卷丹侧生器官边界域基因LlLBD18的克隆和功能分析

doi:10.16420/j.issn.0513-353x.2022-0766

园艺学报 ›› 2023, Vol. 50 ›› Issue (10): 2117-2127.doi: 10.16420/j.issn.0513-353x.2022-0766

• 遗传育种·种质资源·分子生物学 • 上一篇下一篇

卷丹侧生器官边界域基因LlLBD18的克隆和功能分析

殷小雨¹^,², 何国仁³, 毕蒙蒙², 唐玉超², 郝春莲¹^,², 渠雨潇², 郝泽慧⁴, 徐雷锋², 胡凤荣¹^,^*(), 杨盼盼²^,^*(), 明军²^,^*()

¹ 南京林业大学风景园林学院，南京 210037
² 中国农业科学院蔬菜花卉研究所，蔬菜生物育种全国重点实验室，北京 100081
³ 上海师范大学生命科学院，上海 200233
⁴ 北京农学院园林学院，北京 102206

收稿日期:2023-05-19 修回日期:2023-10-13 出版日期:2023-10-25 发布日期:2023-10-30
通讯作者: *（E-mail：mingjun@caas.cn，yangpanpan@caas.cn，hufengrong2003@sina.com）
基金资助:
国家自然科学基金项目(31902043); 国家自然科学基金项目(32172612); 国家重点研发计划项目(2019YFD1001002); 中央级公益性科研院所基本科研业务费专项(IVF-BRF2022015)

Cloning and Functional Analysis of LlLBD18 in Lilium lancifolium

YIN Xiaoyu¹^,², HE Guoren³, BI Mengmeng², TANG Yuchao², HAO Chunlian¹^,², QU Yuxiao², HAO Zehui⁴, XU Leifeng², HU Fengrong¹^,^*(), YANG Panpan²^,^*(), MING Jun²^,^*()

¹ College of Landscape Architecture，Nanjing Forestry University，Nanjing 210037，China
² State Key Laboratory of Vegetable Biobreeding，Institute of Vegetables and Flowers，Chinese Academy of Agricultural Sciences，Beijing 100081，China
³ College of Life Sciences，Shanghai Normal University，Shanghai 200233，China
⁴ College of Landscape Architecture，Beijing University of Agriculture，Beijing 102206，China

Received:2023-05-19 Revised:2023-10-13 Published:2023-10-25 Online:2023-10-30
Contact: *（E-mail：mingjun@caas.cn，yangpanpan@caas.cn，hufengrong2003@sina.com）

摘要/Abstract

摘要：

以卷丹（Lilium lancifolium）为试材，克隆了1个LBD转录因子基因，命名为LlLBD18（GenBank序列号ON455210）。其开放阅读框为684 bp，编码227个氨基酸，含有1个典型的LOB结构域。进化树分析发现LlLBD18与姜（Zingiber officinale）ZoLBD18的亲缘关系最近。亚细胞定位结果显示LlLBD18定位于细胞质和细胞核。荧光定量PCR分析表明，LlLBD18在卷丹初生珠芽中表达量最高，其次是根中，在叶片中最低。在珠芽形成过程中，LlLBD18的整体表达情况为先上升后下降再上升，且在珠芽原基启动前期（离体诱导4 d）最高。功能研究发现，在卷丹中过表达LlLBD18可以促进珠芽形成，而沉默LlLBD18后的珠芽形成受到显著抑制，表明LlLBD18在卷丹珠芽形成中起正向调节作用。

关键词: 卷丹, 侧生器官, 珠芽形成, 基因克隆, 功能分析

Abstract:

