结球甘蓝染色体片段替换系构建

doi:10.16420/j.issn.0513-353x.2021-0985

园艺学报 ›› 2023, Vol. 50 ›› Issue (1): 65-78.doi: 10.16420/j.issn.0513-353x.2021-0985

结球甘蓝染色体片段替换系构建

崔建¹^,², 钟雄辉², 刘泽慈², 陈登辉², 李海龙², 韩睿², 乐祥庆², 康俊根²^,^*(), 王超¹^,^*()

¹东北农业大学园艺园林学院，哈尔滨 150030
²北京市农林科学院蔬菜研究所，农业农村部华北地区园艺作物生物学与种质创制重点实验室，北京 100097

收稿日期:2022-08-17 修回日期:2022-11-17 出版日期:2023-01-25 发布日期:2023-01-18
通讯作者: *（E-mail：wangchao504@126.com，kangjungen@nercv.org）
基金资助:
国家自然科学基金项目(31972408);北京市农林科学院创新能力建设专项(KJCX20200410);北京市农林科学院创新能力建设专项(KJCX20210425);北京市农林科学院创新能力建设专项(KJCX20200113);北京市农林科学院蔬菜研究所改革与发展项目(KYCX202106)

Construction of Cabbage Chromosome Segment Substitution Lines

CUI Jian¹^,², ZHONG Xionghui², LIU Zeci², CHEN Denghui², LI Hailong², HAN Rui², YUE Xiangqing², KANG Jungen²^,^*(), WANG Chao¹^,^*()

¹School of Horticulture and Landscape Architecture，Northeast Agricultural University，Harbin 150030，China
²Key Laboratory of Biology and Genetic Improvement of Horticultural Crops，Ministry of Agriculture，Beijing Vegetable Research Center，Beijing Academy of Agriculture and Forestry Sciences，Beijing 100097，China

Received:2022-08-17 Revised:2022-11-17 Online:2023-01-25 Published:2023-01-18
Contact: *（E-mail：wangchao504@126.com，kangjungen@nercv.org）

摘要/Abstract

摘要：

以结球甘蓝（Brassica oleracea L. var. capitata）育种骨干亲本R2-P2（圆球形，抽薹晚，硫苷含量低，高感黑腐病、枯萎病和霜霉病）为轮回亲本，以野生甘蓝自交系R4-P1（不结球，抽薹早，蜡质厚，硫苷含量高，对黑腐病、枯萎病和霜霉病等多种病害具有抗性）为供体亲本，通过连续回交、自交和分子标记辅助选择，构建出一套由163个具有R2-P2遗传背景且具有R4-P1供体染色体片段单株组成的染色体片段替换系（Chromosome segment substitution line）。该替换系群体具有102个染色体替换片段，均匀分布在1 ~ 9号染色体上，平均每个株系携带1.3个染色体片段，平均替换长度为5.62 Mb。替换片段总长度为573.74 Mb，覆盖亲本R4-P1全基因组的73.4%，其中单片段替换片段总长度为139.91 Mb，覆盖全基因组的26.5%，双片段替换片段总长231.41 Mb，覆盖亲本R4-P1全基因组的43.82%，含有3个替换片段总长度为201.3 Mb，覆盖供体亲本基因组25.98%。

关键词: 结球甘蓝, 染色体片段替换系, 全基因组, 分子标记, QTL定位

Abstract:

In this experiment，cabbage R2-P2，a foundation parent with head，late bolting and low glucosinolate content，high susceptibility to black rot，Fusarium wilt and downy mildew was served as the recurrent parent. On the contrary，the donor parent R4P1 is a wild cabbage inbred line R4-P1 with no-heading，early bolting，thick wax，high glucosinolate content，and resistance to black rot，Fusarium wilt，downy mildew and other diseases. Resultly，the Chromosome segment substitution lines（CSSLs）was both possessed the genetic background of R2-P2 and individual donor fragment of R4-P1. The CSSLs with 163 lines was constructed by continuous backcross，selfing and marker-assisted selection. The CSSLs contained 102 substituted segments totally，which were derived from genome of donor parent R4-P1 and evenly distributed on nine chromosomes. There were average 1.3 chromosome fragments on a line at least. The whole length of the substituted segments was 573.74 Mb with an average length of 5.62 Mb，which covered the 73.4% of complete DNA sample of R4-P1. Among them，139.91 Mb of single substituted segments covered the 26.5% of complete DNA. Besides，the double-segment replacement fragments were 231.41 Mb，which was accounted for 43.82%. Meanwhile，the total length of three replacement fragments was 201.3 Mb，covering 25.98% of the donor parent genome.

Key words: cabbage, Chromosome segment substitution line, genome, molecular marker, QTL

中图分类号:

S635.1

崔建, 钟雄辉, 刘泽慈, 陈登辉, 李海龙, 韩睿, 乐祥庆, 康俊根, 王超. 结球甘蓝染色体片段替换系构建[J]. 园艺学报, 2023, 50(1): 65-78.

