园艺学报 ›› 2022, Vol. 49 ›› Issue (9): 1935-1944.doi: 10.16420/j.issn.0513-353x.2021-0638

• 研究论文 • 上一篇    下一篇


黄玲, 胡先梅, 梁泽慧, 王艳平, 产祝龙*(), 向林*()   

  1. 华中农业大学园艺林学学院,园艺植物生物学教育部重点实验室,武汉 430070
  • 收稿日期:2021-08-16 修回日期:2021-10-13 出版日期:2022-09-25 发布日期:2022-10-08
  • 通讯作者: 产祝龙,向林;
  • 基金资助:

Cloning and Function Identification of Anthocyanidin Synthase Gene TgANS in Tulipa gesneriana

HUANG Ling, HU Xianmei, LIANG Zehui, WANG Yanping, CHAN Zhulong*(), XIANG Lin*()   

  1. Key Laboratory of Horticultural Plant Biology,Ministry of Education,College of Horticulture and Forestry Sciences,Huazhong Agricultural University,Wuhan 430070,China
  • Received:2021-08-16 Revised:2021-10-13 Online:2022-09-25 Published:2022-10-08
  • Contact: CHAN Zhulong,XIANG Lin;


以红色的郁金香品种‘纯火’为材料,采用高效液相色谱技术对其4个花期的花青素苷含量进行了测定,结果表明,随着花朵的发育,花青素苷的含量逐渐升高,在盛开期含量最高,达3 002.75 μg · g-1 FW。从花中克隆出结构基因TgANS,GenBank登录号为MZ442383,开放阅读框长1 071 bp,编码356个氨基酸,TgANS基因表达水平在不同花期存在显著差异,随着花发育逐渐升高,表达趋势与花青素苷的积累趋势基本一致。将TgANS导入烟草进行异源表达,转基因株系花色明显变红,TgANS表达量显著增加,花青素苷含量增加,进一步对这些株系内源的NtCHS、NtCHINtFLS、NtF3H、NtF3’HNtDFR表达进行分析,NtF3H、NtF3’HNtDFR表达量显著上升,NtCHSNtCHI表达量下降。表明郁金香TgANS参与了花青素苷的合成。

关键词: 郁金香, 花青素合成酶(ANS), 花青素苷, 基因功能


In this study,the contents of anthocyanins in the tepals were determined from flower of red Tulipa gesneriana‘Strong Fire’at four developmental stages by high performance liquid chromatography(HPLC). The results showed that the content of anthocyanins continued to increase along with the development of flowers,and the highest content was up to 3 002.75 μg · g-1 FW in fully flowering stage flower. The sequence of TgANS was cloned,and the GenBank accession number was MZ442383. TgANS has 1 071 bp ORF region,encoding 356 amino acids. The expression level of TgANS differed significantly at different flower stages,and showed a trend of rising during the flower development. The expression pattern was consistent with the accumulation of anthocyanin in the four stages of flower development. Ectopic expression of TgANS in transgenic tobacco plants showed that the flowers of transgenic plants obviously turned red and TgANS was expressed at high levels in transgenic plants. The anthocyanin content in the tepals of the transgenic plants was slightly higher than that in empty vector control. Further analysis of the flower color related genes indicated that the expression of genes NtF3H,NtF3’H and NtDFR were increased significantly,and NtCHS,NtCHI were decreased in transgenic tobacco plants. These results indicated that tulip TgANS gene is involved in the biosynthesis of anthocyanin.

Key words: Tulipa gesneriana, anthocyanidin synthase(ANS), anthocyanin, gene function