OT百合杂交育种历程中的花色演变分析

doi:10.16420/j.issn.0513-353x.2021-0578

摘要/Abstract

摘要：

OT百合杂种系（Oriental × Trumpet）花色种类丰富,花部呈色模式新颖,是目前国际上百合育种的主要方向。对世界范围内培育和登录的OT百合品种（1958—2018年）的花色和育种信息进行整理和分类,分析了OT百合杂交育种的历史,计算参与OT百合起源的重要亲本的核遗传贡献值;系统研究了OT百合色系和类型。OT百合品种共有8个色系和5种着色模式;不同色系主要起源于湖北百合（Lilium henryi）、鹿子百合（L. speciosum）、乙女百合（L. rubellum）和天香百合（L. auratum）等,这4个亲本的总核遗传贡献值分别为10.3035、6.7955、4.1250和2.5255;在杂交育种中出现花朵色块嵌合现象,色素合成途径的互作和染色体变异的演变模式是形成OT百合丰富花色的原因。

关键词: 百合, OT杂种系, 花色, 遗传, 育种历程

Abstract:

Among the lily hybrids,the Lilium（Oriental × Trumpet）OT hybrids has the abundant variations in flower color and pigmentation patterning,which has become the main direction of lily breeding in the world. The flower color and breeding information of OT varieties cultivated and registered in the world（1958—2018）were sorted out and classified,the history of hybrid breeding of OT hybrids was analyzed,and the contribution value of important parents involved in the origin of OT hybrids was calculated. The breeding process of OT hybrids with various color systems and flower color types was systematically researched. The results showed that there were eight color systems and five kinds of pigmentation patterning in OT hybrids. The different color systems mainly originated from L. henryi,L. speciosum,L. rubellum and L. auratum with the total contribution value of 10.3035,6.7955,4.1250 and 2.5255. The mosaic phenomenon of flower color patterning,the interaction of pigment synthesis pathway and the evolution pattern of chromosome variation in the process of hybridization breeding are the reasons for the formation of abundant flower color.

Key words: Lilium, OT hybrids, flower coloration, inheritance, breeding process

中图分类号:

S682.2

余鹏程, 谭平宇, 高丽, 贾桂霞. OT百合杂交育种历程中的花色演变分析[J]. 园艺学报, 2021, 48(10): 1885-1894.

YU Pengcheng, TAN Pingyu, GAO Li, JIA Guixia. Analysis of the Development of Flower Color Based on Hybridization Breeding Process in Lilium OT Hybrids[J]. Acta Horticulturae Sinica, 2021, 48(10): 1885-1894.

图/表 6

参考文献 23

[1]	Anonymouos. 1964. A proposed horticultural classification of lilies for registration,show and catalogue purposes. The Lily Year Book,Royal Horticultural Society, 27:162-164.
[2]	Baker J G. 1888. Lilium(Archelirion)henryi. The Gardeners’ Chronicle 4,series 3:660.
[3]	Baker J G. 1894. Lilium brownii var. leucanthum,Baker. The Gardeners’ Chronicle 16,series 3:180.
[4]	Cao Q Z, Lian Y Q, Wang L J, Zhang Q, Zhao Y Q, Jia G X, He H B. 2019. Physical mapping of 45S rDNA loci in Lilium OT hybrids and interspecific hybrids with Lilium regale. Scientia Horticulturae, 252:48-54. doi: 10.1016/j.scienta.2019.03.035 URL
[5]	Darby G W. 1965. Lilium hybrid‘Black Beauty’. The Lily Year Book,Royal Horticultural Society, 28:118.
[6]	Du Ling-juan, Chen Kai-li, Liu Ya-li. 2017. Cloning of flavonol synthase gene(FLS1) and relativity analysis of its expression with the flower color in grape hyacinth. Journal of Northwest Forestry University, 32 (1):106-113.(in Chinese)
	杜灵娟, 陈凯利, 刘雅莉. 2017. 葡萄风信子FLS1基因克隆及其表达与花色性状之间的关联性分析. 西北林学院学报, 32 (1):106-113.
[7]	Emsweller S L, Uhring J. 1966. Lilium ×‘Black Beauty’-diploid and amphidiploid. The Lily Year Book,Royal Horticultural Society, 29:45-47.
[8]	Gai Jun-yi, Zhao Tuan-jie, Cui Zhang-lin, Qiu Jia-xun. 1998. Genetic contribution of Germplasm from different geographical sources in Chinese soybean cultivars. Scientia Agriculture Sinica, 31 (5):35-43.(in Chinese)
	盖钧镒, 赵团结, 崔章林, 邱家驯. 1998. 中国大豆育成品种中不同地理来源种质的遗传贡献. 中国农业科学, 31 (5):35-43.
[9]	Li C, Bai Y, Li S, Chen H, Han X, Zhao H, Shao J, Park S, Wu Q. 2012. Cloning,Characterization,and activity analysis of a flavonol synthase gene FtFLS1 and its association with flavonoid content in Tartary Buckwheat. J Agric Food Chem, 60 (20):5161-5168. doi: 10.1021/jf205192q URL
[10]	Long Ya-yi, Zhang Jin-zheng, Zhang Lan-nian. 1999. Lilium-the king of bulbous flowers. Beijing: Golden Shield Press.(in Chinese)
	龙雅宜, 张金政, 张兰年. 1999. 百合--球根花卉之王. 北京: 金盾出版社.
[11]	Marasek-Ciolakowska A, Nishikawa T, Shea D J, Okazaki K. 2018. Breeding of lilies and tulips-Interspecific hybridization and genetic background. Breeding Science, 68 (1):35-52. doi: 10.1270/jsbbs.17097 pmid: 29681746
[12]	Matthews V. 2007. The international lily register and checklist. Royal Horticultural Society.
[13]	Okubo H. 2014. History of Lilium species in Asia. Acta Horticulturae, 1027:11-26.
[14]	Ramanna M S, Marasek-Ciolakowska A, Xie S, Khan N, Arens P, van Tuyl J M. 2012. The significance of polyploidy for bulbous ornamentals:a molecular cytogenetic assessment. Floric Ornam Biotech, 6:116-121.
[15]	Ronald W G, Ascher P D. 1976. Lilium ×‘Black Beauty’-a potential bridging hybrid in Lilium. Euphytica, 25 (1):285-291. doi: 10.1007/BF00041558 URL
[16]	Shimizu M. 1971. Lilies of Japan. Tokyo:Seibundo Shinko-sha.(in Japanese)
[17]	Toledo L A, Bennett M D, Stern H. 1979. Cytological investigations of the effect of colchicine on meiosis in Lilium hybrid cv.‘Black Beauty’microsporocytes. Chromosoma, 72 (2):157-173. doi: 10.1007/BF00293232 URL
[18]	Wang Hao-bo, Wang Ming, Gao Xiu-wu, Yu Zeng-liang. 2003. Breeding history and germplasm basis of watermelon cultivars in America. Acta Horticulturae Sinica, 30 (3):366-366.(in Chinese)
	王浩波, 王鸣, 高秀武, 余增亮. 2003. 美国西瓜育种历程及育成品种的种质基础. 园艺学报, 30 (3):366-366.
[19]	Wilson E H. 1925. The lilies of eastern Asia. Dalau & Co.,London:28-29.
[20]	Woodriff L. 1958. Lily breeding notes. Lily Yearb North Am Lily Soc, 11:33-35.
[21]	Yamagishi. 2013. How genes paint lily flowers:regulation of colouration and pigmentation patterning. Scientia Horticulturae, 163 (Complete):27-36. doi: 10.1016/j.scienta.2013.07.024 URL
[22]	Zheng Si-xiang, Mao Wei-wei, Wang Li-long, Zhang Lin, Liao Xiao-shan, Song Zhi-wei, Lin Qing-dan, Long Bing, Hu Song-ping, Wu Jia-kai. 2017. Cytological observation on distant hybrid progenies of OT lily and Oriental lily. Journal of Jiangxi Agricultural University, 39 (6):1082-1088.(in Chinese)
	郑思乡, 毛伟伟, 王利龙, 张琳, 廖晓珊, 宋志伟, 林庆丹, 龙彬, 胡颂平, 吴嘉垲. 2017. OT型百合与东方百合远缘杂交后代的细胞学观察. 江西农业大学学报, 39 (6):1082-1088.
[23]	Zhou Yan-ping. 2020. Study on genetic diversity and genetic relationship of Lilium[Ph. D. Dissertation]. Beijing:Beijing Forestry University.(in Chinese)
	周艳萍. 2020. 百合遗传多样性和亲缘关系研究[博士论文]. 北京: 北京林业大学.

花朵主要颜色 Color of the floral center	品种数 Number of varieties	品种数占比/% Percentage of varieties	花朵主要颜色 Color of the floral center	品种数 Number of varieties	品种数占比/% Percentage of varieties
白色White	38	7.8	橙红色Orange red	7	1.4
黄色Yellow	108	22.1	红色Red	79	16.2
黄绿色Greenish yellow	54	11.0	粉色Pink	66	13.5
橙色Orange	22	4.5	紫红色Purplish red	99	20.2

花朵主要颜色 Color of the floral center	品种数 Number of varieties	品种数占比/% Percentage of varieties	花朵主要颜色 Color of the floral center	品种数 Number of varieties	品种数占比/% Percentage of varieties
白色White	38	7.8	橙红色Orange red	7	1.4
黄色Yellow	108	22.1	红色Red	79	16.2
黄绿色Greenish yellow	54	11.0	粉色Pink	66	13.5
橙色Orange	22	4.5	紫红色Purplish red	99	20.2

野生种 Wild speices	贡献值 Value	贡献率/% Percent	品种数 Number of cultivars
湖北百合 Lilium henryi	10.3035	36.7	40
鹿子百合 L. speciosum	6.7955	24.2	27
乙女百合 L. rubellum	4.1250	14.7	12
天香百合 L. auratum	2.5255	9.0	12
紫脊百合 L. leucanthum var. centifolium	1.7625	6.3	13
宜昌百合 L. leucanthum	0.5750	2.0	5
通江百合 L. sargentiae	0.5000	1.8	1
岷江百合 L. ragele	0.5000	1.8	1
淡黄花百合 L. sulphureum	0.5000	1.8	1
白色鹿子百合 L. speciosum var. album	0.5000	1.8	1

野生种 Wild speices	贡献值 Value	贡献率/% Percent	品种数 Number of cultivars
湖北百合 Lilium henryi	10.3035	36.7	40
鹿子百合 L. speciosum	6.7955	24.2	27
乙女百合 L. rubellum	4.1250	14.7	12
天香百合 L. auratum	2.5255	9.0	12
紫脊百合 L. leucanthum var. centifolium	1.7625	6.3	13
宜昌百合 L. leucanthum	0.5750	2.0	5
通江百合 L. sargentiae	0.5000	1.8	1
岷江百合 L. ragele	0.5000	1.8	1
淡黄花百合 L. sulphureum	0.5000	1.8	1
白色鹿子百合 L. speciosum var. album	0.5000	1.8	1

品种/系 Cultivar/group	贡献值 Value	贡献率/% Percent	参与的品种数 Number of cultivars	品种/系 Cultivar/group	贡献值 Value	贡献率/% Percent	参与的品种数 Number of cultivars
‘Black Beauty’（OT）	5.00	16.0	12	‘Flashpoint’（OT）	0.50	1.6	1
‘Canterbury Belle’（OT）	3.75	12.0	8	‘Val di Sole’（O）	0.50	1.6	1
‘Starburst Sensation’（OT）	3.50	11.2	10	‘Siberia’（O）	0.50	1.6	2
‘White Henry’（T）	2.75	8.8	11	‘Casa Blanca’（O）	0.50	1.6	1
‘Aubade’（O）	2.50	8.0	5	‘Conca d’Or’（OT）	0.50	1.6	1
L. parkmanii group（O）	1.50	4.8	3	‘Rachel Pappo’（OT）	0.50	1.6	1
‘Leslie Woodriff’（OT）	1.00	3.2	2	‘Star Gazer’（O）	0.50	1.6	1
‘Moonlight’group（T）	1.00	3.2	2	‘Thunderbolt’（T）	0.50	1.6	1
‘Yelloween’（OT）	1.00	3.2	2	‘Pink Jewel’（O）	0.50	1.6	1
‘Northern Carillon’ （OT）	1.00	3.2	2	‘Tetra Journey’（O）	0.50	1.6	1
‘Dominique’（O）	0.75	2.4	6	‘Damson’（T）	0.50	1.6	1
‘Heads Up’（T）	0.50	1.6	1	‘Bel Air’（O）	0.25	0.8	1
‘Mero Star’（O）	0.50	1.6	1	‘ML’s Indian Summer’	0.25	0.8	1
‘Thomas’s Giant’(T)	0.50	1.6	1