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园艺学报 ›› 2018, Vol. 45 ›› Issue (2): 333-.doi: 10.16420/j.issn.0513-353x.2017-0477

• 研究报告 • 上一篇    下一篇

MdSOS2L1基因苹果植株根际微生物多样性的研究

王晓娜,王小非,安建平,马齐军,胡大刚,周 波*,郝玉金*   

  1. 山东农业大学作物生物学国家重点实验室,山东泰安 271018
  • 出版日期:2018-02-25 发布日期:2018-02-25
  • 基金资助:

    国家自然科学基金项目(31601742);国家现代农业产业技术体系建设专项资金项目(CARS-28);山东省现代农业产业技术体系项目(SDAIT-06-03)

Studies on Rhizosphere Microbial Diversity of MdSOS2L1 Transgenic Apple

WANG Xiaona,WANG Xiaofei,AN Jianping,MA Qijun,HU Dagang,ZHOU Bo*,and HAO Yujin*   

  1. State Key Laboratory of Crop BiologyShandong Agricultural UniversityTai’anShandong 271018China
  • Online:2018-02-25 Published:2018-02-25

摘要:

以野生型‘嘎拉’苹果和转MdSOS2L1基因植株为试材,利用高通量测序技术研究转MdSOS2L1苹果植株对根际微生物的影响。结果表明,转基因与野生型植株根际微生物群落丰富度和多样性存在较大差异,其中原核微生物群落变化最为明显。转MdSOS2L1植株根际酸杆菌门丰度增加较多,蓝藻、放线菌门丰度略有下降。同时,转基因植株根际土壤pH值低于野生型,并且转基因植株根际土壤的苹果酸、柠檬酸、草酸含量明显升高,两者非根际土无明显差异,转基因植株根际微生物的差异极有可能与MdSOS2L1基因介导的有机酸分泌有关。

关键词: 苹果, MdSOS2L1, 根际微生物多样性, 高通量测序, 有机酸

Abstract:

Rhizosphere microorganisms have great effects on plant growth and development. The exudates of plants can also affect the distribution and species of microorganisms in rhizosphere. In this study,a high throughput sequencing technology was used to clarify the effects of MdSOS2L1 transgenic Gala apple plants on rhizosphere microorganisms. The results showed that there were great differences in the abundance and diversity of rhizosphere microorganisms among the MdSOS2L1 transgenic apple plants in compared with wild type apple plants. Among them,the rhizosphere of prokaryote community varied obviously in phylum level. The abundance of Acidobacteria in transgenic apple rhizosphere was higher than that in wild type apple plants. The abundance of Cyanobacteria and Actinobacteria was lower in transgenic apple rhizosphere microorganisms. Meanwhile,the pH value of rhizosphere soil of transgenic plants was lower than that of wild type plants. And the contents of oxalic acid,citric acid and L-malate in transgenic plants root were higher than that in wild type plants rhizosphere soil,but no significant differences occurred among the bulk soil between these two types of apple plants. Above of all,it is suggested that the MdSOS2L1-mediatedsecretion of organic acids contributes to the difference in rhizosphere microorganisms.

Key words: apple, MdSOS2L1, rhizosphere microbial diversity, high throughput sequencing, organic acids

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