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园艺学报 ›› 2022, Vol. 49 ›› Issue (5): 1008-1022.doi: 10.16420/j.issn.0513-353x.2021-0138

• 研究论文 • 上一篇    下一篇

不同类型桃种质资源主要酚类物质含量评价

蔡志翔1, 严娟1, 宿子文1, 徐子媛1, 张明昊2, 沈志军1, 杨军3, 马瑞娟1, 俞明亮1,*()   

  1. 1江苏省农业科学院果树研究所,江苏省高效园艺作物遗传改良重点实验室,南京 210014
    2上海市农业科学院林木果树研究所,上海 201403
    3江苏省农业科学院农业资源与环境研究所,南京 210014
  • 收稿日期:2021-11-10 修回日期:2022-01-20 出版日期:2022-05-25 发布日期:2022-05-25
  • 通讯作者: 俞明亮 E-mail:mly1008@aliyun.com
  • 基金资助:
    江苏省自然科学基金项目(BK20200278);国家重点研发计划项目(2019YFD1000200);农业农村部物种保护项目(19210137);国家科技资源共享服务平台项目(NHGRC2021-NH16);江苏省种业揭榜挂帅项目(JBGS(2021)016)

Evaluation of Main Phenolic Compounds in Different Types of Peach Germplasm Resources

CAI Zhixiang1, YAN Juan1, SU Ziwen1, XU Ziyuan1, ZHANG Minghao2, SHEN Zhijun1, YANG Jun3, MA Ruijuan1, YU Mingliang1,*()   

  1. 1Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement,Institute of Pomology,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China
    2Forest and Fruit Tree Research Institute,Shanghai Academy of Agricultural Sciences,Shanghai 201403,China
    3Institute of Agricultural Resources and Environment,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China
  • Received:2021-11-10 Revised:2022-01-20 Online:2022-05-25 Published:2022-05-25
  • Contact: YU Mingliang E-mail:mly1008@aliyun.com

摘要:

以257份桃种质成熟期果实为试材,利用高效液相色谱法(HPLC)测定果肉中6种酚类物质和3种有机酸含量,分析比较不同种质果肉单体酚和总酚含量的差异,并讨论不同肉色、肉质和有机酸含量的种质类型间酚类物质的含量差异。结果显示,桃果肉中总酚含量区间为31.05 ~ 919.11 mg · kg-1 FW,其中绿原酸、新绿原酸、儿茶素含量较高,平均值分别为56.60、43.20和25.79 mg · kg-1 FW,表儿茶素、芦丁含量中等,平均值分别为14.77和14.85 mg · kg-1 FW,阿魏酸含量最低(1.57 mg · kg-1 FW);筛选到单体酚和总酚含量特异种质11份;不同肉色类型桃的主要酚类物质含量存在差异,红肉类型显著高于白肉和黄肉类型,黄肉与白肉类型差异不大;不同肉质类型桃主要酚类物质含量为硬肉显著高于溶质和硬质类型(P < 0.05);果实中苹果酸、柠檬酸、奎尼酸含量均与总酚含量呈极显著正相关。

关键词: 桃, 种质资源, 酚类物质, 有机酸, 肉色, 肉质

Abstract:

The contents of six phenolic compounds and three organic acids in pulp of 257 peach germplasms were determined by High-Performance Liquid Chromatography(HPLC),the mono-phenol and total phenol contents in different germplasms were compared,and their contents in different types of germplasm resources with different pulp color,pulp texture and organic acid content were discussed. The results showed that the contents of total phenol in peach pulp ranged from 31.05 to 919.11 mg · kg-1 FW,among which the contents of chlorogenic acid,neochlorogenic acid and catechin were highest,the mean values were 56.60,43.20 and 25.79 mg · kg-1 FW,respectively,the average contents of epicatechin and rutin were 14.77 and 14.85 mg · kg-1 FW,respectively,and the content of ferulic acid was lowest with 1.57 mg · kg-1 FW. Eleven special varieties were selected according to the mono-phenol and total phenol contents. In different types of pulp color,the total phenol content in red pulp were significantly higher than those in white and yellow pulp(P < 0.05),the total phenol content of yellow pulp was no significant difference with white pulp. In different types of pulp texture,the phenolics content of crisp peach were significantly higher than in melting and stony hard peach(P < 0.05). The total phenol content was significantly correlated with the contents of malic acid,citric acid and quinic acid in pulp.

Key words: peach, germplasm resource, phenolic compounds, organic acid, pulp color, pulp textrue

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