CUI Jian, ZHONG Xionghui, LIU Zeci, CHEN Denghui, LI Hailong, HAN Rui, YUE Xiangqing, KANG Jungen, WANG Chao. Construction of Cabbage Chromosome Segment Substitution Lines[J]. Acta Horticulturae Sinica, 2023, 50(1): 65-78.

图/表 8

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染色体 Chremosome	标记数 Number of marker	平均距离/Mb A average distance between 2 markers	相邻标记最大距离/Mb Maximum distance between adjacent markers
C01	16	1.76	9.69
C02	10	4.22	9.50
C03	7	4.60	16.86
C04	15	2.45	7.66
C05	10	2.34	5.59
C06	7	5.02	10.24
C07	23	1.20	12.58
C08	16	1.16	12.40
C09	13	2.04	18.66
合计Total	113	2.75

染色体 Chremosome	标记数 Number of marker	平均距离/Mb A average distance between 2 markers	相邻标记最大距离/Mb Maximum distance between adjacent markers
C01	16	1.76	9.69
C02	10	4.22	9.50
C03	7	4.60	16.86
C04	15	2.45	7.66
C05	10	2.34	5.59
C06	7	5.02	10.24
C07	23	1.20	12.58
C08	16	1.16	12.40
C09	13	2.04	18.66
合计Total	113	2.75

染色体 Chromosome	代换片段数 Number	长度范围/Mb Lengh range	总长度/Mb Total length	平均长度/Mb Average length	覆盖长度/Mb Coverage length
1	12	0.45 ~ 9.69	41.79	3.49	41.79
2	11	0.24 ~ 18.67	101.11	9.19	38.22
3	4	4.61 ~ 9.13	24.65	6.17	19.79
4	14	0.27 ~ 13.41	59.81	4.27	34.90
5	7	0.80 ~ 5.59	17.91	2.56	17.91
6	8	0.26 ~ 26.66	95.70	11.96	32.14
7	17	0.06 ~ 14.69	54.83	3.22	38.19
8	12	0.13 ~ 12.40	42.79	3.57	38.97
9	17	0.49 ~ 46.30	135.21	7.95	49.23
总计Total	102	0.81 ~ 17.39	573.80	5.82	532.00

染色体 Chromosome	代换片段数 Number	长度范围/Mb Lengh range	总长度/Mb Total length	平均长度/Mb Average length	覆盖长度/Mb Coverage length
1	12	0.45 ~ 9.69	41.79	3.49	41.79
2	11	0.24 ~ 18.67	101.11	9.19	38.22
3	4	4.61 ~ 9.13	24.65	6.17	19.79
4	14	0.27 ~ 13.41	59.81	4.27	34.90
5	7	0.80 ~ 5.59	17.91	2.56	17.91
6	8	0.26 ~ 26.66	95.70	11.96	32.14
7	17	0.06 ~ 14.69	54.83	3.22	38.19
8	12	0.13 ~ 12.40	42.79	3.57	38.97
9	17	0.49 ~ 46.30	135.21	7.95	49.23
总计Total	102	0.81 ~ 17.39	573.80	5.82	532.00

相关性状 Correlated character	基因或QTL Gene or QTL	连锁标记 Linked marker	参考文献 Reference	染色体物理位置 Physical position in chromosome	群体中的位置 Closest markers in the genetic map	替换片段长/Mb Length of replacement segment	连锁单株号 Serial number of linkage line
抗根肿病 Tolerance clubro	Pb(Anju)1^a	KBrH059L13	Nagaoka et al.，2010	C02:24842317 ~ 24842620	8C022 ~ BoSF2745	7.98	29
抗黑腐病 Resistance to black rot	BRQTL-C1-1^a Bo1g056920^b	BbRSdcaps-11	Lee et al.，2015	C01:14884502 ~ 16579946 C01:25091903 ~ 25095843	BoSF1426 ~ BoSF2345 BoSF1245 ~ BoABL1	6.74 9.69	5
	QTL-1^a	BoCL5989s	Kifuji et al.，2013	C02	8C022 ~ BoSF2745	7.98	29
枯萎病 Blight	FOC^a re-Bol037156^b	V17 ~ S9	Lv et al.，2014	C06:40618342 ~ 40705720 C06	8C0464 ~ BoE569	5.21	95
抽薹 Bolting	FLC^b	—	Lin et al.，2005	C03:2116755 ~ 2137112	S31 ~ CG750001	4.86	41
	FLC1^a	PC14 ~ AC-CATR14	Razi et al.，2008	C09	BoSF2645 ~ CK610011	18.66	163
	CO^a	PO118E2~PO160E1	Bohuon et al.，1998	C09	BoSF2645 ~ CK610011	18.66	163
耐裂球 Crack- resistant ball	SPL-2-2^a SPL-4-1^a	BRPGM06 BRMS111	Pang et al.，2015	C02 C04	8C022 ~ BoSF2745 CD834770 ~ BoSF1269	7.98 5.75	— 65

结球甘蓝染色体片段替换系构建

Construction of Cabbage Chromosome Segment Substitution Lines

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结球甘蓝染色体片段替换系构建

Construction of Cabbage Chromosome Segment Substitution Lines

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