马铃薯资源糖苷生物碱含量评价及合成相关基因表达分析

doi:10.16420/j.issn.0513-353x.2021-0811

园艺学报 ›› 2022, Vol. 49 ›› Issue (11): 2357-2366.doi: 10.16420/j.issn.0513-353x.2021-0811

马铃薯资源糖苷生物碱含量评价及合成相关基因表达分析

叶广继¹^,², 郑贞贞¹, 纳添仓¹^,², 王舰¹^,²^,^**()

¹青海省农林科学院，西宁 810016
²青海省马铃薯育种重点实验室，青藏高原种质资源研究与利用实验室，青海大学青藏高原生物技术教育部重点实验室，省部共建三江源生态与高原农牧业国家重点实验室，西宁 810016

收稿日期:2022-04-07 修回日期:2022-08-17 出版日期:2022-11-25 发布日期:2022-11-25
通讯作者: 王舰 E-mail:jianwang2197@163.com
基金资助:
三江源生态与高原农牧业国家重点实验室开放课题(2014-08);青海省自然科学基础研究项目(2022-ZJ-902);青海省创新平台建设项目(2022-ZJ-Y01)

Evaluation of Steroidal Glycoalkaloids and Analysis of Related Gene Expression in Potato Resources

YE Guangji¹^,², ZHENG Zhenzhen¹, NA Tiancang¹^,², WANG Jian¹^,²^,^**()

¹Qinghai Academy of Agriculture and Forestry Sciences，Xining 810016，China
²Qinghai Provincial Key Laboratory of Potato Breeding，Laboratory for Research and Utilization of Qinghai Tibet Plateau Germplasm Resources，Qinghai University Key Laboratory of Qinghai-Tibet Plateau Biotechnology of the Ministry of Education，Provincial and Ministry Co-construction of the State Key Laboratory of Sanjiangyuan Ecology and Plateau Agriculture and Animal Husbandry，Xining 810016，China

Received:2022-04-07 Revised:2022-08-17 Online:2022-11-25 Published:2022-11-25
Contact: WANG Jian E-mail:jianwang2197@163.com

摘要/Abstract

摘要：

检测11个马铃薯资源叶片和薯皮中糖苷生物碱（Steroidal glycoalkaloids，SGA）含量，筛选低SGA含量的马铃薯资源，并对下寨65、LZ111、Q072625、陇薯7号、青薯9号和青薯11号薯皮中SGA合成途径中相关基因的表达进行分析，以期明确马铃薯SGA生物合成关键基因，为创制低SGA马铃薯资源提供新思路。结果表明：（1）11个马铃薯资源叶片中SGA含量范围为0.08600 ~ 1.58066 mg · g^-1 FW;（2）11个马铃薯资源薯皮中SGA含量范围为0.01415 ~ 0.18450 mg · g^-1 FW，薯皮总SGA含量均小于0.20 mg · g^-1 FW（安全范围）;（3）6个马铃薯资源块茎中SGA合成途径相关基因表达量分析表明，StSQS1、StCAS、StSSR2和StGAME4是马铃薯块茎SGA生物合成的关键基因。

关键词: 马铃薯, 糖苷生物碱, 基因表达

Abstract:

The content of SGAs（Steroidal glycoalkaloids）was evaluated in the leaf and peel tissue of 11 potato resources，the resources with relatively low level of SGAs were selected. The gene expression related to the SGAs biosynthesis was determined in Xiazhai 65，LZ111，Q072625，Longshu 7，Qingshu 9，and Qingshu 11，which would help confirm the key genes in SGAs biosynthesis of potato and provide the potential to create the low SGAs potato resources in the future. These results showed that SGAs content in the leaves of 11 cultivars was 0.08600-1.58066 mg · g^-1 FW;the SGAs content in the peel tissue of potato tubers was 0.01415-0.18450 mg · g^-1 FW and lower than 0.20 mg · g^-1 FW（the safety level）. The genes of StSQS1，StCAS，StSSR2 and StGAME4 played key roles in SGAs biosynthesis.

Key words: Solanum tuberosum, steroidal glycoalkaloids, expression of gene

中图分类号:

S532

叶广继, 郑贞贞, 纳添仓, 王舰. 马铃薯资源糖苷生物碱含量评价及合成相关基因表达分析[J]. 园艺学报, 2022, 49(11): 2357-2366.