In this study，a LBD transcription factor gene，named LlLBD18（GenBank accession number ON455210）was cloned from Lilium lancifolium. Its open reading frame was 684 bp，which encoded 227 amino acids，and contained a typical LOB domain. The phylogenetic tree analysis showed that LlLBD18 had the highest homology with Zingiber officinale ZoLBD18. Subcellular localization results showed that LlLBD18 was localized in the cytoplasm and nucleus. Quantitative real-time PCR analysis showed that the expression of LlLBD18 was the highest in primary bulbils，followed by roots，and the lowest in leaves. In the process of bulbil formation，the overall expression of LlLBD18 was first increased，then decreased，and then increased，and the highest expression was at the early stage of bulbil primordium initiation（the 4th day）. Functional studies found that overexpression of LlLBD18 in L. lancifolium can promote bulbil formation，while the formation of bulbils after VIGS silencing of LlLBD18 was significantly inhibited. This research suggested that LlLBD18 plays a positive regulatory role in bulbil formation of L. lancifolium.

Key words: Lilium lancifolium, lateral organ, bulbil formation, gene cloning, function analysis

殷小雨, 何国仁, 毕蒙蒙, 唐玉超, 郝春莲, 渠雨潇, 郝泽慧, 徐雷锋, 胡凤荣, 杨盼盼, 明军. 卷丹侧生器官边界域基因LlLBD18的克隆和功能分析[J]. 园艺学报, 2023, 50(10): 2117-2127.

YIN Xiaoyu, HE Guoren, BI Mengmeng, TANG Yuchao, HAO Chunlian, QU Yuxiao, HAO Zehui, XU Leifeng, HU Fengrong, YANG Panpan, MING Jun. Cloning and Functional Analysis of LlLBD18 in Lilium lancifolium[J]. Acta Horticulturae Sinica, 2023, 50(10): 2117-2127.

图/表 7

表1 本研究中的引物序列

Table 1 Primer sequences in this study

用途 Usage	引物名称 Primer name	引物序列（5′-3′） Primer sequence
全长克隆Full length cloning	LBD18-F	ATGAGCTGCAGTACGAGC
	LBD18-R	TCACCTAGAGACAGGAGGAGTA
实时荧光定量PCR Quantitative real-time PCR	q-LBD18-F	TCCTGTGTATGGATGTGTTGC
	q-LBD18-R	AAGCGGCTGTCGGAATTGAA
亚细胞定位Subcellular localization	2300-LBD18-F	tggagaggacagggtacccgggATGAGCTGCAGTACGAGCAG
	2300-LBD18-R	ccatggtactagtgtcgactctagaCCTAGAGACAGGAGGAGTATCCAG
过表达试验Overexpression experiment	3301-LBD18-F	cggtacccggggatcctctagaATGAGCTGCAGTACGAGCAG
	3301-LBD18-R	acgacggccagtgccaagcttTCACCTAGAGACAGGAGGAG
VIGS沉默试验VIGS silence experiment	TRV2-LBD18-F	gtgagtaaggttaccgaattcCCTAGAGCTACCATCTCCTCC
	TRV2-LBD18-R	tccccatggaggccttctagaTCACCTAGAGACAGGAGGAG

图1 卷丹LlLBD18的PCR扩增及分析

Fig.1 PCR amplification and bioinformatics analysis of LlLBD18 in Lilium lancifolium

图2 卷丹与拟南芥LBD18序列比对红色框中为LOB结构域的主要组成部分，红色三角形为高度保守的氨基酸。

Fig. 2 Sequence alignment of LBD18 from Lilium lancifolium and Arabidopsis thaliana The red box is the main component of LOB domain，the red triangle is highly conserved amino acids.

图3 卷丹LlLBD18与其他15个物种LBD18的系统进化树节点处数字表示亲缘关系的远近。

Fig. 3 Phylogenetic tree of LlLBD18 and LBD18 from other 15 species The numbers at node represent the distance of the kinship.

