Abstract: Sixteen pairs SSR primers that evenly distributed on the 8 chromosome linkage group were used to analyze the genetic diversity of 24 Prunus salicina varieties from different producing areas. The result showed that the PIC of each primer was ranging from 0.547 to 0.783，with the lowest primer CPSCT005 and the maximum primer CPSCT022. The effective alleles，Nei’s gene diversity and Shannon’s information of different primers differed significantly，in which primer CPSCT031 was the lowest while primer CPSCT039 was the maximum. According to the analysis of all primers，the relationship of effective alleles，Nei’s gene diversity and Shannon’s information of three producing areas were the same：Northern variety and southern variety were less different and greater than foreign variety. The populations of southern variety and foreign variety were clustered together. Sixteen pairs primers expanded 86 sites in total，while the polymorphic loci were 81 with the percentage of 94.19%，and the amplification loci of each pair of primers amplification in average was 5.38. Cluster results showed that in line 0.35，24 species were divided into two groups，and most of the northern varieties gathered for a class whereas the southern varieties and foreign varieties gathered into a group. The results indicated in molecular level that foreignvarieties originated from China.

Key words: Prunus salicina, SSR, primer efficiency, populations, genetic diversity