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园艺学报 ›› 2022, Vol. 49 ›› Issue (8): 1785-1794.doi: 10.16420/j.issn.0513-353x.2021-0418

• 研究报告 • 上一篇    下一篇

海南西番莲主要病毒种类的分子检测与鉴定

陈礼浪1, 杨天章1, 蔡儒平1, 林小漫1, 邓南康1, 车海彦2, 林雅婷2, 孔祥义1,*()   

  1. 1.三亚市热带农业科学研究院,海南三亚 572000
    2.中国热带农业科学院环境与植物保护研究所,海口 571101
  • 收稿日期:2022-04-14 修回日期:2022-08-16 出版日期:2022-08-25 发布日期:2022-09-05
  • 通讯作者: 孔祥义 E-mail:kongxiangyi20@163.com
  • 基金资助:
    三亚市农业科技创新项目(2019NKO7);海南省重大科技计划项目(ZDKJ202002)

Molecular Detection and Identification of Viruses from Passiflora edulis in Hainan

CHEN Lilang1, YANG Tianzhang1, CAI Ruping1, LIN Xiaoman1, DENG Nankang1, CHE Haiyan2, LIN Yating2, KONG Xiangyi1,*()   

  1. 1. Sanya Academy of Tropical Agricultural Sciences,Sanya,Hainan 572000,China
    2. Environment and Plant Protection Institute,Chinese Academy of Tropical Agricultural Sciences,Haikou 571101,China
  • Received:2022-04-14 Revised:2022-08-16 Online:2022-08-25 Published:2022-09-05
  • Contact: KONG Xiangyi E-mail:kongxiangyi20@163.com

摘要:

2020年5—7月,在海南三亚市和五指山市采集表现花叶、皱缩、畸形等疑似病毒病的西番莲叶片样品176份,通过PCR和RT-PCR检测发现:侵染西番莲的病毒主要为黄瓜花叶病毒(cucumber mosaic virus,CMV)、南瓜蚜传黄化病毒(cucurbit aphid-borne yellows virus,CABYV)、东亚西番莲病毒(East Asian Passiflora virus,EAPV)、夜来香花叶病毒(Telosma mosaic virus,TeMV)、西番莲木质化病毒(passion fruit woodiness virus,PWV)、西番莲斑驳病毒(Passiflora mottle virus,PaMV)和番茄黄化曲叶病毒(tomato yellow leaf curl virus,TYLCV)。TeMV的检出率最高,为52.27%;其次为PaMV,43.75%;CMV和EAPV均为28.98%;PWV为22.73%;TYLCV为9.09%;CABYV为4.55%。TeMV、PaMV、CMV、EAPV和PWV可能是三亚和五指山西番莲的主要病毒,样品病毒复合侵染率为78.91%,存在32种复合侵染类型,以2 ~ 4种病毒复合侵染为主。3个TYLCV西番莲分离物的基因组全长均为2 781 bp,含6个ORF。序列相似性和系统进化分析结果表明:3个分离物均属于TYLCV-Israel株系,序列相似性为98.85% ~ 99.14%,与海南已报道的4个其他作物上的分离物聚在同一进化分支上。这是TYLCV侵染西番莲的首次报道。

关键词: 西番莲, 病毒病, 病原检测, 番茄黄化曲叶病毒

Abstract:

A total of 176 Passiflora edulis leaf samples showing mosaic,crinkle and deformation were collected from Sanya and Wuzhishan of Hainan Province from May to July 2020. Seven viruses were detected by PCR and RT-PCR including cucumber mosaic virus ( CMV ),cucurbit aphid-borne yellows virus ( CABYV ),East Asian Passiflora virus ( EAPV ),Telosma mosaic virus ( TeMV ),passion fruit woodiness virus ( PWV ),Passiflora mottle virus ( PaMV ) and tomato yellow leaf curl virus ( TYLCV ). The result showed that TeMV was the most prevalent virus with infection rate of 52.27%,followed by PaMV(43.75%),CMV and EAPV ( 28.98% ),PWV ( 22.73% ),TYLCV ( 9.09% ) and CABYV ( 4.55% ). TeMV,PaMV,CMV,EAPV and PWV may be the main viruses infecting P. edulis in Sanya and Wuzhishan. 78.91% of samples were detected co-infection with a total of 32 kinds of co-infection types,in which co-infection of two to four viruses commonly occurs. Three complete genome sequences of TYLCV from P. edulis were determined to be 2 781 bp and encoded six open reading frames ( ORFs ). Both the identity and phylogenetic analysis based on the complete genome showed that all the three TYLCV isolates belonged to TYLCV Israel strain with nt identity ranging from 98.85% to 99.14%,and clustered in the same branch with the four TYLCV isolates from other crops in Hainan. To our knowledge,this is the first report of TYLCV in P. edulis.

Key words: Passiflora edulis, virus disease, pathogen detection, tomato yellow leaf curl virus

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