图4 卷丹LlLBD18亚细胞定位

Fig. 4 The subcellular localization of LlLBD18

图5 LlLBD18在卷丹不同组织（A）和珠芽形成过程中（B）的表达模式不同小写字母表示在P < 0.05水平差异显著。

Fig. 5 The expression analysis of LlLBD18 in different tissues (A) and during bulbil formation (B) in Llium lancifolium Different lowercase letters indicate significant differences at P < 0.05.

图6 卷丹LlLBD18瞬时过表达（A ~ C）和VIGS沉默（D ~ F）植株叶腋表型、珠芽诱导率和基因相对表达量对照为pCAMBIA-3301空载处理，OE为pCAMBIA-3301-LlLBD18处理；TRV为空载处理，TRV::LlLBD18为瞬时沉默处理。不同大写字母表示在P < 0.01水平差异显著。

Fig. 6 Leaf axil phenotype, bulbil induction rate, and relative expression of LlLBD18 transient overexpression（A-C）and VIGS silencing（D-F）plants in Lilium lancifolium Control was treated with pCAMBIA-3301 and OE was treated with pCAMBIA-3301-LlLBD18. TRV was empty vector treatment and TRV::LlLBD18 was transient silencing treatment. Different capital letters indicate significant difference at the level of P < 0.01.

