园艺学报 ›› 2016, Vol. 43 ›› Issue (7): 1402-1410.doi: 10.16420/j.issn.0513-353x.2015-0872

• 研究报告 • 上一篇    下一篇


杨 哲1,刘克林2,彭佳佳1,3,秦 扬1,3,郑高言1,明 军1,刘 春1,*   

  1. (1中国农业科学院蔬菜花卉研究所,北京 100081:2北京市大东流苗圃,北京 102211;3宁夏大学农学院,银川 750021)
  • 出版日期:2016-07-25 发布日期:2016-07-25

Cloning and Expression Analysis of Chalcone Isomerase Gene from Anthurium andraeanum

YANG Zhe1,LIU Ke-lin2,PENG Jia-jia1,3,QIN Yang1,3,ZHENG Gao-yan1,MING Jun1,and LIU Chun1,*   

  1. (1Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Beijing 100081,China;2Nursery of Dadongliu,Beijing 102211,China;3College of Agricuture,Ningxia University,Yinchuan 750021,China)
  • Online:2016-07-25 Published:2016-07-25


以红掌栽培品种‘Dakota’为材料,采用RACE技术克隆获得AnCHI基因的cDNA序列,分析花青素含量与该基因表达的关系,进一步完善红掌花青素合成途径。结果表明:AnCHI序列全长为1 117 bp,其中开放阅读框为777 bp,编码258个氨基酸;AnCHI在不同时期红掌佛焰苞以及根、茎、叶、肉穗组织中均有表达,且在佛焰苞发育前期表达量明显高于后期,测得花青素含量与基因表达量变化基本一致;构建的pGEX-4T1-AnCHI重组表达载体在原核中正确表达。初步验证了AnCHI的表达可能对红掌佛焰苞花青素的合成有一定促进作用。

关键词: 红掌, AnCHI, 花青素, 表达分析


A full length cDNA of chalcone isomerase(CHI)was amplified from Anthurium andraeanum L.‘Dakota’by using RACE technology. The relationship between its expression patterns and anthocyanin content were dissected for the purpose of further improving the anthocyanin biosynthetic pathway of anthurium. The results showed that the AnCHI cDNA was 1 117 bp with a 777 bp open reading frame in full-length which encoding 258 amino acids. AnCHI was expressed in different developing periods of spathes as well as in roots,stems,leaves and spadixes and the expression levels of AnCHI in the early stages(1–4)of spathes were significantly higher than that in mature stages(5–8). The content of anthocyanin was changed consistently with the gene expression,while the recombinant expression vectors—pGEX-4T1-AnCHI was correctly expressed in prokaryotes. The research suggested that AnCHI may play an important role in promoting the anthocyanin synthesis of Anthurium andraeanum.

Key words: Anthurium andraeanum, AnCHI, anthocyanin, expression analysis