园艺学报 ›› 2004, Vol. 31 ›› Issue (2): 256-258.

• 研究报告 • 上一篇    下一篇


汪 琼;王 燕;程水源;费永俊;金卫斌

  1. ( 扬州大学园艺系,扬州 225009; 湖北农学院园艺系,荆州 434025; 湖北农学院湖北省涝渍灾害与湿地农业重点实验室,荆州 434025)
  • 收稿日期:2003-04-24 修回日期:2003-07-28 出版日期:2004-04-25 发布日期:2004-04-25

Molecular Cloning and Sequence of Acid Invertase Gene Fragments(CMCW1) from Clivia miniata

Wang Qiong;Wang Yan;Cheng Shuiyuan;Fei Yongjun;Jin Weibin

  1. ( Department of Horticulture,Yangzhou University,Yangzhou 225009,China;Department of Horticulture,Hubei Agricultural College,Jingzhou 434025,China;Key Laboratory of Waterlogged Disaster and Wetland Agriculture in Hubei Province,Jingzhou 434025,China)
  • Received:2003-04-24 Revised:2003-07-28 Online:2004-04-25 Published:2004-04-25

摘要: 分析植物酸性转化酶基因的保守区序列,设计一对PCR引物,以君子兰基因组DNA为模板,
采用PCR方法扩增出长约500 bp的DNA片段,克隆入pGEM-TEasy载体,测序结果表明获得君子兰酸性转化酶基因家族的一个成员CMCW1,该基因片段长518 bp,不含内含子,编码172个氨基酸。其序列已在GenBank中登记(登记号为AY151269)。在GenBank中进行同源性检索的结果表明,该成员编码的氨基酸与其它植物细胞壁酸性转化酶编码的氨基酸同源性较高。

关键词: 君子兰, 转化酶, 基因, 克隆

Abstract: A pair of primers,designed from conserved regions of plant acid invertase genes,was used to amplify same gene by polymerase chain reaction(PCR).As a result,one 518 bp fragment was obtained,and then cloned into pGEM-TEasy and sequenced.It has no intron and encoded 172 amino acids.This
gene has been registered in GenBank with accession number AY151269.The deduced amino acid sequence is higher identical to the gene from other plant.

Key words: Clivia miniata, Invertase, Gene, Cloning