参考文献 39

[1]	Abraham-Juárez M J, Martínez-Hernández A, Leyva-González M A, Herrera-Estrella L, Simpson J. 2010. Class I KNOX genes are associated with organogenesis during bulbil formation in Agave tequilana. Journal of Experimental Botany, 61:4055-4067. doi: 10.1093/jxb/erq215 pmid: 20627900
[2]	Berckmans B, Vassileva V, Schmid S P, Maes S, Parizot B, Naramoto S, Magyar Z, Alvim Kamei C L, Koncz C, Bogre L, Persiau G, de Jaeger G, Friml J, Simon R, Beeckman T, De Veylder L. 2011. Auxin-dependent cell cycle reactivation through transcriptional regulation of Arabidopsis E2Fa by lateral organ boundary proteins. Plant Cell, 23:3671-3683. doi: 10.1105/tpc.111.088377 URL
[3]	China Pharmacopoeia Committee. 2020. Chinese Pharmacopoeia. Beijing: Chemical Industry Press. (in Chinese)
	国家药典委员会. 2020. 中华人民共和国药典. 北京: 中国医药科技出版社.
[4]	Fan M, Xu C, Xu K, Hu Y. 2012. LATERAL ORGAN BOUNDARIES DOMAIN transcription factors direct callus formation in Arabidopsis regeneration. Cell Research, 22:1169-1180. doi: 10.1038/cr.2012.63
[5]	Fang D Z, Chen J, Cheng Y, Li Z M, Guo L T, Wang T H, Deng Q, Xu J P, Gao C S. 2021. First report of Fusarium fujikuroi causing bulb rot on Lilium lancifolium in China. Plant Disease, 105:2254-2254.
[6]	Feng Z, Zhu J, Du X, Cui X. 2012. Effects of three auxin-inducible LBD members on lateral root formation in Arabidopsis thaliana. Planta, 236 (4):1227-1237. doi: 10.1007/s00425-012-1673-3 URL
[7]	Flora of China Editorial Committee. 1980. Flora Reipublicae Popularis Sinicae. Tomus 14. Beijing:Science Press. (in Chinese)
	中国科学院中国植物志编辑委员会. 1980. 中国植物志. 第14卷. 北京: 科学出版社.
[8]	Guo M J, Thomas J, Collins G, Timmermans M C P. 2008. Direct repression of KNOX loci by the ASYMMETRIC LEAVES1 complex of Arabidopsis. Plant Cell, 20:48-58. doi: 10.1105/tpc.107.056127 URL
[9]	He G R, Yang P P, Cao Y W, Tang Y C, Wang L, Song M, Wang J, Xu L F, Ming J. 2021. Cytokinin type-B response regulators promote bulbil initiation in Lilium lancifolium. International Journal of Molecular Sciences, 22 (7):3320. doi: 10.3390/ijms22073320 URL
[10]	He G R, Yang P P, Tang Y C, Cao Y W, Qi X, Xu L F, Ming J. 2020. Mechanism of exogenous cytokinins inducing bulbil formation in Lilium lancifolium in vitro. Plant Cell Reports, 39:861-872. doi: 10.1007/s00299-020-02535-x
[11]	He Guo-ren. 2021. Molecular mechanism of cytokinin promoting bulbil formation in Lilium [Ph. D. Dissertation]. Beijing: Chinese Academy of Agricultural Sciences. (in Chinese)
	何国仁. 2021. 细胞分裂素调控百合珠芽形成的分子机制[博士论文]. 北京: 中国农业科学院.
[12]	Kang N Y, Lee H W, Kim J. 2013. The AP2/EREBP gene PUCHI co-acts with LBD16/ASL18 and LBD18/ASL20 downstream of ARF7 and ARF19 to regulate lateral root development in Arabidopsis. Plant and Cell Physiology, 54 (8):1326-1334. doi: 10.1093/pcp/pct081 URL
[13]	Kim M, Kim M J, Pandey S, Kim J. 2016. Expression and protein interaction analyses reveal combinatorial interactions of LBD transcription factors during Arabidopsis pollen development. Plant Cell Physiology, 57:2291-2299. doi: 10.1093/pcp/pcw145 URL
[14]	Kim M J, Kim M, Lee M R, Park S K, Kim J. 2015. LATERAL ORGAN BOUNDARIES DOMAIN (LBD)10 interacts with SIDECAR POLLEN/LBD27 to control pollen development in Arabidopsis. Plant Journal, 81 (5):794-809. doi: 10.1111/tpj.2015.81.issue-5 URL
