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园艺学报 ›› 2008, Vol. 35 ›› Issue (1): 53-58.

• 蔬菜 • 上一篇    下一篇

利用分子标记对辣椒抗马铃薯Y病毒的3个QTLs进行选择

王立浩1; 张宝玺1*;C. Caranta2;毛胜利1; A. Palloix2*   

  1. 1中国农业科学院蔬菜花卉研究所,北京100081;2法国农业科学院蔬菜与果树遗传改良中心, 法国蒙发威,BP 94 F-84143)
  • 收稿日期:2007-08-19 修回日期:2007-10-19 出版日期:2008-01-25 发布日期:2008-01-25
  • 通讯作者: 张宝玺

Molecular Markers Assisted Selection for Three QTLs Resistant to PVY in Pepper(Capsicum annuum L.)

WANG Li-hao1, ZHANG Bao-xi1*, C. Caranta2, MAO Sheng-li1, and A. Palloix2*   

  1. 1Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China; 2INRA, Unité de Génétique et Amélioration des Fruits et Légumes, BP 94, F-84143 Montfavet cedex, France)
  • Received:2007-08-19 Revised:2007-10-19 Online:2008-01-25 Published:2008-01-25

摘要: 为获得含有特定基因型的个体,深入研究辣椒抗马铃薯Y病毒(PVY)不同QTLs之间的关系,利用AFLP、SCAR和CAPS标记在辣椒材料‘Perennial’和‘Yolo Wonder’组成的BC2和BC1S1分离世代对抗PVY的3个QTLs位点进行了选择。结果从BC2群体中选出47个在主效QTL上含有抗性位点的单株,从中选出25个在LG9 PY2染色体上含有抗性位点的单株,最后选出11个在LG11 P10染色体上含有抗性位点的单株;从BC1S1群体中选出55个在LG9 PY2染色体上具有纯合抗性位点的单株,从中选出12个在主效QTL上纯合抗性位点的单株。经卡平方检测,实际分离比例同理论分离比例差异不显著。

关键词: 辣椒, 马铃薯Y病毒, 分子标记辅助选择, QTLs

Abstract: Markers assisted selection is still seldom used in pepper breeding in China, though many markers are reported recently. In order to obtain genotypes for researching of relationship of QTLs resistant to PVY, AFLP, CAPS, SCAR markers were used to assist selection of QTLs for resistance to PVY in BC2 and BC1S1 segregating progenies from pepper 'perennial' and 'Yolo Wonder'. With the markers, 47 plants with resistant allele in major QTL locus, then from which 25 plants with resistant allele in LG9 PY2, at last 11 plants with resistant allele in LG11 P10 were selected from the BC2 population. And 55 plants with homozygous resistant allele in LG9 PY2, and 12 plants with homozygous resistant allele in major QTL were selected from the BC1S1 population. X2 analysis showed that the segregating ratio in fact is not significantly different with that in theory.

Key words: Capsicum annuum L., pepper, PVY, markers assisted selection, QTLs

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