YE Guangji, ZHENG Zhenzhen, NA Tiancang, WANG Jian. Evaluation of Steroidal Glycoalkaloids and Analysis of Related Gene Expression in Potato Resources[J]. Acta Horticulturae Sinica, 2022, 49(11): 2357-2366.

图/表 5

参考文献 36

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序号 Number	资源 Resource	块茎成熟期 Tuber mature period	薯皮色 Tuber skin colour	薯肉色 Tuber flesh colour
1	大西洋Daxiyang	早熟 Early maturity	白 White	白 White
2	青薯2号Qingshu 2	晚熟 Late maturity	白 White	白 White
3	青薯9号Qingshu 9	晚熟 Late maturity	红 Red	彩色 Colour
4	青薯10号Qingshu 10	晚熟 Late maturity	白 White	白 White
5	青薯11号Qingshu 11	晚熟 Late maturity	粉 Pink	彩色 Colour
6	陇薯6号Longshu 6	晚熟 Late maturity	白 White	白 White
7	陇薯7号Longshu 7	晚熟 Late maturity	黄 Yellow	黄 Yellow
8	下寨65 Xiazhai 65	中晚熟 Mid-late maturity	黄 Yellow	浅黄 Pale yellow
9	LZ111	晚熟 Late maturity	白 White	白 White
10	D0602-10	中晚熟 Mid-late maturity	白 White	白 White
11	Q072625	晚熟 Late maturity	白 White	白 White

序号 Number	资源 Resource	块茎成熟期 Tuber mature period	薯皮色 Tuber skin colour	薯肉色 Tuber flesh colour
1	大西洋Daxiyang	早熟 Early maturity	白 White	白 White
2	青薯2号Qingshu 2	晚熟 Late maturity	白 White	白 White
3	青薯9号Qingshu 9	晚熟 Late maturity	红 Red	彩色 Colour
4	青薯10号Qingshu 10	晚熟 Late maturity	白 White	白 White
5	青薯11号Qingshu 11	晚熟 Late maturity	粉 Pink	彩色 Colour
6	陇薯6号Longshu 6	晚熟 Late maturity	白 White	白 White
7	陇薯7号Longshu 7	晚熟 Late maturity	黄 Yellow	黄 Yellow
8	下寨65 Xiazhai 65	中晚熟 Mid-late maturity	黄 Yellow	浅黄 Pale yellow
9	LZ111	晚熟 Late maturity	白 White	白 White
10	D0602-10	中晚熟 Mid-late maturity	白 White	白 White
11	Q072625	晚熟 Late maturity	白 White	白 White

序号 Number	资源 Resource	α-茄碱/（mg · g^-1FW） α-Solanine	α-卡茄碱/（mg · g^-1FW） α-Chaconine	总SGA/（mg · g^-1FW） Total SGAs	α-卡茄碱/α-茄碱 α-Chaconine/α-Solanine
1	大西洋Daxiyang	0.01912 ± 0.00061 e	0.02213 ± 0.00075 f	0.04125 ± 0.00136 ef	1.16
2	青薯2号Qingshu 2	0.02625 ± 0.00089 d	0.04165 ± 0.00145 d	0.06790 ± 0.00234 d	1.59
3	青薯9号Qingshu 9	0.00653 ± 0.00007 i	0.00762 ± 0.00020 i	0.01415 ± 0.00027 i	1.17
4	青薯10号Qingshu 10	0.01608 ± 0.00007 f	0.02181 ± 0.00002 f	0.03789 ± 0.00005 f	1.36
5	青薯11号Qingshu 11	0.01233 ± 0.00005 g	0.01952 ± 0.00019 g	0.03185 ± 0.00015 g	1.58
6	陇薯6号Longshu 6	0.01814 ± 0.00024 e	0.02566 ± 0.00084 e	0.04380 ± 0.00107 e	1.41
7	陇薯7号Longshu 7	0.00975 ± 0.00018 h	0.01539 ± 0.00001 h	0.02514 ± 0.00018 h	1.58
8	下寨65 Xiazhai 65	0.06028 ± 0.00161 a	0.07129 ± 0.00209 b	0.13156 ± 0.00370 b	1.18
9	LZ111	0.05233 ± 0.00113 c	0.04757 ± 0.00145 c	0.09990 ± 0.00258 c	0.91
10	D0602-10	0.05528 ± 0.00145 b	0.12922 ± 0.00331 a	0.18450 ± 0.00475 a	2.34
11	Q072625	0.01294 ± 0.00023 g	0.01960 ± 0.00001 g	0.03254 ± 0.00022 g	1.51

