园艺学报 ›› 2016, Vol. 43 ›› Issue (10): 2049-2058.doi: 10.16420/j.issn.0513-353x.2016-0307

• 研究报告 • 上一篇    下一篇


顾 绘1,2,陈赛男1,李良俊1,*,程立宝1   

  1. (1扬州大学园艺与植物保护学院,江苏扬州 225009;2南通科技职业学院,江苏南通 226007)
  • 出版日期:2016-10-25 发布日期:2016-10-25

Cloning and Expression Analysis of Starch Branching Enzyme Gene in Taro(Colocasia esculenta)

GU Hui1,2,CHEN Sai-nan1,LI Liang-jun1,*,and CHENG Li-bao1   

  1. (1School of Horticulture and Plant Protection,Yangzhou University,Yangzhou,Jiangsu 225009,China;2Nantong Science and Technology College,Nantong,Jiangsu 226007,China)
  • Online:2016-10-25 Published:2016-10-25


以多子芋品种‘香堂芋’幼嫩叶片为试材,利用RACE结合RT-PCR技术克隆得到全长为3 051 bp的淀粉分支酶SBE基因cDNA序列(登录号:KX013544),其中开放阅读框长2 538 bp,编码845个氨基酸;该序列与玉米SBEII、马铃薯SBEIIa同源性均达85%以上;芋SBE编码蛋白具有N–末端、C–末端和α–淀粉酶催化域。利用RT-PCR技术克隆得到芋SBE的基因组DNA序列,全长为12 362 bp,包括20个外显子和19个内含子。芋球茎形成过程中,子芋植株叶片中SBE表达量显著高于母芋植株叶片、叶柄和根系及母芋等组织,子芋膨大过程中SBE表达量逐渐升高,而孙芋膨大过程中SBE表达量逐渐下降。子芋、孙芋SBE表达对支链淀粉合成积累可能起重要作用。

关键词: 芋, 淀粉分支酶基因, 克隆, 表达


In this study,a soluble starch branching gene(SBE)was isolated from the leaf of ‘Xiangtangyu’,a species of taro based on RT-PCR and PCR-RACE methods. The full-length of SBE cDNA(GenBank accession number KX013544)was 3 051 bp in nucleotide containing an open reading frame of 2 538 bp which encoding 845 amino acids. Phylogenetic analysis showed that SBE had more than 85% homolog with maize,potato and rice,respectively. SBE protein contained N-terminal end,C-terminal end and alpha amylase catalytic domain. DNA sequence of SBE was cloned by RT-PCR method,and the full length of DNA was 12 362 bp with 20 exons and 19 introns. During corm formation the expression of SBE in leaf of son-taro plants was significantly higher than that of mother-taro plants,leafstalks,roots,mother-taro and so on,the expression was improved in son-taros,and decreased in grade-taros with development of corm. Totally,we believed that the expression of SBE was synchronous with amylopectin synthesis in son-taros and grade-taros.

Key words: Colocasia esculenta, starch branching gene, clone, expression