瓜类作物CRISPR/Cas9基因编辑技术应用研究进展

doi:10.16420/j.issn.0513-353x.2023-0503

摘要/Abstract

摘要：

主要概述了CRISPR/Cas9基因编辑技术的来源、分类以及作用机制，总结归纳了 CRISPR/Cas9技术在瓜类作物基因功能鉴定和性状改良上的研究进展，讨论了在瓜类作物中基因编辑技术应用的难点和解决方法，以期为基因编辑技术在瓜类作物中广泛应用提供参考。

关键词: 葫芦科, 基因编辑, CRISPR/Cas9, 性状改良, 研究进展

Abstract:

It mainly outlines the origin，classification and mechanism of CRISPR/Cas9 gene editing technology，summarizes the research progress of CRISPR/Cas9 technology in the identification of gene function and trait improvement in melon crops，and discusses the difficulties and solutions for the application of gene editing technology in melon crops，so as to provide a reference for the wide application of gene editing technology in melon crops.

Key words: Cucurbitaceae, gene editing, CRISPR/Cas9, character improvement, research progress

江睿, 周胜军, 朱育强, 王欣, 谭继宏, 王华森, 张鹏. 瓜类作物CRISPR/Cas9基因编辑技术应用研究进展[J]. 园艺学报, 2024, 51(7): 1683-1694.

JIANG Rui, ZHOU Shengjun, ZHU Yuqiang, WANG Xin, TAN Jihong, WANG Huasen, ZHANG Peng. Research Progress of CRISPR/Cas9 Gene Editing Techniques in Melon Crops[J]. Acta Horticulturae Sinica, 2024, 51(7): 1683-1694.

图/表 1

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植物 Plant	基因 Gene	基因功能 Gene function	参考文献 Reference
黄瓜 Cucumis sativus	eIF4E	调控植株抗病毒性Regulation of plant virus resistance	Chandrasekaran et al.，2016
	WIP1	抑制心皮发育Inhibition of plant carpel development	Hu et al.，2017
	CsVFB1	调控植株株高Regulation of plant height	戚晶晶，2017
	CsWIP1	抑制心皮发育Inhibition of plant carpel development	杨丽，2018
	FT	调控花期及高纬度适应性Controlling blooming season and high latitude adaptation	Wang et al.，2020
	TEN	调控乙烯合成，控制卷须的形态和攀援Regulation of ethylene synthesis and control of tendril morphology and climbing	Yang et al.，2020
	ACS1G	雌花发育关键基因Key genes for female flower development	Zhang et al.，2021
	Bcmfs1	拮抗灰霉病菌Antagonism of grey mould	Samaras et al.，2021
	CsHEC2，CsGL3，CsTu	调控果实刺瘤密度Regulation of fruit prickly tumour density	Wang et al.，2021
	NS	调控果实多刺Regulation of fruit multispinning	Liu et al.，2022
	CsIAGLU	调控叶柄角度Regulation of petiole angle	Chen et al.，2022
	Csalc	调控黄瓜雌蕊育性Regulation of cucumber pistil fertility	Cheng et al.，2023
	CsPRE4	调控黄瓜卷须伸长Regulation of cucumber tendril elongation	Xu et al.，2023
西瓜 Citrullus lanatus	PDS	调控植株白化Regulation of plant whitening	Tian et al.，2017
	ALS	抗苯磺隆类除草剂Bensulfuron-resistant herbicides	Tian et al.，2018
	ClWIP1	调控性别分化Regulation of gender differentiation	张月乔等，2020
	Cldf	调控西瓜矮化Regulation of watermelon dwarfing	Wei et al.，2019
	Clpsk1	调控西瓜抗枯萎病Regulation of blight resistance in watermelon	Zhang et al.，2020
	ClSPO11-1	诱导产生不定根Inducing adventitious root production	Zhang et al.，2020
	ClCOMT1	调控植物内源褪黑素含量，参与多种非生物胁迫响应 Regulation of endogenous melatonin content in plants and involved in multiple abiotic stress responses	Chang et al.，2021
	ClGRF4-GIFl	优化和改造基因编辑体系，二倍体无籽西瓜Optimisation and modification of gene editing systems for diploid seedless watermelon	Feng et al.，2021
	ClATM1	调控花期及雄性不育Regulation of flowering time and male sterility	Zhang et al.，2021
	GRF5，PDS	建立西瓜遗传转化体系及突变体快速获取Establishment of watermelon genetic transformation system and rapid acquisition of watermelon mutants	Pan et al.，2022
甜瓜 Cucumis melo	ACC	抑制乙烯的生物合成及调控果实软化 Inhibition of ethylene biosynthesis and regulation of fruit softening	王雪和李冠，2017
	eIF4E	调控植株抗病毒性Regulation of plant virus resistance	杨晶等，2018；Huang et al.，2019
	PLL	调控植株叶形Regulation of plant leaf shape	王丹等，2018
	CmPDS	调控植株白化Regulation of plant whitening	Hooghvorst et al.，2019
	CTR1，ROS1	果实成熟和表观遗传调控Fruit ripening and epigenetic regulation	Giordano et al.，2022
	CmACS1，CmACS2，CmACS5	调控果实软化Regulation of fruit softening	颜清成等，2022
	ER	调控茎伸长Regulation of stem elongation	Xin et al.，2022
	AMS	调控雄性不育Regulation of male sterility	才羿等，2023
南瓜Cucurbita moschata	RBOHD	调控耐盐性Regulation of salt tolerance	Huang et al.，2019
南瓜Cucurbita moschata	ER	调控茎伸长Regulation of stem elongation	Xin et al.，2022
丝瓜Luffa aegyptiaca Miller	LcPPO1	调控果实褐变Regulation of fruit browning	陈敏氡等，2021

植物 Plant	基因 Gene	基因功能 Gene function	参考文献 Reference
黄瓜 Cucumis sativus	eIF4E	调控植株抗病毒性Regulation of plant virus resistance	Chandrasekaran et al.，2016
	WIP1	抑制心皮发育Inhibition of plant carpel development	Hu et al.，2017
	CsVFB1	调控植株株高Regulation of plant height	戚晶晶，2017
	CsWIP1	抑制心皮发育Inhibition of plant carpel development	杨丽，2018
	FT	调控花期及高纬度适应性Controlling blooming season and high latitude adaptation	Wang et al.，2020
	TEN	调控乙烯合成，控制卷须的形态和攀援Regulation of ethylene synthesis and control of tendril morphology and climbing	Yang et al.，2020
	ACS1G	雌花发育关键基因Key genes for female flower development	Zhang et al.，2021
	Bcmfs1	拮抗灰霉病菌Antagonism of grey mould	Samaras et al.，2021
	CsHEC2，CsGL3，CsTu	调控果实刺瘤密度Regulation of fruit prickly tumour density	Wang et al.，2021
	NS	调控果实多刺Regulation of fruit multispinning	Liu et al.，2022
	CsIAGLU	调控叶柄角度Regulation of petiole angle	Chen et al.，2022
	Csalc	调控黄瓜雌蕊育性Regulation of cucumber pistil fertility	Cheng et al.，2023
	CsPRE4	调控黄瓜卷须伸长Regulation of cucumber tendril elongation	Xu et al.，2023
西瓜 Citrullus lanatus	PDS	调控植株白化Regulation of plant whitening	Tian et al.，2017
	ALS	抗苯磺隆类除草剂Bensulfuron-resistant herbicides	Tian et al.，2018
	ClWIP1	调控性别分化Regulation of gender differentiation	张月乔等，2020
	Cldf	调控西瓜矮化Regulation of watermelon dwarfing	Wei et al.，2019
	Clpsk1	调控西瓜抗枯萎病Regulation of blight resistance in watermelon	Zhang et al.，2020
	ClSPO11-1	诱导产生不定根Inducing adventitious root production	Zhang et al.，2020
	ClCOMT1	调控植物内源褪黑素含量，参与多种非生物胁迫响应 Regulation of endogenous melatonin content in plants and involved in multiple abiotic stress responses	Chang et al.，2021
	ClGRF4-GIFl	优化和改造基因编辑体系，二倍体无籽西瓜Optimisation and modification of gene editing systems for diploid seedless watermelon	Feng et al.，2021
	ClATM1	调控花期及雄性不育Regulation of flowering time and male sterility	Zhang et al.，2021
	GRF5，PDS	建立西瓜遗传转化体系及突变体快速获取Establishment of watermelon genetic transformation system and rapid acquisition of watermelon mutants	Pan et al.，2022
甜瓜 Cucumis melo	ACC	抑制乙烯的生物合成及调控果实软化 Inhibition of ethylene biosynthesis and regulation of fruit softening	王雪和李冠，2017
	eIF4E	调控植株抗病毒性Regulation of plant virus resistance	杨晶等，2018；Huang et al.，2019
	PLL	调控植株叶形Regulation of plant leaf shape	王丹等，2018
	CmPDS	调控植株白化Regulation of plant whitening	Hooghvorst et al.，2019
	CTR1，ROS1	果实成熟和表观遗传调控Fruit ripening and epigenetic regulation	Giordano et al.，2022
	CmACS1，CmACS2，CmACS5	调控果实软化Regulation of fruit softening	颜清成等，2022
	ER	调控茎伸长Regulation of stem elongation	Xin et al.，2022
	AMS	调控雄性不育Regulation of male sterility	才羿等，2023
南瓜Cucurbita moschata	RBOHD	调控耐盐性Regulation of salt tolerance	Huang et al.，2019
南瓜Cucurbita moschata	ER	调控茎伸长Regulation of stem elongation	Xin et al.，2022
丝瓜Luffa aegyptiaca Miller	LcPPO1	调控果实褐变Regulation of fruit browning	陈敏氡等，2021