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Acta Horticulturae Sinica ›› 2022, Vol. 49 ›› Issue (6): 1313-1326.doi: 10.16420/j.issn.0513-353x.2021-0478

• Research Papers • Previous Articles     Next Articles

The Negative Regulation of DcERF-1 on Senescence of Cut Carnation

WANG Yan1,2, SUN Zheng1,2, FENG Shan1,2, YUAN Xinyi1,2, ZHONG Linlin1,2, ZENG Yunliu1,2, FU Xiaopeng1,4, CHENG Yunjiang1,2,3, Bao Manzhu1,4, ZHANG Fan1,2,3,4,*()   

  1. 1Key Laboratory of Horticultural Plant Biology,Ministry of Education,College of Horticulture and Forestry Sciences,Huazhong Agricultural University,Wuhan 430070,China
    2National R & D Center For Citrus Preservation,Wuhan 430070,China
    3Hubei Hongshan Laboratory,Wuhan 430070,China
    4Key Laboratory of Huazhong Urban Agriculture,Ministry of Agriculture and Rural Affairs,College of Horticulture and Forestry Sciences,Huazhong Agricultural University,Wuhan 430070,China
  • Received:2021-12-31 Revised:2022-04-22 Online:2022-06-25 Published:2022-07-05
  • Contact: ZHANG Fan E-mail:zhangfan@mail.hzau.edu.cn

Abstract:

In order to explore the mechanism of ethylene regulated flower senescence in cut carnation,we screened a transcription factor named DcERF-1 from the expression profile of carnation. Analysis of deduced amino acid sequence and phylogenetic tree found that DcERF-1 showed high homology with Arabidopsis thaliana AtERF-1,and belongs to Ⅸ(B3)sub-group. Real-time quantitative PCR showed that DcERF-1 was highly expressed in flower,and the expression trend of DcERF-1 was firstly increased and then decreased during both the natural flower senescence and ethylene induced flower senescence progress. The subcellular localization assay showed that DcERF-1 was localized in the nucleus. Transient overexpression of DcERF-1 in the petals resulted that the petal fading rate was significantly delayed and the ion leakage rate was significantly reduced. After transient silencing of DcERF-1,the petal fading rate was significantly accelerated,the ion leakage rate was significantly increased,and the expression of senescence marker gene DcSAG12 was significantly up-regulated. In addition,yeast one hybrid assay showed that DcEIN3 could directly bind the promoter of DcERF-1,and dual luciferase assay showed that DcERF-1 could inhibit the expression of DcACO4. The comprehensive results indicated that DcERF-1 negatively regulates the flower senescence in cut carnation.

Key words: carnation, cut flower senescence, ethylene, postharvest, ERF transcription factor, transcriptional regulation

CLC Number: