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园艺学报 ›› 2008, Vol. 35 ›› Issue (1): 47-52.

• 蔬菜 • 上一篇    下一篇

番茄耐热性相关的SSR和RAPD标记筛选

许向阳;王冬梅;康立功;李景富*   

  1. (东北农业大学园艺学院, 哈尔滨150030)
  • 收稿日期:2007-05-13 修回日期:2007-09-17 出版日期:2008-01-25 发布日期:2008-01-25
  • 通讯作者: 李景富

Selection of SSR and RAPD Markers Related to Tomato Heat Tolerance

Xu Xiang-yang, Wang Dong-mei, Kang Li-gong, and Li Jing-fu*   

  1. (Horticulture College, Northeast Agricultural University, Harbin 150030, China)
  • Received:2007-05-13 Revised:2007-09-17 Online:2008-01-25 Published:2008-01-25

摘要: 以耐热性差异较大的热敏感材料 01137和耐热材料CLN2001A杂交产生的256个F2单株为作图群体,应用SSR和RAPD 分子标记技术筛选了与番茄耐热性数量性状相关的分子标记,得到与耐热性相关的1个SSR标记和1个RAPD标记,对其耐热性遗传的贡献率分别为5.2%和7.7%。利用单标记法在第3 和第7连锁群上各检测到1个与耐热性有关的QTL。利用筛选出的与番茄耐热性显著相关2个分子标记对43份番茄耐热、热敏种质资源进行SSR和RAPD分析,将43份番茄种质资源分成2组,耐热组的分组准确率为93.3%,热敏组为96.5%。

关键词: 番茄, 耐热性, 数量性状位点(QTL)

Abstract: A F2 population was derived from a cross between a heat sensitive line 01137 with a heat tolerance line CLN2001A of tomato. The colony was analyzed using SSR and RAPD techniques to select molecular markers related to tomato heat tolerance. One SSR marker and one RAPD marker were detected, and the genetic contribution rate of these markers for heat tolerance is 5.2% and 7.7% respectively. Two QTLs were mapped in linkage group 3 and linkage group 7. The SSR and RAPD techniques were used to evaluate the genetic diversity of 43 tomato germplasm materials about heat resistance and susceptibility. we use two markers(Tom59-60 and OPN02-600)which were significantly interrelated to the heat-tolerance of tomato to group the 43 tomato germplasm materials under two groups, the heat-tolerance and the heat-sensitive, and the veracity of grouping were 93.3% and 96.5% respectively.

Key words: tomato, heat tolerance, quantitative trait loci(QTL)

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