不同浓度次氯酸钠处理芹菜种子对其萌发和幼苗质量的影响

doi:10.16420/j.issn.0513-353x.2021-0807

园艺学报 ›› 2022, Vol. 49 ›› Issue (9): 1907-1921.doi: 10.16420/j.issn.0513-353x.2021-0807

不同浓度次氯酸钠处理芹菜种子对其萌发和幼苗质量的影响

谭杉杉¹, 仇亮¹^,³, 段奥其¹, 束胜¹^,², 曾晓萍⁴, 朱为民⁵, 贾敏¹, 阎君⁵, 刘燕花¹, 刘慧¹, 熊爱生¹()

¹南京农业大学园艺学院,作物遗传与种质创新国家重点实验室,南京 210095
²宿迁市设施园艺研究院,江苏宿迁 223800
³江苏沿江地区农业科学研究所,江苏南通 226001
⁴江苏省农业技术推广总站,南京 210036
⁵上海市农业科学院设施园艺研究所,上海 201403

收稿日期:2022-01-04 修回日期:2022-06-10 出版日期:2022-09-25 发布日期:2022-10-08
基金资助:
江苏省种业振兴揭榜挂帅项目(JBGS2021-068);江苏省重点研发计划现代农业项目(BE2022386);宿迁市重点研发计划项目(L202108);国家自然科学基金项目(31272175);江苏高校优势学科建设项目(PAPD)

Effects of Different Concentrations of Sodium Hypochlorite on Seeds Germination and Seedlings Quality of Celery

TAN Shanshan¹, QIU Liang¹^,³, DUAN Aoqi¹, SHU Sheng¹^,², ZENG Xiaoping⁴, ZHU Weimin⁵, JIA Min¹, YAN Jun⁵, LIU Yanhua¹, LIU Hui¹, XIONG Aisheng¹()

¹State Key Laboratory of Crop Genetics and Germplasm Enhancement,College of Horticulture,Nanjing Agricultural University,Nanjing 210095,China
²Suqian Academy of Protected Horticultures,Suqian,Jiangsu 223800,China
³Institute of Agricultural Sciences in Riverside Region of Jiangsu,Nantong,Jiangsu 226001,China
⁴Jiangsu Agricultural Technical Extension Station,Nanjing 210036,China
⁵Horticulture Research Institute,Shanghai Academy Agricultural Sciences,Shanghai 201403,China

Received:2022-01-04 Revised:2022-06-10 Online:2022-09-25 Published:2022-10-08

摘要/Abstract

摘要：

芹菜种子小,且含有大量的萌发抑制物质,给其种苗繁育带来困难。采用培养皿培养和穴盘培养两种方式,用不同浓度（0、5%、10%、20%、40%）NaClO处理‘六合黄心芹’和‘赛雪’芹菜种子,对种子萌发指标、幼苗生长指标及光合参数进行测定,并分析幼苗中叶绿素含量变化以及相关基因表达。结果表明：5%次氯酸钠溶液处理芹菜种子,其发芽率、发芽势、发芽指数和活力指数最高,幼苗生长势最强,幼苗的壮苗指标、叶绿素含量以及净光合速率也有一定程度提高,相关基因的相对表达量也较高。这些结果说明次氯酸钠处理促进芹菜种子的适宜浓度为5%。

关键词: 芹菜, 种子, 次氯酸钠, 萌发, 幼苗, 叶绿素

Abstract:

Celery seeds are small and contain a large amount of germination-inhibiting substances,which brings difficulties to celery seeding propagation. The seeds of‘Liuhe Huangxinqin’and‘Saixue’were treated with different concentrations（0,5%,10%,20%,40%）of NaClO cultured in petri dish and acupoint dish. The seed germination index,seedling growth index and photosynthetic parameters were measured. In addition,the changes of chlorophyll content and related gene expression in seedlings were analyzed. The results showed that celery seeds treated with 5% NaClO had the highest germination rate,germination potential,germination index and vigor index,and the strongest seedling growth potential. And the seedling vigor index,chlorophyll content and net photosynthetic rate were also increased to some extent. Also,the relative expression levels of related genes were also higher. These results suggested that the appropriate concentration for NaClO to promote celery seeds is 5%.

