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园艺学报 ›› 2022, Vol. 49 ›› Issue (4): 758-768.doi: 10.16420/j.issn.0513-353x.2021-0202

• 研究论文 • 上一篇    下一篇

石榴HAK/KUP/KT家族基因鉴定及钾转运功能分析

赵建荣1, 杨圆1,2, 秦改花2,*(), 刘春燕2, 于晴2,3, 贾波涛2, 苏颖2,3, 曹榛2, 黎积誉2,*()   

  1. 1.安徽科技学院资源与环境学院 安徽凤阳 233100
    2.安徽省农业科学院园艺研究所,园艺作物种质创制与生理生态安徽省重点实验室,果树果实发育与品质生物学重点实验室,合肥 230001
    3.南京农业大学园艺学院,南京 210095
  • 收稿日期:2021-11-23 修回日期:2022-01-12 出版日期:2022-04-25 发布日期:2022-04-24
  • 通讯作者: 秦改花,黎积誉 E-mail:qghahstu@163.com;lijiyugx@163.com
  • 基金资助:
    安徽省科技重大专项(201903b06020017);安徽省科技重大专项(18030701214);淮北市科技重大专项(HK2021014)

Identification and Functional Analysis of HAK/KUP/KT Family Genes in Pomegranate

ZHAO Jianrong1, YANG Yuan1,2, QIN Gaihua2,*(), LIU Chunyan2, YU Qing2,3, JIA Botao2, SU Ying2,3, CAO Zhen2, LI Jiyu2,*()   

  1. 1. College of Resource and Environment,Anhui Science and Technology University,Fengyang,Anhui 233100,China
    2. Key Laboratory of Horticultural Crop Genetic Improvement and Eco-physiology of Anhui Province,Key Laboratory of Fruit Quality and Developmental Biology,Institute of Horticulture Research,Anhui Academy of Agricultural Sciences,Hefei 230001,China
    3. College of Horticulture,Nanjing Agricultural University,Nanjing 210095,China
  • Received:2021-11-23 Revised:2022-01-12 Online:2022-04-25 Published:2022-04-24
  • Contact: QIN Gaihua,LI Jiyu E-mail:qghahstu@163.com;lijiyugx@163.com

摘要:

从石榴基因组中鉴定出18个编码689 ~ 837个氨基酸的HAK/KUP/KT家族基因;启动子区含有多个与逆境胁迫相关的调控元件,编码蛋白中均含有一段K+转运结构域(GVVYGDLGTSPLY);系统进化分析表明,石榴、拟南芥、葡萄和梨的HAK/KUP/KT家族基因均可分为4个簇(Ⅰ ~ Ⅳ)。RNA-seq分析发现簇Ⅱ和簇Ⅲ的多数基因在石榴不同组织中均有表达,簇Ⅰ中PgrHAK5仅在根中特异性表达。qRT-PCR分析发现,PgrHAK5在石榴根中有特异性高表达,其表达受缺钾胁迫的诱导。亚细胞定位表明PgrHAK5定位于细胞质膜上。酵母功能互补试验表明,PgrHAK5具有转运K+的功能,推测定位于质膜上的PgrHAK5参与石榴根系K+的跨质膜转运。

关键词: 石榴, 钾转运蛋白, PgrHAK5, 功能分析

Abstract:

In order to fully understand the mechanism of K+ uptake,bioinformatics analysis and function study of related genes of HAK/KUP/KT family in pomegranate were carried out in this study. Eighteen of HAK/KUP/KT family genes each encoded 689-837 amino acids were identified from the pomegranate genome. The promoter region contains several regulatory elements related to stress,and the gene-coding proteins contain a K+ transport domain(GVVYGDLGTSPLY). Phylogenetic analysis showed that the HAK/KUP/KT family genes of pomegranate,Arabidopsis thaliana,grape and pear could be divided into four clusters(Ⅰ-Ⅳ). RNA-seq analysis showed that most genes of cluster Ⅱ and cluster Ⅲ were expressed in different tissues of pomegranate,while PgrHAK5 in clusterⅠwas only specifically expressed in roots. qRT-PCR analysis showed that PgrHAK5 was highly expressed in roots,and its expression was induced by potassium deficiency stress. Subcellular localization indicated that the protein encoded by PgrHAK5 was localized on the plasma membrane,and PgrHAK5 can complement the defect of the yeast K+ transport mutant CY162,which suggest that PgrHAK5 play an important role in K+ transport in pomegranate roots.

Key words: pomegranate, potassium transporters, PgrHAK5, functional

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