关键词: 葡萄, 果皮, 蛋白质组, 双向电泳, 蔬菜十字花科, 硫苷, 光质, 分子机制

Abstract:

In order to study protein expression characteristics of grape pericarp in different coloring periods，early，middle and later stages of pericarp coloring were studied. 2-DE（Two-dimensional gel electrophoresis）and MALDI-TOF/TOF-MS（mass spectrometry technology）was used to analyze the differences of protein expression in pericarp at different coloring stages. (1) 2-DE revealed that there were 1 050 highly repeated protein spots during 3 periods of pericarp coloring，and 108 of 162 differential- expressed proteins were identified，87 proteins were mapped to the grape database. Twenty proteins differentially expressed during 3 periods of pericarp coloring，and the number of differential-expressed proteins was increasing with the deepening process of pericarp color. (2) GO enrichment analysis showed ATPβ（ATPase β subunit）was highly enriched in the ribose phosphate metabolic process，which was of great significance to the energy requirement of physiological metabolism in the early stage of coloring. (3) KEGG analysis showed that many KEGG pathways，such as carbon metabolism，photosynthetic carbon fixation，pentose phosphate，amino acid biosynthesis，metabolic pathways etc，were significantly enriched at different stages of pericarp coloration. (4) qRT-PCR analysis showed that the S-adenosylmethionine synthetase 4 gene（VvMETK4）had the highest expression level in the later stage of pericarp coloring.

Key words: Vitis vinifera, pericarp, proteome, 2-DE, vegetable, Cruciferae, glucosinolate, light quality, molecular mechanism