茉莉酸合成基因LoxD参与调控番茄的抗旱性

doi:10.16420/j.issn.0513-353x.2021-0025

摘要/Abstract

摘要：

为了研究茉莉酸（JA）调节番茄抗旱的机理,以番茄品种‘Castlemart’茉莉酸合成基因LoxD过表达植株（LoxD-oe）、LoxD点突变植株（spr8）以及对应的野生型（WT）为材料,进行自然失水处理,分析叶片相对含水量、可溶性糖、丙二醛含量、气孔开度和保卫细胞中H₂O₂含量,以及ABA信号通路和质膜相关离子通道基因等表达的变化。结果表明,LoxD受干旱胁迫诱导,过表达LoxD基因提高了叶片相对含水量和可溶性糖含量,降低了MDA的含量,上调了SnRK2-like、SLAC1、AtrbohD、CPK1表达,下调ABI2、KAT1表达,激活了保卫细胞中H₂O₂信号,气孔开度变小,从而减少了干旱胁迫下水分的散失。说明LoxD基因参与了番茄抗旱性的调控。

关键词: 番茄, 茉莉酸, 干旱胁迫, LoxD基因, 气孔运动, H₂O₂荧光信号

Abstract:

To study the drought resistance mechanism regulated by JA,using tomato variety‘Castlemart’jasmonic acid synthesis gene LoxD-overexpression transgenic plants（LoxD-oe）and LoxD mutant（spr8）and their corresponding wild type（WT）as material. Under the drought stress,the relative water content,soluble sugar,malondialdehyde content,stomatal aperture,H₂O₂ level in guard cells,and the expressions of the ABA signaling and related ion channel genes were determined. It was found that LoxD gene was induced by drought stress. The overexpression of LoxD gene gave rise to the increases of the relative water content and soluble sugar content and the decline of MDA production in leaves. Moreover,under drought stress,overexpressing LoxD gene induced the expressions of SnRK2-like,SLAC1,AtrbohD and CPK1,inhibited the expressions of ABI2 and KAT1,and activated the H₂O₂ signal in guard cells,which probably arrested the stomatal opening and then reduced the water loss. The results showed that LoxD gene participates in regulating drought resistance.

Key words: tomato, jasmonic acid, drought stress, LoxD gene, stomatal movement, H₂O₂ fluorescence signal

中图分类号:

S641.2

彭轶, 李元慧, 杨瑞, 张子怡, 李亚楠, 韩云昊, 赵文超, 王绍辉. 茉莉酸合成基因LoxD参与调控番茄的抗旱性[J]. 园艺学报, 2022, 49(2): 319-331.

PENG Yi, LI Yuanhui, YANG Rui, ZHANG Ziyi, LI Yanan, HAN Yunhao, ZHAO Wenchao, WANG Shaohui. The Jasmonic Acid Synthesis Gene LoxD Participates in the Regulation of Tomato Drought Resistance[J]. Acta Horticulturae Sinica, 2022, 49(2): 319-331.

图/表 10

表1 qRT-PCR引物序列

Table 1 Primer sequences for qRT-PCR

基因 Gene	LOC编号 LOC ID	上游引物（5′-3′） Forward primer	下游引物（5′-3′） Reverse primer
LoxD	Solyc03g122340	TCCATAACTTAATTCCATCTC	TGATAGTGCTAACAACCT
LoxC	Solyc01g006540	GAGCTAACTAGGCCACCAATAG	CCGGAGTCATGAGAAAGAACA
AOS1	Solyc04g079730	CATCATCATCGTCATCAC	GAAGTAATCAAGTCTGTCTT
AOC	Solyc02g085730	CTATCTTCTGCCTTCCAA	TGTTAGTTGAATCTGTTGAG
SnRK2-like	Solyc01g108280	TGGGACCAACAGCCTTAATC	GGTCAGGATCACTCTCCAAATC
SLAC1 AtrbohD	Solyc08g079770.2 Solyc03g117980	GCAGAGGAGGTTCCATCTTTC TACTGTCCCACTAGCACTCTAT	GCAGAAGGGTTGAGACGAATAA GTGAAGGGCCAACACATTTC
CPK1	Solyc03g031670	GAACTCAACTGGGAACCCTTAT	TCCCTCTTCATATCCTCCCTATC
ABI2	Solyc03g121880	CAGACTGTATTGGGAGCTGAAA	CATGGATCCTCTCCAACAGATAAA
KAT1	Solyc02g070530	GACCAGATGAGGGAACAAAGAG	GCCACTTGAACTGGTACTACAT

图1 干旱胁迫下番茄JA合成基因的转录应答

Fig. 1 Transcriptional response of tomato JA synthesis genes under drought stress

图2 干旱胁迫下MeJA对番茄野生型（WT）抗旱性的影响

Fig. 2 Effect of MeJA on drought resistance of wild-type （WT）tomato under drought stress

图3 番茄野生型（WT）、LoxD-oe、spr8株系在干旱处理下的表型变化

Fig. 3 Phenotype of wild-type（WT）, LoxD-oe,spr8 tomato plants under drought treatment

图4 干旱胁迫下WT,LoxD-oe和spr8叶片相对含水量（A）、可溶性糖（B）和丙二醛含量（C）的变化

Fig. 4 Relative water content（A）,soluble sugar content（B）and malondialdehyde content（C）of WT, LoxD-oe and spr8 tomatoes under drought stress P < 0.05.

图5 番茄野生型WT、LoxD-oe、spr8在干旱胁迫下叶片下表皮气孔开度统计（A）和气孔观察（B）

Fig. 5 Stomatal aperture of WT, LoxD-oe,spr8 leaves under drought stress

表2 番茄WT、LoxD-oe、spr8在干旱胁迫下叶片下表皮气孔张开比例

Table 2 Percentage statistics of stomata opening in the epidermis of tomato WT,LoxD-oe and spr8 under drought stress

处理 Treatment	番茄 Tomato	开放/% Opening
对照 Control	WT	83.19
	LoxD-oe	67.44
	spr8	84.21
干旱 Drought	WT	56.41
	LoxD-oe	24.63
	spr8	65.71

图6 番茄野生型WT外源喷施MeJA叶片表皮保卫细胞胞质中H2O2的荧光图像

Fig. 6 Levels of H2O2 generation from guard cells of WT（spraying 50 μmol · L-1 MeJA）

图7 干旱胁迫下不同番茄株系叶片表皮保卫细胞胞质中H2O2的荧光图像

Fig. 7 Levels of H2O2 generation from guard cells of WT,LoxD-oe,spr8（under drought stress）

图8 干旱胁迫下保卫细胞气孔相关通路基因的转录水平

Fig. 8 Transcription level of guard cell stomatal pathway genes under drought stress P < 0.05.

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