苹果果实β-Gal和LOX活性变化特性及其与果实软化的关系

园艺学报 ›› 2009, Vol. 36 ›› Issue (5): 631-638.

苹果果实β-Gal和LOX活性变化特性及其与果实软化的关系

魏建梅^1,2;马锋旺^1*

(¹西北农林科技大学园艺学院, 陕西杨凌712100; ²河北省农林科学院昌黎果树研究所, 河北昌黎066600)

收稿日期:2008-10-27 修回日期:2009-01-22 出版日期:2009-05-25 发布日期:2009-05-25
通讯作者: 马锋旺

The Character istics of β-Gal and LOX Activities in Apple (Malus domestica Borkh. ) Fruit and Their Relation to Fruit Softening

WEI Jian-mei^1,2 and MA Feng-wang^1*

(¹College of Horticulture, N orthwestA & F University, Yangling, Shaanx i 712100, China; ²Changli Fruit Institute, Hebei Academy of Agriculture and Forestry Sciences, Changli, Hebei 066600, China)

Received:2008-10-27 Revised:2009-01-22 Online:2009-05-25 Published:2009-05-25
Contact: MA Feng-wang

摘要/Abstract

摘要： 以‘富士’和‘金冠’苹果果实为试材, 研究了β-半乳糖苷酶(β-Gal) 和脂氧合酶(LOX) 在果实发育、成熟和软化过程中的变化规律及采后乙烯调控对其活性的影响。结果表明: 在果实
发育后熟过程中, 金冠果实β-Gal活性显著高于富士, 在果实后熟软化期间这种差异尤为突出; 而富士和金冠果实LOX活性呈相似的变化规律, 虽然花后富士的活性高峰显著高于金冠, 但之后其活性下降迅速,并一直低于金冠。采后苹果果实β-Gal和LOX均受乙烯调控, 乙烯抑制剂1-MCP极显著地抑制β-Gal和LOX的活性, 而乙烯利对二者活性起促进作用, 但因品种耐藏性不同其促进效应不同。综合来看, 苹果果实β-Gal和LOX活性表现相似的变化规律, 并且β-Gal在果实贮藏初期受乙烯的调控作用较LOX显著, 因此认为在苹果果实软化早期β-Gal的作用可能大于LOX。

关键词: 苹果, 采后, β-半乳糖苷酶, 脂氧合酶, 活性, 乙烯调控

Abstract: For understanding the role of β-galactosidase (β-Gal) and lipoxygenase (LOX) during fruit development, ripening and softening, the fruits of app le (Malus domestica Borkh. ) cultivars, ‘Fuji’and‘Golden Delicious’, were used to investigate the activity changes of β-Gal and LOX over this period, and
during postharvest regulation by ethylene. The results indicated that theβ2Gal activity in‘Golden Delicious’was markedly higher than that in‘Fuji’during fruit development and ripening and the difference was in particular significant during ripening and softening. The LOX activity in‘Fuji’was dramatically increased and higher than that in‘Golden Delicious’after flowering, but its activity wasmarkedly reduced and maintained lower than that of‘Golden Delicious’before fruit ripening. The β-Gal and LOX activities of app le fruitwere
markedly inhibited by 12methycyclopene ( 1-MCP) and increased by ethephon. The changes of β-Gal and LOX activity showed a similar trend and the β-Gal be much easier to be regulated by ethylene than LOX.These results suggested that the change of β-Gal and LOX activities be correlated with the storage characteristic of apple fruit and β-Galmight have an important role in the early stage of fruit softening.

Key words: apple, postharvest, β-Gal, LOX, activity, ethylene regulation

中图分类号:

S 661.1

魏建梅;马锋旺. 苹果果实β-Gal和LOX活性变化特性及其与果实软化的关系[J]. 园艺学报, 2009, 36(5): 631-638.

WEI Jian-mei;and MA Feng-wang. The Character istics of β-Gal and LOX Activities in Apple (Malus domestica Borkh. ) Fruit and Their Relation to Fruit Softening[J]. ACTA HORTICULTURAE SINICA, 2009, 36(5): 631-638.

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