[15]	Lee H W, Kang N Y, Pandey S K, Cho C, Lee S H, Kim J. 2017. Dimerization in LBD16 and LBD18 transcription factors is critical for lateral root formation. Plant Physiology, 174:301-311. doi: 10.1104/pp.17.00013 URL
[16]	Lee H W, Kim J. 2013. EXPANSINA17 up-regulated by LBD18/ASL20 promotes lateral root formation during the auxin response. Plant and Cell Physiology, 54:1600-1611. doi: 10.1093/pcp/pct105 URL
[17]	Lee H W, Kim N Y, Lee D J, Kim J. 2009. LBD18/ASL20 regulates lateral root formation in combination with LBD16/ASL18 downstream of ARF7and ARF19 in Arabidopsis. Plant Physiology, 151 (3):1377-1389. doi: 10.1104/pp.109.143685 URL
[18]	Li A, Zhang Y, Wu X, Tang W, Wu R, Dai Z, Liu G, Zhang H, Wu C, Chen G, Pan X. 2008. DH1,a LOB domain-like protein required for glume formation in rice. Plant Molecular Biology, 66:491-502. doi: 10.1007/s11103-007-9283-3 pmid: 18180880
[19]	Li C, Wang J, Li L, Li J, Zhuang M, Li B, Li Q, Huang J, Du Y, Wang J, Fan Z, Mao X, Jing R. 2021. TaMOR is essential for root initiation and improvement of root system architecture in wheat. Plant Biotechnology Journal, 20 (5):862-875. doi: 10.1111/pbi.v20.5 URL
[20]	Li C, Zhu S, Zhang H, Chen L, Cai M, Wang J, Chai J, Wu F, Cheng Z, Guo X, Zhang X, Wan J. 2017. OsLBD37 and OsLBD38,two class II type LBD proteins,are involved in the regulation of heading date by controlling the expression of Ehd1 in rice. Biochemical Biophysical Research Communications, 486:720-725. doi: 10.1016/j.bbrc.2017.03.104 URL
[21]	Liang Yun, Yuan Su-xia, Feng Hui-ying, Xu Lei-feng, Yuan Ying-ying, Liu Chun, Ming Jun. 2013. Cloning and expression analysis of actin gene (lilyActin)from lily. Acta Horticulturae Sinica, 40 (7):1318-1326. (in Chinese)
	梁云, 袁素霞, 冯慧颖, 徐雷锋, 袁迎迎, 刘春, 明军. 2013. 百合肌动蛋白基因 lilyActin 的克隆与表达分析. 园艺学报, 40 (7):1318-1326.
[22]	Lin W C, Shuai B, Springer P S. 2003. The Arabidopsis LATERAL ORGAN BOUNDARIES-domain gene ASYMMETRIC LEAVES2functions in the repression of KNOX gene expression and in adaxial-abaxial patterning. Plant Cell, 15:2241-2252.
[23]	Livak K J, Schmittgen T D. 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2 ^-ΔΔCt method. Methods, 25:402-408. doi: 10.1006/meth.2001.1262 pmid: 11846609
[24]	Ma X J, Tao Y, Zhao X Y, Zhang X S. 2007. Wheat TaAS2,a member of LOB family,affects the adaxial-abaxial polarity of leaves in transgenic Arabidopsis. Plant Science, 172:181-188. doi: 10.1016/j.plantsci.2006.08.008 URL
[25]	Okushima Y, Fukaki H, Onoda M, Theologis A, Tasaka M. 2007. ARF7 and ARF19 regulate lateral root formation via direct activation of LBD/ASL genes in Arabidopsis. Plant Cell, 19:118-130. doi: 10.1105/tpc.106.047761 pmid: 17259263
[26]	Shao Chun-xin. 2006. Studies on establishment of in vitro plant regeneration system from bulblets of Lilium lancifolium[M. D. Dissertation]. Beijing: Beijing Forestry University. (in Chinese)
	邵春昕. 2006. 西藏卷丹珠芽植株再生体系建立研究[硕士论文]. 北京: 北京林业大学.
[27]	Shuai B, Reynaga-Pena C G, Springer P S. 2002. The LATERAL ORGAN BOUNDARIES gene defines a novel,plant-specific gene family. Plant Physiology, 129:747-761.