Key words: celery, seed, sodium hypochlorite, germination, seedling, chlorophyll

中图分类号:

S636.3

谭杉杉, 仇亮, 段奥其, 束胜, 曾晓萍, 朱为民, 贾敏, 阎君, 刘燕花, 刘慧, 熊爱生. 不同浓度次氯酸钠处理芹菜种子对其萌发和幼苗质量的影响[J]. 园艺学报, 2022, 49(9): 1907-1921.

TAN Shanshan, QIU Liang, DUAN Aoqi, SHU Sheng, ZENG Xiaoping, ZHU Weimin, JIA Min, YAN Jun, LIU Yanhua, LIU Hui, XIONG Aisheng. Effects of Different Concentrations of Sodium Hypochlorite on Seeds Germination and Seedlings Quality of Celery[J]. Acta Horticulturae Sinica, 2022, 49(9): 1907-1921.

图/表 11

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基因Gene	序列（5′-3′）Sequence
脱植基叶绿素a氧化酶基因 CAO	F：TTGGTGAATGATAGGCTGTT;R：GGATGGTAAGGTTGGACTG
叶绿素b还原酶基因 NOL	F：TCTCTTCTTCGCATCATCTT;R：TTGAGCCAGCATTGTTGA
7-羟甲基叶绿素a还原酶基因 HCAR	F：CCAACTTAGCCTTATCATCTG;R：TGCCACAACATCCACAAT
H亚基镁螯合酶基因 ChlH	F：GAATAGTAGCCGCATAGGAT;R：ACAAGAAGGTAGCCATCAC
镁原卟啉氧甲基转移酶基因 ChlM	F：TATCAGTCTCCGCCATTTTCCCTCA;R：GTGTCCCCGTATATTTTCTTCCACC
镁原卟啉Ⅸ单酯环化酶基因 MPE	F：ATGATACACTGAAGCGGATT;R：CCACTAATAAGCAGCAACAA
脱镁叶绿素酶基因 PPH	F：CAAGCAGAGGCATCACAA;R：CCGTATAATGGAGACAACAAG
脱镁叶绿酸氧化酶基因 PAO	F：ATGTGGAGGAACGGAAGA;R：GTAGGCATCTAACGGAAGG
芹菜内参基因 Agactin	F：CTTCCTGCCATATATGATTGG;R：GCCAGCACCTCGATCTTCATG

基因Gene	序列（5′-3′）Sequence
脱植基叶绿素a氧化酶基因 CAO	F：TTGGTGAATGATAGGCTGTT;R：GGATGGTAAGGTTGGACTG
叶绿素b还原酶基因 NOL	F：TCTCTTCTTCGCATCATCTT;R：TTGAGCCAGCATTGTTGA
7-羟甲基叶绿素a还原酶基因 HCAR	F：CCAACTTAGCCTTATCATCTG;R：TGCCACAACATCCACAAT
H亚基镁螯合酶基因 ChlH	F：GAATAGTAGCCGCATAGGAT;R：ACAAGAAGGTAGCCATCAC
镁原卟啉氧甲基转移酶基因 ChlM	F：TATCAGTCTCCGCCATTTTCCCTCA;R：GTGTCCCCGTATATTTTCTTCCACC
镁原卟啉Ⅸ单酯环化酶基因 MPE	F：ATGATACACTGAAGCGGATT;R：CCACTAATAAGCAGCAACAA
脱镁叶绿素酶基因 PPH	F：CAAGCAGAGGCATCACAA;R：CCGTATAATGGAGACAACAAG
脱镁叶绿酸氧化酶基因 PAO	F：ATGTGGAGGAACGGAAGA;R：GTAGGCATCTAACGGAAGG
芹菜内参基因 Agactin	F：CTTCCTGCCATATATGATTGG;R：GCCAGCACCTCGATCTTCATG

品种 Cultivar	NaClO/%	发芽势/% Germination potential		发芽率/% Germination rate		发芽指数 Germination index		活力指数 Vigor index
品种 Cultivar	NaClO/%	培养皿培养 In petri dishes	穴盘培养 In acupoint dishes	培养皿培养 In petri dishes	穴盘培养 In acupoint dishes	培养皿培养 In petri dishes	穴盘培养 In acupoint dishes	培养皿培养 In petri dishes	穴盘培养 In acupoint dishes
六合黄心芹 Liuhe Huangxinqin	0	65.33 a	56.00 ab	82.67 a	70.44 ab	6.82 a	10.08 ab	144.90 a	286.10 b
	5	77.33 a	69.33 a	83.33 a	81.78 a	7.06 a	11.89 a	173.51 a	371.48 a
	10	62.00 a	47.11 bc	76.00 ab	64.89 abc	6.38 ab	9.13 bc	149.86 a	224.99 bc
	20	62.00 a	49.11 b	73.33 ab	62.00 bc	6.09 ab	8.88 bc	156.52 a	211.95 c
	40	42.67 b	31.11 c	62.00 b	51.56 c	4.92 b	6.78 c	79.69 b	110.52 d
赛雪 Saixue	0	59.67 b	61.33 a	76.67 b	73.11 ab	6.14 b	10.60 ab	178.35 b	302.60 b
	5	75.87 a	64.03 a	91.33 a	78.33 a	7.54 a	11.26 a	235.33 a	396.21 a
	10	58.00 b	50.22 b	76.00 bc	68.44 ab	6.11 bc	9.68 bc	159.70 b	310.39 b
	20	61.33 b	49.33 b	72.67 bc	67.33 b	5.95 bc	9.56 bc	182.52 b	209.57 c
	40	47.00 c	41.22 c	62.00 c	64.67 b	4.96 c	8.95 c	79.97 c	223.81 c

品种 Cultivar	NaClO/%	发芽势/% Germination potential		发芽率/% Germination rate		发芽指数 Germination index		活力指数 Vigor index
品种 Cultivar	NaClO/%	培养皿培养 In petri dishes	穴盘培养 In acupoint dishes	培养皿培养 In petri dishes	穴盘培养 In acupoint dishes	培养皿培养 In petri dishes	穴盘培养 In acupoint dishes	培养皿培养 In petri dishes	穴盘培养 In acupoint dishes
六合黄心芹 Liuhe Huangxinqin	0	65.33 a	56.00 ab	82.67 a	70.44 ab	6.82 a	10.08 ab	144.90 a	286.10 b
	5	77.33 a	69.33 a	83.33 a	81.78 a	7.06 a	11.89 a	173.51 a	371.48 a
	10	62.00 a	47.11 bc	76.00 ab	64.89 abc	6.38 ab	9.13 bc	149.86 a	224.99 bc
	20	62.00 a	49.11 b	73.33 ab	62.00 bc	6.09 ab	8.88 bc	156.52 a	211.95 c
	40	42.67 b	31.11 c	62.00 b	51.56 c	4.92 b	6.78 c	79.69 b	110.52 d
赛雪 Saixue	0	59.67 b	61.33 a	76.67 b	73.11 ab	6.14 b	10.60 ab	178.35 b	302.60 b
	5	75.87 a	64.03 a	91.33 a	78.33 a	7.54 a	11.26 a	235.33 a	396.21 a
	10	58.00 b	50.22 b	76.00 bc	68.44 ab	6.11 bc	9.68 bc	159.70 b	310.39 b
	20	61.33 b	49.33 b	72.67 bc	67.33 b	5.95 bc	9.56 bc	182.52 b	209.57 c
	40	47.00 c	41.22 c	62.00 c	64.67 b	4.96 c	8.95 c	79.97 c	223.81 c

品种 Cultivar	NaClO/%	根长/mm Root length		下胚轴长/mm Hypocotyl length		单株鲜质量/mg Fresh weight per plant		单株干质量/mg Dry weight per plant
品种 Cultivar	NaClO/%	培养皿培养 In petri dishes	穴盘培养 In acupoint dishes	培养皿培养 In petri dishes	穴盘培养 In acupoint dishes	培养皿培养 In petri dishes	穴盘培养 In acupoint dishes	培养皿培养 In petri dishes	穴盘培养 In acupoint dishes
六合黄心芹 Liuhe Huangxinqin	0	21.25 ab	28.39 ab	9.59 bc	13.38 b	3.82 d	4.65 c	0.28 c	0.35 ab
	5	24.57 a	31.25 a	10.26 b	13.41 b	5.29 b	5.19 b	0.33 a	0.36 a
	10	23.50 a	24.64 b	14.59 a	10.61 b	5.43 a	5.27 a	0.33 a	0.36 a
	20	25.71 a	23.87 b	8.11 c	13.59 b	5.43 a	5.20 b	0.30 b	0.35 ab
	40	16.21 b	16.31 c	8.17 c	26.91 a	4.14 c	3.32 d	0.29 bc	0.22 c
赛雪Saixue	0	29.03 a	28.56 abc	14.47 a	10.35 b	4.20 d	5.99 c	0.30 c	0.46 b
	5	31.20 a	35.19 a	15.13 a	14.43 b	4.72 b	6.57 b	0.36 b	0.53 a
	10	26.15 ab	32.05 ab	11.51 b	11.07 b	4.80 a	6.99 a	0.47 a	0.42 bc
	20	30.69 a	21.93 c	11.41 b	13.89 b	4.62 c	5.65 d	0.36 b	0.37 d
	40	16.11 b	25.02 bc	8.73 c	20.01 a	4.61 c	6.53 b	0.30 c	0.38 cd

不同浓度次氯酸钠处理芹菜种子对其萌发和幼苗质量的影响

Effects of Different Concentrations of Sodium Hypochlorite on Seeds Germination and Seedlings Quality of Celery

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苗龄/d Seeding age	品种 Cultivar	NaClO/%	株高/cm Height	茎粗/mm Diameter	鲜质量/mg Fresh weight		干质量/mg Dry weight		壮苗指数 Seedling strength index
苗龄/d Seeding age	品种 Cultivar	NaClO/%	株高/cm Height	茎粗/mm Diameter	地上部 Aboveground	地下部 Underground	地上部 Aboveground	地下部 Underground	壮苗指数 Seedling strength index
40	六合黄心芹 Liuhe Huangxinqin	0	12.10 a	0.78 bc	240 b	9.50 a	17.30 c	0.70 b	0.84 c
		5	12.77 a	0.98 a	300 a	9.10 b	21.50 a	0.87 a	1.08 a
		10	10.20 b	0.89 ab	290 a	7.40 c	19.10 b	0.73 b	0.93 b
		20	8.80 b	0.88 ab	180 c	4.10 e	12.10 d	0.47 c	0.61 d
		40	6.93 c	0.72 c	130 d	5.00 d	8.00 e	0.23 d	0.32 e
	赛雪Saixue	0	10.27 b	0.96 a	200 a	9.90 a	11.35 c	0.48 b	0.61 b
		5	12.20 a	0.98 a	210 a	9.90 a	12.50 a	0.83 a	0.99 a
		10	10.43 b	0.75 b	160 c	4.40 b	11.85 b	0.27 d	0.36 d
		20	10.37 b	0.74 b	150 c	4.98 b	9.80 d	0.33 c	0.41 c
		40	10.23 b	0.75 b	180 b	2.78 c	9.85 d	0.30 cd	0.38 cd
60	六合黄心芹 Liuhe Huangxinqin	0	13.20 b	1.03 c	540 b	32.10 b	36.20 b	3.30 bc	3.91 c
		5	16.80 a	1.68 a	580 a	89.60 a	40.00 a	7.70 a	9.66 a
		10	13.40 b	1.22 b	370 c	11.00 c	23.10 c	2.80 c	3.37 d
		20	11.20 c	0.95 cd	240 d	33.40 b	13.90 d	3.50 b	4.53 b
		40	10.40 cd	0.77 d	180 e	5.60 d	8.60 e	0.50 d	0.60 e
	赛雪Saixue	0	12.70 bc	1.02 bc	310 b	24.80 a	19.50 b	2.20 a	2.62 ab
		5	14.70 a	1.40 a	460 a	25.70 a	26.80 a	2.20 a	2.66 a
		10	11.90 d	1.09 b	290 c	22.40 b	17.30 b	1.60 c	1.92 d
		20	12.80 bc	0.95 c	210 d	21.10 b	12.90 c	2.10 ab	2.55 bc
		40	13.40 b	0.86 d	220 d	17.50 c	10.00 cd	1.70 b	2.06 cd

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不同浓度次氯酸钠处理芹菜种子对其萌发和幼苗质量的影响

Effects of Different Concentrations of Sodium Hypochlorite on Seeds Germination and Seedlings Quality of Celery

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参考文献 46

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