基于转录组测序的茄子萼下果色光敏相关基因表达分析

doi:10.16420/j.issn.0513-353x.2021-0665

摘要/Abstract

摘要：

为了探究茄子光敏品种和非光敏品种控制萼下果色的差异基因，以萼下紫色的圆茄自交系Y₅和萼下绿色的圆茄自交系Y₇₃萼片下果皮为试材，进行去萼片见光0、4和8 h的转录组测序。共获得75. 61 Gb有效数据（Clean data），Q30碱基百分比均达到93. 97%以上;两个自交系材料在3个时间点的转录组测序比较共获得11 812个差异表达基因，包括5 277个上调基因和6 535个下调基因。3个时间点数据KEGG富集分析同时指向苯丙烷生物合成、类黄酮生物合成和光合生物的固碳作用通路。苯丙烷和类黄酮生物合成路径中共同差异表达的基因有4个，分别是FAOMT、CCOAOMT、CYP73A4和HST。筛选出了与花青素合成相关结构基因CHI、ANS、F3H、PAL以及转录因子基因MYB113在萼下果皮表达差异显著。

关键词: 茄子, 转录组测序, 萼下果色, 光敏, 基因表达

Abstract:

In order to explore the different genes related to low light tolerance between the the photosensitive and non-photosensitive varieties，stable genetic purple under the calyx，round eggplant inbred line Y₅ and green under the calyx，round eggplant inbred line Y₇₃ were sequenced at three-time points（0，4 and 8 h）. The total effective data of 75.61 Gb was obtained，and the percentage of Q30 base reached 93.97%. 11 812 differential expression genes were obtained by sequencing and comparing two inbred lines at three-time points，including 5 277 up-regulated genes and 6 535 down-regulated genes. KEGG enrichment analysis of data at three time points shows three same pathways of phenylpropane biosynthesis，flavonoids biosynthesisand carbon fixation of photosynthetic organisms. There are four genes in the biosynthesis pathway of phenylpropane and flavonoids，named FAOMT，CCOAOMT，CYP73A4 and HST. The CHI，ANS，F3H，PAL genes and the transcription factor MYB113 gene related to anthocyanin synthesis were screened to express significantly in the peel under the calyx.

Key words: eggplant, transcriptome sequencing, color under the caylx, photosensitive, gene expression

中图分类号:

S641.1

乔军, 王利英, 刘婧, 李素文. 基于转录组测序的茄子萼下果色光敏相关基因表达分析[J]. 园艺学报, 2022, 49(11): 2347-2356.

QIAO Jun, WANG Liying, LIU Jing, LI Suweng. Expression Analysis of Genes Related to Photosensitive Color Under the Caylx in Eggplant Based on Transcriptome Sequencing[J]. Acta Horticulturae Sinica, 2022, 49(11): 2347-2356.

图/表 6

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样品 Sample	见光时间/h Light exposure time	重复 Repeat	过滤后碱基数/G Clean_bases	测序错误率/% Error_rate	Phred数值大于20的碱基百分比/% Q20	Phred数值大于30的碱基百分比/% Q30	过滤后reads中GC含量/% GC_pct
Y₇₃	0	1	3.08	0.0238	98.60	95.28	43.06
		2	4.41	0.0250	98.13	93.97	43.03
		3	3.63	0.0239	98.54	95.10	43.39
	4	1	2.28	0.0242	98.43	94.83	43.30
		2	3.75	0.0239	98.58	95.15	43.30
		3	5.14	0.0239	98.56	95.10	42.77
	8	1	3.54	0.0239	98.56	95.16	43.03
		2	3.84	0.0239	98.56	95.14	43.09
		3	3.73	0.0240	98.53	95.02	42.87
Y₅	0	1	3.97	0.0241	98.51	94.97	43.04
		2	3.15	0.0240	98.52	95.01	43.13
		3	4.26	0.0239	98.56	95.11	42.87
	4	1	5.63	0.0240	98.53	95.02	42.93
		2	4.89	0.0239	98.59	95.17	42.63
		3	5.93	0.0240	98.53	94.99	42.78
	8	1	5.52	0.0239	98.58	95.14	42.66
		2	4.42	0.0239	98.57	95.11	42.83
		3	4.44	0.0240	98.54	95.06	42.26
总计Total			75.61
平均Mean							42.94

