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园艺学报 ›› 2010, Vol. 37 ›› Issue (8): 1325-1328.

• 研究报告 • 上一篇    下一篇

梨果实褐皮性状的SSR标记

宋 伟,王彩虹*,田义轲,田 伟,殷 豪   

  1. (青岛农业大学园艺学院,山东青岛 266109)
  • 收稿日期:2010-03-16 修回日期:2010-06-23 出版日期:2010-08-25 发布日期:2010-08-25
  • 通讯作者: 王彩虹

SSR Molecular Markers Linked to the Fruit Russet Skin of Pear

SONG Wei,WANG Cai-hong*,TIAN Yi-ke,TIAN Wei,and YIN Hao   

  1. (College of Horticulture,Qingdao Agricultural University,Qingdao,Shandong 266109,China)
  • Received:2010-03-16 Revised:2010-06-23 Online:2010-08-25 Published:2010-08-25
  • Contact: WANG Cai-hong

摘要: 以梨品种‘黄金’(Pyrus pyrifolia Nakai‘Whangkeumbae’)与‘砀山酥’(Pyrus bretschneideri Rehd.‘Dangshansu’)的F1代杂交分离群体为试材,用分离群体分组分析法(Bulked Segregant Analysis,BSA)对果实褐皮性状进行了SSR分子标记研究。通过对源自梨和苹果基因组的281对SSR引物的筛选,获得了与梨果实褐皮性状相连锁的SSR标记CH01c06和Hi20b03,遗传连锁距离分别为4.8 cM和12.0 cM,据此,将控制该性状的基因定位在梨公共遗传图谱的LG8上。

关键词: 梨, 褐皮性状, SSR标记, 基因定位

Abstract: With the progenies produced from the cross Pyrus pyrifolia Nakai ‘Whangkeumbae’× Pyrus bretschneideri Rehd.‘Dangshansu’,the method of bulked segregant analysis (BSA) was used to identify molecular markers linked to the trait of fruit russet skin in pear. Two hundred and eighty one pairs of SSR primers derived from pear and apple genome were screened. Two SSR markers,CH01c06 and Hi20b03,which linked to russet skin of fruit at a distance of 4.8 cM and 12.0 cM,respectively,were obtained. This suggests that the gene which determines fruit russet skin be located on LG8 of the pear consensus genetic map.

Key words: pear, russet skin, SSR marker, gene localization

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