[28]	Sun X, Feng Z, Meng L, Zhu J, Geitmann A. 2013. Arabidopsis ASL11/LBD15 is involved in shoot apical meristem development and regulates WUS expression. Planta, 237:1367-1378. doi: 10.1007/s00425-013-1844-x URL
[29]	Tang Yu-chao. 2019. The mechanism of auxin involving in bulbil formation of Lilium lancifolium[M. D. Dissertation]. Beijing: Chinese Academy of Agricultural Sciences. (in Chinese)
	唐玉超. 2019. 生长素参与卷丹珠芽形成的研究机制[硕士论文]. 北京: 中国农业科学院.
[30]	Wang C N, Moller M, Cronk Q C. 2004. Altered expression of GFLO,the Gesneriaceae homologue of FLORICAULA/LEAFY,is associated with the transition to bulbil formation in Titanotrichum oldhamii. Development Genes and Evolution, 214:122-127. doi: 10.1007/s00427-004-0388-2 URL
[31]	Wang Shan-shan. 2014. Cloning and identification of chitinase family genes in sugarcane[M. D. Dissertation]. Fuzhou: Fujian Agriculture and Forestry University. (in Chinese)
	王珊珊. 2014. 甘蔗几丁质酶家族基因的克隆与鉴定[硕士论文]. 福州: 福建农林大学.
[32]	Xiang Li, Zhao Lei, Wang Mei, Lü Yi, Wang Yanfang,Shen Xiang,Chen Xuesen,Yin Chengmiao,Mao Zhiquan. 2022. Cloning and functional analysis of apple MdWRKY74. Acta Horticulturae Sinica, 49 (3):482-492. (in Chinese)
	相立, 赵蕾, 王玫, 吕毅, 王艳芳, 沈向, 陈学森, 尹承苗, 毛志泉. 2022. 苹果MdWRKY74的克隆和功能分析. 园艺学报, 49 (3):482-492. doi: 10.16420/j.issn.0513-353x.2021-0244 URL
[33]	Xu L, Xu Y, Dong A W, Sun Y, Pi L M, Xu Y Q, Huang H. 2003. Novel as1 and as2 defects in leaf adaxial-abaxial polarity reveal the requirement for ASYMMETRIC LEAVES1and 2 and ERECTA functions in specifying leaf adaxial identity. Development, 130:4097-4107. doi: 10.1242/dev.00622 URL
[34]	Yang P P, Xu L F, Xu H, He G R, Feng Y Y, Cao Y W, Tang Y C, Yuan S X, Ming J. 2018. Morphological and anatomical observation during the formation of bulbils in Lilium lancifolium. Caryologia, 71:146-149. doi: 10.1080/00087114.2018.1449489 URL
[35]	Yang Pan-pan. 2018. Elucidation of bulbil formation mechanism in Lilium lancifolium and LlAGO1gene cloning[Ph. D. Dissertation]. Nanjing: Nanjing Forestry University. (in Chinese)
	杨盼盼. 2018. 卷丹珠芽形成机理解析及LlAGO1基因克隆[博士论文]. 南京: 南京林业大学.
[36]	Yang Panpan, Xu Hua, Xu Leifeng, Tang Yuchao, He Guoren, Cao Yuwei, Yuan Suxia, Ren Junfang, Ming Jun. 2018. Cloning and expression analysis of LlAGO1 gene in Lilium lancifolium. Acta Horticulturae Sinica, 45 (4):784-794. (in Chinese)
	杨盼盼, 徐华, 徐雷锋, 唐玉超, 何国仁, 曹雨薇, 袁素霞, 任君芳, 明军. 2018. 卷丹LlAGO1基因的克隆及表达分析. 园艺学报, 45 (4):784-794. doi: 10.16420/j.issn.0513-353x.2017-0601
[37]	Ye L L, Wang X, Lyu M, Siligato R, Eswaran G, Vainio L, Blomster T, Zhang J, Mahonen A P. 2021. Cytokinins initiate secondary growth in the Arabidopsis root through a set of LBD genes. Current Biology, 31 (15):3365-3373. doi: 10.1016/j.cub.2021.05.036 URL
[38]	Zhang J, Tang W, Huang Y, Niu X, Zhao Y, Han Y, Liu Y. 2015. Down-regulation of a LBD-like gene,OsIG1,leads to occurrence of unusual double ovules and developmental abnormalities of various floral organs and megagametophyte in rice. Journal of Experimental Botany, 66:99-112. doi: 10.1093/jxb/eru396 URL
[39]	Zhang Y W, Li Z W, Ma B, Hou Q C, Wan X Y. 2020. Phylogeny and functions of LOB domain proteins in plants. International Journal of Molecular Sciences, 21 (7):2278. doi: 10.3390/ijms21072278 URL

