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https://www.ahs.ac.cn/images/0513-353X/images/top-banner2.jpg|#|甘蓝
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https://www.ahs.ac.cn/images/0513-353X/images/top-banner12.jpg|#|菊花

园艺学报 ›› 2022, Vol. 49 ›› Issue (6): 1301-1312.doi: 10.16420/j.issn.0513-353x.2021-0629

• 研究论文 • 上一篇    下一篇

白菜、甘蓝和甘蓝型油菜PAP1/2同源基因的鉴定及分析

陈道宗, 刘镒, 沈文杰, 朱博, 谭晨()   

  1. 赣南师范大学生命科学学院,赣州市设施蔬菜重点实验室,江西赣州 341000
  • 收稿日期:2021-06-29 修回日期:2021-11-27 出版日期:2022-06-25 发布日期:2022-07-05
  • 通讯作者: 谭晨 E-mail:tanchen2020@gnnu.edu.cn
  • 基金资助:
    江西省自然科学基金项目(20212BAB215002);国家自然科学基金项目(32160454);赣州市科技计划项目(赣市科发[2020]60号)

Identification and Analysis of PAP1/2 Homologous Genes in Brassica rapa,B. oleracea and B. napus

CHEN Daozong, LIU Yi, SHEN Wenjie, ZHU Bo, TAN Chen()   

  1. College of Life Sciences,Ganzhou Key Laboratory of Greenhouse Vegetable,Gannan Normal University,Ganzhou,Jiangxi 341000,China
  • Received:2021-06-29 Revised:2021-11-27 Online:2022-06-25 Published:2022-07-05
  • Contact: TAN Chen E-mail:tanchen2020@gnnu.edu.cn

摘要:

PAP1/2转录因子是参与形成MBW复合体从而调控花青素合成的主要MYB转录因子。以拟南芥PAP1/2转录因子核酸和蛋白序列比对及Pfam验证,研究白菜、甘蓝和甘蓝型油菜等芸薹属植物中PAP1/2转录因子调控花青素代谢的确切同源拷贝数、进化关系及表达情况:在白菜、甘蓝及甘蓝型油菜中鉴定到的PAP1/2转录因子同源拷贝数分别为3、4和9个;通过进化分析和PAP1/2转录因子同源拷贝所在区段微共线性分析进一步明确了其不同拷贝间的进化关系,厘清了A7和C6染色体上PAP1/2转录因子不同拷贝的相互关系;结合白菜、甘蓝和甘蓝型油菜不同组织转录组测序数据进行表达情况分析,阐明了PAP1/2转录因子同源拷贝在不同组织中的表达情况。

关键词: 白菜, 甘蓝, 甘蓝型油菜, 同源基因, 保守结构域, 花青素, R2R3-MYB转录因子, PAP1/2

Abstract:

PAP1/2 transcription factors are the main MYB transcription factors involved in the formation of MBW complex to regulate anthocyanin biosynthesis. Using Arabidopsis PAP1/2 transcription factor(TF)nucleic acid and protein sequence alignment and Pfam verification to study the exact homologous copy number,evolutionary relationship and expression of PAP1/2 TFs regulating anthocyanin metabolism in Brassica plants such as Brassica rapa,B. oleracea and B. napus. The number of homologous copies of PAP1/2 TFs identified in B. rapa,B. oleracea and B. napus were three,four and nine,respectively. The evolutionary relationship between different copies of PAP1/2 TFs was further clarified by evolution analysis and microcollinearity analysis of the segment where the homologous copies of PAP1/2 TFs were located. The relationship between different copies of PAP1/2 TFs on chromosomes A7 and C6 was clarified. Finally,combined with the transcriptome sequencing data of different tissues of B. rapa,B. oleracea and B. napus,the expression analysis was carried out,and the expression of homologous copies of PAP1/2TFs in different tissues was clarified.

Key words: Brassica rapa, B. Oleracea, B. Napus, homologous gene, conserved domain, anthocyanin, R2R3-MYB transcription factor, PAP1/2

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