样品 Sample	见光时间/h Light exposure time	重复 Repeat	过滤后碱基数/G Clean_bases	测序错误率/% Error_rate	Phred数值大于20的碱基百分比/% Q20	Phred数值大于30的碱基百分比/% Q30	过滤后reads中GC含量/% GC_pct
Y₇₃	0	1	3.08	0.0238	98.60	95.28	43.06
		2	4.41	0.0250	98.13	93.97	43.03
		3	3.63	0.0239	98.54	95.10	43.39
	4	1	2.28	0.0242	98.43	94.83	43.30
		2	3.75	0.0239	98.58	95.15	43.30
		3	5.14	0.0239	98.56	95.10	42.77
	8	1	3.54	0.0239	98.56	95.16	43.03
		2	3.84	0.0239	98.56	95.14	43.09
		3	3.73	0.0240	98.53	95.02	42.87
Y₅	0	1	3.97	0.0241	98.51	94.97	43.04
		2	3.15	0.0240	98.52	95.01	43.13
		3	4.26	0.0239	98.56	95.11	42.87
	4	1	5.63	0.0240	98.53	95.02	42.93
		2	4.89	0.0239	98.59	95.17	42.63
		3	5.93	0.0240	98.53	94.99	42.78
	8	1	5.52	0.0239	98.58	95.14	42.66
		2	4.42	0.0239	98.57	95.11	42.83
		3	4.44	0.0240	98.54	95.06	42.26
总计Total			75.61
平均Mean							42.94

样品 Sample	见光时间/h Light exposure time	重复 Repeat	有唯一比对位置的数量统计/% Unique_map	有多个比对位置的数量统计/% Multi_map	比对到两个外显子的数量统计/% Splice_map
Y₇₃	0	1	87.53	3.34	29.34
		2	88.44	3.70	32.32
		3	89.45	3.12	33.08
	4	1	85.85	2.93	28.61
		2	89.98	3.16	33.15
		3	89.76	3.21	31.95
	8	1	88.69	3.18	31.65
		2	87.88	3.41	31.60
		3	89.46	2.92	32.43
Y₅	0	1	89.72	3.15	31.67
		2	89.79	2.97	32.09
		3	89.02	3.24	31.20
	4	1	90.01	3.17	31.79
		2	89.74	3.13	31.07
		3	89.99	3.17	32.16
	8	1	89.82	3.08	32.31
		2	89.88	3.02	32.59
		3	88.85	3.12	30.48

样品 Sample	见光时间/h Light exposure time	重复 Repeat	有唯一比对位置的数量统计/% Unique_map	有多个比对位置的数量统计/% Multi_map	比对到两个外显子的数量统计/% Splice_map
Y₇₃	0	1	87.53	3.34	29.34
		2	88.44	3.70	32.32
		3	89.45	3.12	33.08
	4	1	85.85	2.93	28.61
		2	89.98	3.16	33.15
		3	89.76	3.21	31.95
	8	1	88.69	3.18	31.65
		2	87.88	3.41	31.60
		3	89.46	2.92	32.43
Y₅	0	1	89.72	3.15	31.67
		2	89.79	2.97	32.09
		3	89.02	3.24	31.20
	4	1	90.01	3.17	31.79
		2	89.74	3.13	31.07
		3	89.99	3.17	32.16
	8	1	89.82	3.08	32.31
		2	89.88	3.02	32.59
		3	88.85	3.12	30.48

见光时间/h Light exposure time	总数 All	上调 Up	下调 Down
0	2 545	1 060	1 485
4	4 578	2 179	2 399
8	4 689	2 038	2 651