with time going on,whereas low temperature induced‘Yumyeong’fruit to soften. Real-time quantitative PCR results indicated that PpACS1 gene was highly expressed in soft-melting,hard-melting and non-melting cultivars,but extremely low in stonyhard peaches. However,PpACS2 or PpACS3 could not be detected in all five cultivars. Moreover,cold treatment induced the expression level of PpACO1 gene in‘Xiacui’and‘Yumyeong’peaches,and significantly enhanced endo-PG expression in‘Yumyeong’peaches. In conclusion,this study revealed that fruit softening of peaches with different flesh textures were closely related to ethylene biosynthesis during the storage period,which were controlled via regulating relevant gene expression levels under different storage temperatures.

Key words: Prunus persica, ethylene, biosynthetic pathway, soften, gene expression

CLC Number: