m6A甲基化修饰响应植物生物胁迫和非生物胁迫的研究进展

doi:10.16420/j.issn.0513-353x.2022-0862

园艺学报 ›› 2023, Vol. 50 ›› Issue (9): 1841-1853.doi: 10.16420/j.issn.0513-353x.2022-0862

m⁶A甲基化修饰响应植物生物胁迫和非生物胁迫的研究进展

张盼¹, 余永旭², 曹领改¹, 朱广兵³, 吴伟³, 郭玉双¹, 尹国英¹, 贾蒙骜¹^,^*()

¹ 贵州省烟草科学研究院，烟草行业分子遗传重点实验室，贵阳 550081
² 贵州省烟草公司遵义市公司，贵州遵义 563000
³ 贵州省烟草公司六盘水市公司，贵州六盘水 553001

收稿日期:2022-11-17 修回日期:2023-05-17 出版日期:2023-09-25 发布日期:2023-09-26
通讯作者:
^*（E-mail：jiamengao@cau.edu.cn）
基金资助:
中国烟草总公司重点研发项目(110202102034); 中国烟草总公司贵州省公司科技项目(2020XM07); 中国烟草总公司贵州省公司科技项目(201903); 贵州省烟草科学研究院自立项目(GZYKY2021-05)

Research Progress of m⁶A Methylation Modification Response to Plant Biotic and Abiotic Stresses

ZHANG Pan¹, YU Yongxu², CAO Linggai¹, ZHU Guangbing³, WU Wei³, GUO Yushuang¹, YIN Guoying¹, JIA Meng’ao¹^,^*()

¹ Key Laboratory of Molecular Genetics，China National Tobacco Corporation，Guizhou Institute of Tobacco Science，Guiyang 550081，China
² Zunyi Branch of Guizhou Tobacco Companies，Zunyi，Guizhou 563000，China
³ Liupanshui Branch of Guizhou Tobacco Companies，Liupanshui，Guizhou 553001，China

Received:2022-11-17 Revised:2023-05-17 Published:2023-09-25 Online:2023-09-26
Contact: ^*（E-mail：jiamengao@cau.edu.cn）

摘要/Abstract

摘要：

N⁶-甲基腺苷（m⁶A）甲基化修饰是真核生物中一种重要的转录后表观修饰，可以丰富和调节真核生物的遗传信息。m⁶A甲基化修饰是由甲基转移酶（编码器，writer）、去甲基化酶（消码器，eraser）和m⁶A识别蛋白（读码器，reader）调节的动态可逆过程。综述了m⁶A甲基化修饰响应植物非生物胁迫和生物胁迫的研究进展，并展望了m⁶A甲基化修饰在植物应激反应研究中的热点，为更好地了解m⁶A甲基化修饰对植物响应外界不良环境的调控机制提供新的见解。

关键词: m⁶A, 甲基化, 生物胁迫, 非生物胁迫

Abstract:

N⁶-methyladenosine（m⁶A）RNA methylation is an important post-transcriptional epigenetic modification to enrich and regulate genetic information in eukaryotes. As a dynamic and reversible process，m⁶A modification is regulated by the methyltransferases（writers），demethylases（erasers），and recognition proteins（readers）. In this review，The research achievements of m⁶A methylation modification were summarized in plant biotic and abiotic stresses. The research hotspots of m⁶A methylation modification concerning the stress responses were also discussed. All of this could provide new insights about the underlying mechanisms of m⁶A modifications in regulating plant stress responses.

Key words: m⁶A, methylation, biotic stress, abiotic stress

张盼, 余永旭, 曹领改, 朱广兵, 吴伟, 郭玉双, 尹国英, 贾蒙骜. m⁶A甲基化修饰响应植物生物胁迫和非生物胁迫的研究进展[J]. 园艺学报, 2023, 50(9): 1841-1853.

ZHANG Pan, YU Yongxu, CAO Linggai, ZHU Guangbing, WU Wei, GUO Yushuang, YIN Guoying, JIA Meng’ao. Research Progress of m⁶A Methylation Modification Response to Plant Biotic and Abiotic Stresses[J]. Acta Horticulturae Sinica, 2023, 50(9): 1841-1853.

图/表 1

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非生物胁迫 Abiotic stress	功能 Function	基因 Gene	物种 Species	参考文献 Reference
干旱胁迫	编码器	OsMTA，OsMTB，OsVIR，	水稻Oryza sativa	Hu et al.,2019
Drought stress	Writer	PtrMTA	杨树Populus trichocarpa	Lu et al.,2020
	消码器	AtALKBH6	拟南芥Arabidopsis thaliana	Huong et al.,2020
	Eraser	OsALKBH1，OsALKBH6，OsALKBH8B， OsALKBH10A	水稻Oryza sativa	Hu et al.,2019
		ZmALKBH10A，ZmALKBH10B，	玉米Zea mays	Miao et al.,2020
		HrALKBH10B，HrALKBH10C，HrALKBH10D	沙棘Hippophae rhamnoides	Zhang et al.,2021a
	读码器	AtYTH06，AtYTH10	拟南芥Arabidopsis thaliana	Li et al.,2014a
	Reader	OsYTH11，OsYTH01，OsYTH02，OsYTH03，OsYTH05，OsYTH12，OsYTHDF2，OsYTHDF3，OsYTHDF10	水稻Oryza sativa	孙策,2018
		ZmECT2，ECT4	玉米Zea mays	Miao et al.,2020
		MhYTP1，MhYTP2	湖北海棠Malus hupehensis	Wang et al.,2017a,2017b
		CitYTH2，CitYTH3，CitYTH5，CitYTH7，CitYTH8，CitYTH9，CitYTH1，CitYTH4	甜橙Citrus sinensis	Ouyang et al.,2019
		CsYTH1	黄瓜Cucumis sativus	Zhou et al.,2018
		TaDF1a-4A，TaDF1a-4D，TaDF1a-4B，TaDF2a-5B，TaDF2a-5A，TaDF2a-5D，TaDF3a-5A，TaDF3a-5D，TaDF3a-5B，TaDF4a-5A，TaDF4a-5D，TaDF2b-1A，TaDF3c-4A，TaDF3c-4B，TaDF3c-4D	小麦Triticum aestivum	Sun et al.,2020
盐胁迫 Salt stress	编码器 Writer	OsMTA，OsMTB和OsVIR，OsMTA2，OsVIRILIZER，OsMTA4	水稻Oryza sativa	Hu et al.,2019；Wang et al.,2022
	消码器	AtMTA，AtMTB，AtVIR，AtHAKAI	拟南芥Arabidopsis thaliana	Hu et al.,2021
	Eraser	AtALKBH9A，AtALKBH6	拟南芥Arabidopsis thaliana	Merret et al.,2015； Huong et al.,2020
		OsALKBH9A，OsALKBH8，OsALKBH10A	水稻Oryza sativa	Wang et al.,2022
	读码器	AtYTHD10，	拟南芥Arabidopsis thaliana	Hu et al.,2019
	Reader	OsECT11，OsECT6，OsECT3，OsECT2，OsECT8，OsECT9，OsYTHDF3，OsYTHDF10，OsYTHDF4和OsYTHDF8	水稻Oryza sativa	孙策,2018；Wang et al.,2022
		MhYTP1，MhYTP2	湖北海棠Malus hupehensis	Wang et al.,2017a 2017b
		CitYTH4，CitYTH2，CitYTH6，CitYTH8，CitYTH5	甜橙Citrus sinensis	Ouyang et al.,2019
		CsYTH2，CsYTH5	黄瓜Cucumis sativus	Zhou et al.,2018
温度胁迫	编码器	AtMTA，AtFIP37	拟南芥Arabidopsis thaliana	Hu et al.,2019
Temperature	Writer	OsFIP，OsMTA，OsMTB，OsVIR	水稻Oryza sativa	Hu et al.,2019
stress		SlMTA，SlMTC，SlMTD，SlFIP37，SlHAKAI	番茄Solanum lycopersicum	Yang et al.,2021
	消码器 Eraser	AtALKBH10A，AtALKBH6	拟南芥Arabidopsis thaliana	Merret et al.,2015； Huong et al.,2020
		SlALKBH1B，SlALKBH2B，SlALKBH4B，SlALKBH6B	番茄Solanum lycopersicum	Yang et al.,2021
	读码器	SlECT1	番茄Solanum lycopersicum；	Yang et al.,2021
	Reader	AtYTHD05，AtYTHD06，AtYTHD07，AtYTHD10，AtYTHD08	拟南芥Arabidopsis thaliana	Huong et al.,2020
		OsYTHD05，OsYTHD06，OsYTHD07，OsYTHD09，OsYTHD03，OsYTHD08，OsYTHDF1	水稻Oryza sativa	Hu et al.,2019；孙策,2018
		MhYTP1，MhYTP2	湖北海棠Malus hupehensis	Wang et al.,2017a 2017b
		CitYTH2，CitYTH4，CitYTH5，CitYTH9，CitYTH3，CitYTH6，CitYTH7，CitYTH10，CitYTH8；	甜橙Citrus sinensis	Ouyang et al.,2019
		CsYTH1，CsYTH2，CsYTH4，	黄瓜Cucumis sativus	Zhou et al.,2018
		TaDF3a-5A，TaDF3a-5D，TaDF3a-5B，TaDF4a-5A，TaDF4a-5D，TaDF1b-7A，TaDF1b-7B，TaDF1b-7D，TaDF2b-1B，TaDF2b-1D，TaDF1c-2B，TaDF2c-3A，TaDF2c-3B，TaDF2c-3D，TaDF3c-4A，TaDF3c-4B，TaDF3c-4D	小麦Triticum aestivum	Sun et al.,2020
激素响应	消码器	AtALKBH6	拟南芥Arabidopsis thaliana	Huong et al.,2020
Response to hormone	Eraser	OsALKBH1，OsALKBH6，OsALKBH8B，OsALKBH10A	水稻Oryza sativa	Hu et al.,2019
	读码器 Reader	MhYTP1，MhYTP2	湖北海棠Malus hupehensis	Wang et al.,2017a 2017b
		CitYTH2，CitYTH10，CitYTH1，CitYTH4	甜橙Citrus sinensis	Ouyang et al.,2019
		CsYTH1，CsYTH3，CsYTH4，CsYT5	黄瓜Cucumis sativus	Zhou et al.,2018

非生物胁迫 Abiotic stress	功能 Function	基因 Gene	物种 Species	参考文献 Reference
干旱胁迫	编码器	OsMTA，OsMTB，OsVIR，	水稻Oryza sativa	Hu et al.,2019
Drought stress	Writer	PtrMTA	杨树Populus trichocarpa	Lu et al.,2020
	消码器	AtALKBH6	拟南芥Arabidopsis thaliana	Huong et al.,2020
	Eraser	OsALKBH1，OsALKBH6，OsALKBH8B， OsALKBH10A	水稻Oryza sativa	Hu et al.,2019
		ZmALKBH10A，ZmALKBH10B，	玉米Zea mays	Miao et al.,2020
		HrALKBH10B，HrALKBH10C，HrALKBH10D	沙棘Hippophae rhamnoides	Zhang et al.,2021a
	读码器	AtYTH06，AtYTH10	拟南芥Arabidopsis thaliana	Li et al.,2014a
	Reader	OsYTH11，OsYTH01，OsYTH02，OsYTH03，OsYTH05，OsYTH12，OsYTHDF2，OsYTHDF3，OsYTHDF10	水稻Oryza sativa	孙策,2018
		ZmECT2，ECT4	玉米Zea mays	Miao et al.,2020
		MhYTP1，MhYTP2	湖北海棠Malus hupehensis	Wang et al.,2017a,2017b
		CitYTH2，CitYTH3，CitYTH5，CitYTH7，CitYTH8，CitYTH9，CitYTH1，CitYTH4	甜橙Citrus sinensis	Ouyang et al.,2019
		CsYTH1	黄瓜Cucumis sativus	Zhou et al.,2018
		TaDF1a-4A，TaDF1a-4D，TaDF1a-4B，TaDF2a-5B，TaDF2a-5A，TaDF2a-5D，TaDF3a-5A，TaDF3a-5D，TaDF3a-5B，TaDF4a-5A，TaDF4a-5D，TaDF2b-1A，TaDF3c-4A，TaDF3c-4B，TaDF3c-4D	小麦Triticum aestivum	Sun et al.,2020
盐胁迫 Salt stress	编码器 Writer	OsMTA，OsMTB和OsVIR，OsMTA2，OsVIRILIZER，OsMTA4	水稻Oryza sativa	Hu et al.,2019；Wang et al.,2022
	消码器	AtMTA，AtMTB，AtVIR，AtHAKAI	拟南芥Arabidopsis thaliana	Hu et al.,2021
	Eraser	AtALKBH9A，AtALKBH6	拟南芥Arabidopsis thaliana	Merret et al.,2015； Huong et al.,2020
		OsALKBH9A，OsALKBH8，OsALKBH10A	水稻Oryza sativa	Wang et al.,2022
	读码器	AtYTHD10，	拟南芥Arabidopsis thaliana	Hu et al.,2019
	Reader	OsECT11，OsECT6，OsECT3，OsECT2，OsECT8，OsECT9，OsYTHDF3，OsYTHDF10，OsYTHDF4和OsYTHDF8	水稻Oryza sativa	孙策,2018；Wang et al.,2022
		MhYTP1，MhYTP2	湖北海棠Malus hupehensis	Wang et al.,2017a 2017b
		CitYTH4，CitYTH2，CitYTH6，CitYTH8，CitYTH5	甜橙Citrus sinensis	Ouyang et al.,2019
		CsYTH2，CsYTH5	黄瓜Cucumis sativus	Zhou et al.,2018
温度胁迫	编码器	AtMTA，AtFIP37	拟南芥Arabidopsis thaliana	Hu et al.,2019
Temperature	Writer	OsFIP，OsMTA，OsMTB，OsVIR	水稻Oryza sativa	Hu et al.,2019
stress		SlMTA，SlMTC，SlMTD，SlFIP37，SlHAKAI	番茄Solanum lycopersicum	Yang et al.,2021
	消码器 Eraser	AtALKBH10A，AtALKBH6	拟南芥Arabidopsis thaliana	Merret et al.,2015； Huong et al.,2020
		SlALKBH1B，SlALKBH2B，SlALKBH4B，SlALKBH6B	番茄Solanum lycopersicum	Yang et al.,2021
	读码器	SlECT1	番茄Solanum lycopersicum；	Yang et al.,2021
	Reader	AtYTHD05，AtYTHD06，AtYTHD07，AtYTHD10，AtYTHD08	拟南芥Arabidopsis thaliana	Huong et al.,2020
		OsYTHD05，OsYTHD06，OsYTHD07，OsYTHD09，OsYTHD03，OsYTHD08，OsYTHDF1	水稻Oryza sativa	Hu et al.,2019；孙策,2018
		MhYTP1，MhYTP2	湖北海棠Malus hupehensis	Wang et al.,2017a 2017b
		CitYTH2，CitYTH4，CitYTH5，CitYTH9，CitYTH3，CitYTH6，CitYTH7，CitYTH10，CitYTH8；	甜橙Citrus sinensis	Ouyang et al.,2019
		CsYTH1，CsYTH2，CsYTH4，	黄瓜Cucumis sativus	Zhou et al.,2018
		TaDF3a-5A，TaDF3a-5D，TaDF3a-5B，TaDF4a-5A，TaDF4a-5D，TaDF1b-7A，TaDF1b-7B，TaDF1b-7D，TaDF2b-1B，TaDF2b-1D，TaDF1c-2B，TaDF2c-3A，TaDF2c-3B，TaDF2c-3D，TaDF3c-4A，TaDF3c-4B，TaDF3c-4D	小麦Triticum aestivum	Sun et al.,2020
激素响应	消码器	AtALKBH6	拟南芥Arabidopsis thaliana	Huong et al.,2020
Response to hormone	Eraser	OsALKBH1，OsALKBH6，OsALKBH8B，OsALKBH10A	水稻Oryza sativa	Hu et al.,2019
	读码器 Reader	MhYTP1，MhYTP2	湖北海棠Malus hupehensis	Wang et al.,2017a 2017b
		CitYTH2，CitYTH10，CitYTH1，CitYTH4	甜橙Citrus sinensis	Ouyang et al.,2019
		CsYTH1，CsYTH3，CsYTH4，CsYT5	黄瓜Cucumis sativus	Zhou et al.,2018

m⁶A甲基化修饰响应植物生物胁迫和非生物胁迫的研究进展

Research Progress of m⁶A Methylation Modification Response to Plant Biotic and Abiotic Stresses

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