卷丹侧生器官边界域基因LlLBD18的克隆和功能分析

Cloning and Functional Analysis of LlLBD18 in Lilium lancifolium

RichHTML

PDF

可视化

摘要/Abstract

引用本文

使用本文

图/表 7

参考文献 39

相关文章 15

编辑推荐

Metrics

本文评价

访问总数:　当日访问总数:　当前在线人数:

[1]	刘慧, 殷向静, 方景浩, 高敏, 李智, 王西平. 中国野生葡萄芪合成酶基因STS19及其启动子的克隆与功能分析[J]. 园艺学报, 2023, 50(7): 1389-1401.
[2]	高鹏飞, 高冰, 冯郑红, 吴建慧. 绢毛委陵菜PsWRKY40的克隆与耐镉功能分析[J]. 园艺学报, 2023, 50(6): 1269-1283.
[3]	吴秀兰, 李桂花, 唐文武. 无籽沙糖橘泛素结合酶E2基因CrUBC2的克隆及功能分析[J]. 园艺学报, 2023, 50(10): 2069-2078.
[4]	王沙, 张心慧, 赵玉洁, 李变变, 招雪晴, 沈雨, 董建梅, 苑兆和. 石榴花青苷合成相关基因PgMYB111的克隆与功能分析[J]. 园艺学报, 2022, 49(9): 1883-1894.
[5]	林元秘, 朱文姣, 陈敏, 薛春梅, 晋芳宇, 朱羽平, 蒋欣玥, 叶凌峰, 倪姝南伶, 杨清. miR396b负调控茄子对黄萎病的防御反应[J]. 园艺学报, 2022, 49(8): 1713-1722.
[6]	邱子文, 刘林敏, 林永盛, 林晓洁, 李永裕, 吴少华, 杨超. 千层金MbEGS基因的克隆与功能分析[J]. 园艺学报, 2022, 49(8): 1747-1760.
[7]	郑林, 王帅, 刘语诺, 杜美霞, 彭爱红, 何永睿, 陈善春, 邹修平. 柑橘响应黄龙病菌侵染的NAC基因的克隆及表达分析[J]. 园艺学报, 2022, 49(7): 1441-1457.
[8]	刘瑶瑶, 吴严严, 石岩, 毛天宇, 包满珠, 张俊卫, 张杰. 垂枝与直枝梅花PmTAC1启动子序列差异与垂枝性状的关系初探[J]. 园艺学报, 2022, 49(6): 1327-1338.
[9]	赵建荣, 杨圆, 秦改花, 刘春燕, 于晴, 贾波涛, 苏颖, 曹榛, 黎积誉. 石榴HAK/KUP/KT家族基因鉴定及钾转运功能分析[J]. 园艺学报, 2022, 49(4): 758-768.
[10]	相立, 赵蕾, 王玫, 吕毅, 王艳芳, 沈向, 陈学森, 尹承苗, 毛志泉. 苹果MdWRKY74的克隆和功能分析[J]. 园艺学报, 2022, 49(3): 482-492.
[11]	宋蒙飞, 查高辉, 陈劲枫, 娄群峰. 黄瓜株型性状分子基础研究进展[J]. 园艺学报, 2022, 49(12): 2683-2702.
[12]	李茂福, 杨媛, 王华, 范又维, 孙佩, 金万梅. 月季自交不亲和性S-RNase的鉴定与分析[J]. 园艺学报, 2022, 49(1): 157-165.
[13]	卞诗村, 陆雅妮, 许吴俊, 陈伯清, 王广龙, 熊爱生. 大蒜生物钟基因AsRVE1和AsRVE2及其在渗透胁迫下的表达分析[J]. 园艺学报, 2021, 48(9): 1706-1716.
[14]	马俊杰, 宋丽娜, 李乐, 马晓春, 靳磊, 徐伟荣. 山葡萄VaCBL6参与非生物胁迫和ABA途径的响应[J]. 园艺学报, 2021, 48(6): 1079-1093.
[15]	苏立遥, 王培育, 蒋梦琦, 黄倏祺, 薛晓东, 刘梦雨, 肖学宸, 赖春旺, 张梓浩, 陈裕坤, 赖钟雄, 林玉玲. 龙眼pri-miR319a编码短肽活性的研究[J]. 园艺学报, 2021, 48(5): 908-920.

卷丹侧生器官边界域基因LlLBD18的克隆和功能分析

Cloning and Functional Analysis of LlLBD18 in Lilium lancifolium

RichHTML

PDF

可视化

摘要/Abstract

引用本文

使用本文

图/表 7

参考文献 39

相关文章 15

编辑推荐

Metrics

本文评价

访问总数: 当日访问总数: 当前在线人数:

访问总数:　当日访问总数:　当前在线人数: