https://www.ahs.ac.cn/images/0513-353X/images/top-banner1.jpg|#|苹果
https://www.ahs.ac.cn/images/0513-353X/images/top-banner2.jpg|#|甘蓝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner3.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner4.jpg|#|灵芝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner5.jpg|#|桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner6.jpg|#|黄瓜
https://www.ahs.ac.cn/images/0513-353X/images/top-banner7.jpg|#|蝴蝶兰
https://www.ahs.ac.cn/images/0513-353X/images/top-banner8.jpg|#|樱桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner9.jpg|#|观赏荷花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner10.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner11.jpg|#|月季
https://www.ahs.ac.cn/images/0513-353X/images/top-banner12.jpg|#|菊花

园艺学报 ›› 2022, Vol. 49 ›› Issue (11): 2431-2438.doi: 10.16420/j.issn.0513-353x.2021-0886

• 研究报告 • 上一篇    下一篇

甘肃省苹果坏死花叶病毒检测及遗传多样性分析

李世帆, 邵宇纯, 祁豪男, 杨兰, 李小伟, 徐秉良, 陈雅寒()   

  1. 甘肃农业大学植物保护学院,兰州 730070
  • 收稿日期:2022-06-06 修回日期:2022-08-04 出版日期:2022-11-25 发布日期:2022-11-25
  • 通讯作者: 陈雅寒 E-mail:yhchen1018@nwafu.edu.cn
  • 基金资助:
    甘肃农业大学人才启动金项目(GAU-KYQD-2019-22);甘肃省高等学校创新基金项目(2021B-117);甘肃省自然科学基金项目(21JR7RA820)

Detection and Genetic Diversity Analysis of Apple Necrotic Mosaic Virus in Gansu Province

LI Shifan, SHAO Yuchun, QI Haonan, YANG Lan, LI Xiaowei, XU Bingliang, CHEN Yahan()   

  1. College of Plant Protection,Gansu Agricultural University,Lanzhou 730070,China
  • Received:2022-06-06 Revised:2022-08-04 Online:2022-11-25 Published:2022-11-25
  • Contact: CHEN Yahan E-mail:yhchen1018@nwafu.edu.cn

摘要:

为明确甘肃省苹果坏死花叶病毒(apple necrotic mosaic virus,ApNMV)的发生及遗传变异情况,采用反转录PCR方法对采自甘肃7个地区表现花叶症状和无明显症状的共93份苹果叶片样品进行了检测。结果显示ApNMV的平均检出率为66.67%,说明其在甘肃省苹果产区普遍发生。对其中9份样品中ApNMV的外壳蛋白(coat protein,CP)基因进行了克隆和测序,其分离物CP的核苷酸和编码蛋白氨基酸序列相似性分别为92.3% ~ 99.7%和93.6% ~ 99.1%,与NCBI数据库中来自中国、印度、日本等国家的18个ApNMV分离物核苷酸和氨基酸序列相似性分别为86.7% ~ 99.7%和87.1% ~ 99.7%。基于CP基因全长序列构建的系统发育树,ApNMV分离物聚集成5个组,9个ApNMV甘肃分离物与其他ApNMV分离物分别聚集形成组Ⅰ、组Ⅱ和组Ⅴ。9条ApNMV全长CP基因序列中检测到1个潜在重组事件。

关键词: 苹果, ApNMV, CP, RT-PCR, 遗传多样性, 重组

Abstract:

In order to clarify the incidence and genetic variation of apple necrotic mosaic virus (ApNMV)in Gansu Province,a total of 93 apple leaf samples with mosaic or without obvious symptoms were tested by reverse transcription-polymerase chain reaction(RT-PCR). The results showed that the average incidence of ApNMV among the collected samples was 66.67%,which indicated that this virus spread widely around the surveyed regions of Gansu Province. The full-length coat protein(CP)genes generated from mine samples were cloned and sequenced. The similarity of nucleotide and amino acid sequence identities of ApNMV CP genes of the nine isolates ranged from 92.3%-99.7%,and 93.6%-99.1%,respectively,while they shared that of the available 18 ApNMV isolates from NCBI database of China,India and Japan,which ranged from 86.7%-99.7%,and 87.1%-99.7%,respectively. The phylogenetic tree were constructed based on the complete CP gene,and the ApNMV isolates clustered into five groups,among which,nine ApNMV isolates from Gansu and other ApNMV isolates to form GroupⅠ,GroupⅡand GroupⅤ,respectively. Finally,a potential recombination event was tested among the nine complete nucleotide sequences of ApNMV CP genes.

Key words: apple, ApNMV, CP, RT-PCR genetic diversity, recombination

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