序号 Number	资源 Resource	α-茄碱/（mg · g^-1FW） α-Solanine	α-卡茄碱/（mg · g^-1FW） α-Chaconine	总SGA/（mg · g^-1FW） Total SGAs	α-卡茄碱/α-茄碱 α-Chaconine/α-Solanine
1	大西洋Daxiyang	0.01912 ± 0.00061 e	0.02213 ± 0.00075 f	0.04125 ± 0.00136 ef	1.16
2	青薯2号Qingshu 2	0.02625 ± 0.00089 d	0.04165 ± 0.00145 d	0.06790 ± 0.00234 d	1.59
3	青薯9号Qingshu 9	0.00653 ± 0.00007 i	0.00762 ± 0.00020 i	0.01415 ± 0.00027 i	1.17
4	青薯10号Qingshu 10	0.01608 ± 0.00007 f	0.02181 ± 0.00002 f	0.03789 ± 0.00005 f	1.36
5	青薯11号Qingshu 11	0.01233 ± 0.00005 g	0.01952 ± 0.00019 g	0.03185 ± 0.00015 g	1.58
6	陇薯6号Longshu 6	0.01814 ± 0.00024 e	0.02566 ± 0.00084 e	0.04380 ± 0.00107 e	1.41
7	陇薯7号Longshu 7	0.00975 ± 0.00018 h	0.01539 ± 0.00001 h	0.02514 ± 0.00018 h	1.58
8	下寨65 Xiazhai 65	0.06028 ± 0.00161 a	0.07129 ± 0.00209 b	0.13156 ± 0.00370 b	1.18
9	LZ111	0.05233 ± 0.00113 c	0.04757 ± 0.00145 c	0.09990 ± 0.00258 c	0.91
10	D0602-10	0.05528 ± 0.00145 b	0.12922 ± 0.00331 a	0.18450 ± 0.00475 a	2.34
11	Q072625	0.01294 ± 0.00023 g	0.01960 ± 0.00001 g	0.03254 ± 0.00022 g	1.51

序号 Number	资源 Resource	α-茄碱/（mg · g^-1FW） α-Solanine	α-卡茄碱/（mg · g^-1FW） α-Chaconine	总SGA/（mg · g^-1FW） Total SGAs	α-卡茄碱/α-茄碱 α-Chaconine/α-Solanine
1	大西洋Daxiyang	0.56977 ± 0.00268 a	0.71562 ± 0.00140 d	1.28539 ± 0.00408 c	1.26
2	青薯2号Qingshu 2	0.40000 ± 0.00227 e	0.97333 ± 0.00538 b	1.37333 ± 0.00320 b	2.43
3	青薯9号Qingshu 9	0.01667 ± 0.00026 k	0.06933 ± 0.00097 i	0.08600 ± 0.00124 k	4.16
4	青薯10号Qingshu 10	0.43467 ± 0.01179 c	0.76667 ± 0.00586 c	1.20134 ± 0.01747 d	1.76
5	青薯11号Qingshu 11	0.18133 ± 0.00759 h	0.22867 ± 0.00993 h	0.41000 ± 0.01752 i	1.26
6	陇薯6号Longshu 6	0.41533 ± 0.00401 d	0.72667± 0.01983 d	1.14200 ± 0.02383 e	1.75
7	陇薯7号Longshu 7	0.50733 ± 0.01111 b	1.07333 ± 0.04750 a	1.58066 ± 0.05861 a	2.12
8	下寨65 Xiazhai 65	0.13733 ± 0.00087 i	0.37667 ± 0.00741 g	0.51400 ± 0.00828 h	2.74
9	LZ111	0.35533 ± 0.00090 f	0.64133 ± 0.02547 e	0.99666 ± 0.02637 f	1.80
10	D0602-10	0.23867 ± 0.00087 g	0.44933 ± 0.00639 f	0.68800 ± 0.00726 g	1.88
11	Q072625	0.06307 ± 0.00110 j	0.20467 ± 0.00479 h	0.26774 ± 0.00369 j	3.25

马铃薯资源糖苷生物碱含量评价及合成相关基因表达分析

Evaluation of Steroidal Glycoalkaloids and Analysis of Related Gene Expression in Potato Resources

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马铃薯资源糖苷生物碱含量评价及合成相关基因表达分析

Evaluation of Steroidal Glycoalkaloids and Analysis of Related Gene Expression in Potato Resources

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